Stem cell passage affects directional migration of stem cells in electrotaxis

Hong, Seok Jin; Lee, Mi Hee; Koo, Min Ah; Seon, Gyeung Mi; Park, Ye Jin; Kim, Dohyun; Park, Jong Chul

doi:10.1016/j.scr.2019.101475

Cited by 24 publications

(23 citation statements)

References 33 publications

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“…The impact of long-term cultivation on cellular fitness is well documented in MSC: at a pre-senescent state, self-renewal and migratory properties drastically decline 18 , 36 , 37 . The migratory potential can be enhanced in old MSC by specific cytokines 38 , only one being interleukin-6 (IL6).…”

Section: Discussionmentioning

confidence: 99%

Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

Marozin

Simon-Nobbe

Irausek

et al. 2021

Sci Rep

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The human fetal osteoblast cell line (hFOB 1.19) has been proposed as an accessible experimental model for study of osteoblast biology relating to drug development and biomaterial engineering. For their multilineage differentiation potential, hFOB has been compared to human mesenchymal progenitor cells and used to investigate bone-metabolism in vitro. Hereby, we studied whether and to what extent the conditionally immortalized cell line hFOB 1.19 can serve as a surrogate model for bone-marrow derived mesenchymal stromal cells (bmMSC). hFOB indeed exhibit specific characteristics reminiscent of bmMSC, as colony formation, migration capacity and the propensity to grow as multicellular aggregates. After prolonged culture, in contrast to the expected effect of immortalization, hFOB acquired a delayed growth rate. In close resemblance to bmMSC at increasing passages, also hFOB showed morphological abnormalities, enlargement and finally reduced proliferation rates together with enhanced expression of the cell cycle inhibitors p21 and p16. hFOB not only have the ability to undergo multilineage differentiation but portray several important aspects of human bone marrow mesenchymal stromal cells. Superior to primary MSC and osteoblasts, hFOB enabled the generation of continuous cell lines. These provide an advanced basis for investigating age-related dysfunctions of MSCs in an in vitro 3D-stem cell microenvironment.

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Section: Discussionmentioning

confidence: 99%

Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

Marozin

Simon-Nobbe

Irausek

et al. 2021

Sci Rep

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“…In other words, although cell groups were exposed to ES for an equivalent time period (continuous ES for 6 h as opposed to two repetitions of 3 h ES each), the repetition of shorter bursts of ES was more effective in maintaining the cell migration direction without causing cell damage. We had previously shown that human tonsil-, adipose tissue-, and bone marrowderived mesenchymal stem cells (MSC) migrate directly toward the anode when exposed to an electric current [24,25]. Other published studies have also indicated that either direct or alternating current stimulate the directional migration of cells toward a specific cathodal or anodal orientation [23,[36][37][38][39][40].…”

Section: Resultsmentioning

confidence: 99%

“…During electrotaxis, cells subjected to direct electric current will move preferentially toward either the anode or cathode, indicating that the effects of electric signals on cells are specific to different cell types and species [ 21 , 22 , 23 ]. Researchers have previously demonstrated that exogenous direct ES can effectively guide directional migration toward the anode of ADSCs, human mesenchymal stem cells (hMSCs), and tonsil derived MSCs [ 24 , 25 , 26 ]. In our previous studies, we showed that cells migrate toward the cathode or anode following Golgi apparatus (GA) polarization, in the same direction as the ES.…”

Section: Introductionmentioning

confidence: 99%

Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

Lee

Park

Hong

et al. 2021

Cells

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Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.

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“…La utilización de las CM en terapia regenerativa ha aumentado en laúltima década gracias a su capacidad de diferenciarse hacia varios tejidos (Cui et al 2017); lo anterior se evidencia en una búsqueda en el banco de datos del Instituto Nacional de Salud de Estados Unidos (https://www.clinicaltrials.gov); utilizando el término Mesenchymal stem cell, el 10-06-2019 se encontraron 757 estudios clínicos al respecto, variando entre trasplante para enfermedades pulmonares y diabetes (Can et al 2017), reconstrucción del epitelio corneal (Ziaei et al 2017), tratamientos en Alzheimer (Wang et al 2018), etc. No obstante, a nivel in vitro, las CM necesitan ser estimuladas para prevenir el envejecimiento prematuro, también conocido como senescencia replicativa (de Magalhães y Passos 2018, Hong et al 2019). Debido a que con el aumento del número de pasajes las características proliferativas y el potencial de diferenciación de las CM se van perdiendo, su uso en terapia celular se ve afectado (Guo et al 2010, Hong et al 2019, Zhai et al 2019. En este proceso, el FGF-2 juega un papel estratégico al desencadenar la activación de cascadas intracelulares, responsables de la proliferación y diferenciación que potencian la vida del cultivo (Lai et al 2011).…”

Section: Fgf-2 Y Su Implicación En La Superviven-cia De Células Madreunclassified

Influencia del factor de crecimiento fibroblástico 2 en células madre in vitro

Vargas¹,

Arzuza²,

Turner³

2020

Actual. Biol.

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El interés y la necesidad de estudiar las células madre en terapias regenerativas han aumentado en los últimos años debido a su capacidad de proliferación y diferenciación hacia múltiples linajes. Desafortunadamente, el número de células demandado para un trasplante satisfactorio es mayor al que se logra extraer directamente del paciente, por lo que se deben realizar cultivos in vitro de células madre. Sin embargo, con el tiempo las células de estos cultivos se vuelven senescentes, disminuyendo así su número de divisiones celulares y su capacidad proliferativa. Para solucionar esta problemática se han propuesto factores de crecimiento como agentes potenciadores de la proliferación celular. Entre estos se encuentra el factor de crecimiento fibroblástico 2, que al agregarse al medio podría promover la proliferación y aumentar el tiempo de vida de las células en cultivo. En la siguiente revisión se recopila información sobre la biología de este factor de crecimiento y el tipo de señalización que utiliza, al igual que sus aplicaciones en terapia regenerativa y su efecto en la proliferación celular y senescencia.

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Stem cell passage affects directional migration of stem cells in electrotaxis

Cited by 24 publications

References 33 publications

Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

Influencia del factor de crecimiento fibroblástico 2 en células madre in vitro

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