Stem cell responses to nanotopography

Park, Siyeon; Im, Gun‐Il

doi:10.1002/jbm.a.35236

Cited by 43 publications

(20 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have previously demonstrated that BMPC growth was equivalent on tissue culture surfaces or PFVH‐CH‐B scaffold, suggesting that in the present study the differences in adhesion and proliferation may be influenced by surface characteristics rather than by the chemical nature of the scaffold. In agreement with these results, we and other groups have demonstrated that the topography of the substratum may influence cell attachment, proliferation and commitment . Moreover, surface characteristic influences also cell spreading and cytoskeleton rearrangement which in turn could influence the differentiation potential of BMPC …”

Section: Resultssupporting

confidence: 89%

Nanostructured fumarate copolymer‐chitosan crosslinked scaffold: Anin vitroosteochondrogenesis regeneration study

Lastra

Molinuevo

Blaszczyk-Lezak

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

In the tissue engineering field, the design of the scaffold inspired on the natural occurring tissue is of vital importance. Ideally, the scaffold surface must promote cell growth and differentiation, while promote angiogenesis in the in vivo implant of the scaffold. On the other hand, the material selection must be biocompatible and the degradation times should meet tissue reparation times. In the present work, we developed a nanofibrous scaffold based on chitosan crosslinked with diisopropylfumarate-vinyl acetate copolymer using anodized aluminum oxide (AAO) templates. We have previously demonstrated its biocompatibility properties with low cytotoxicity and proper degradation times. Now, we extended our studies to demonstrate that it can be successfully nanostructured using the AAO templates methodology, obtaining a nanorod-like scaffold with a diameter comparable to those of collagen fibers of the bone matrix (170 and 300 nm). The nanorods obtained presented a very homogeneous pattern in diameter and length, and supports cell attachment and growth. We also found that both osteoblastic and chondroblastic matrix production were promoted on bone marrow progenitor cells and primary condrocytes growing on the scaffolds, respectively. In addition, the nanostructured scaffold presented no cytotoxicity as it was evaluated using a model of macrophages on culture. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 570-579, 2018.

show abstract

Section: Resultssupporting

confidence: 89%

Nanostructured fumarate copolymer‐chitosan crosslinked scaffold: Anin vitroosteochondrogenesis regeneration study

Lastra

Molinuevo

Blaszczyk-Lezak

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…The major effects of topography on cells are cell adhesion, proliferation, morphological changes, migration, and organization, which ultimately helps determine cell fate [65]. The ranges of topographical cues investigated span from the micro to the nano scale.…”

Section: Biophysical Regulation Of Cell Reprogrammingmentioning

confidence: 99%

A biomaterial approach to cell reprogramming and differentiation

Long

Kim

et al. 2017

J. Mater. Chem. B

View full text Add to dashboard Cite

Cell reprogramming of somatic cells into pluripotent states and subsequent differentiation into certain phenotypes has helped progress regenerative medicine research and other medical applications. Recent research has used viral vectors to induce this reprogramming; however, limitations include low efficiency and safety concerns. In this review, we discuss how biomaterial methods offer potential avenues for either increasing viability and downstream applicability of viral methods, or providing a safer alternative. The use of non-viral delivery systems, such as electroporation, micro/nanoparticles, nucleic acids and the modulation of culture substrate topography and stiffness have generated valuable insights regarding cell reprogramming.

show abstract

“…Some reports describe the relationship between the orientation of cell pseudopodia and nanostructure . However, there is still not a uniform conclusion to expound the mechanism of how the nano‐topographic structures have an effect on cells …”

Section: Resultsmentioning

confidence: 99%

Nanostructured Multilayer Films Assembled from Poly(dopamine)‐Coated Carbon Nanotubes for Controlling Cell Behavior

Ren

Zhang

et al. 2017

ChemNanoMat

View full text Add to dashboard Cite

Nano‐topographic surfaces have been used as an effective tool to control cell behavior such as adhesion and proliferation. In this study, multilayer films with nano‐topographic features were fabricated by alternatively assembling poly(l‐lysine) (PLL) and poly(dopamine)‐coated carbon nanotubes (CNTs@PDA) layers. The growth of PLL/CNTs@PDA film presented a perfect linear relationship with the number of bilayers. A nanostructured morphology with interpenetrating CNT networks was observed by scanning electron microscopy (SEM). Adhesion and proliferation of endothelial cells (ECs) and smooth muscle cells (SMCs) on the PLL/CNTs@PDA multilayer films have been evaluated. The films support initial adhesion of both ECs and SMCs. Interestingly, the PLL/CNTs@PDA multilayer films were found to promote proliferation of SMCs and inhibited proliferation of ECs. Further, pheochromocytoma (PC12) cells were employed to evaluate the influence of PLL/CNTs@PDA multilayer films on the outgrowth of synapses. We found that the nanostructured surface significantly promoted the synapses of PC12 cell growth and formation. Our findings suggest that cytophilic surfaces with the nanostructured morphology have diverse effects on different cells, which sheds light on new design of biomaterial surfaces in cell‐based applications.

show abstract

Stem cell responses to nanotopography

Cited by 43 publications

References 62 publications

Nanostructured fumarate copolymer‐chitosan crosslinked scaffold: Anin vitroosteochondrogenesis regeneration study

Nanostructured fumarate copolymer‐chitosan crosslinked scaffold: Anin vitroosteochondrogenesis regeneration study

A biomaterial approach to cell reprogramming and differentiation

Nanostructured Multilayer Films Assembled from Poly(dopamine)‐Coated Carbon Nanotubes for Controlling Cell Behavior

Contact Info

Product

Resources

About