Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria

Köhl, J.; Postma, J.; Nicot, Philippe C.; Ruocco, Michelina; Blum, Bernard

doi:10.1016/j.biocontrol.2010.12.004

Cited by 201 publications

(137 citation statements)

References 42 publications

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“…As the production of these secondary metabolites is known, results depend largely on the nutritional and specific environmental conditions (Köhl et al, 2011), results that have been demonstrated under the in vitro conditions of the present study, showing differences in antagonism depend on the culture medium used.…”

Section: Discussionsupporting

confidence: 57%

Vanilla Rhizobacteria as Antagonists against Fusarium oxysporum f. sp. vanillae

Adame-García

Luna-Rodríguez

Iglesias-Andreu

2015

IJAB

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Pathogens such as Fusarium oxysporum strongly affect the health of various agricultural crops like vanilla. However, despite significant economic losses caused by this pathogen there is no efficient method for its control. Therefore, we propose using rhizobacteria obtained from vanilla roots against F. oxysporum f. sp. vanillae. The results showed that there was no positive correlation between the antagonism expressed under in vitro conditions and those expressed under greenhouse conditions. The 16S rDNA gene analysis indicated that the bacterial genera tested corresponded to Sphingobacterium, Staphylococcus, Serratia, Psychrobacter, Pseudomonas and Stenotrophomonas. The in vitro antifungal activity was evaluated using three culture media (Potato Dextrose Agar, Nutrient Agar and Czapek) using the empty box technique (antagonism). Isolates of Staphylococcus xylosus BAC-JAG15, Serratia sp. BAC-JAG4 and Stenotrophomonas sp. BAC-JAG1 showed 90% in vitro antagonism against F. oxysporum in the three media tested. In the greenhouse evaluation, plants treated with these isolates initially showed symptoms of chlorosis without developing characteristic symptoms of disease produced by F. oxysporum f. sp. vanillae. This demonstrates protection provided by rhizobacteria against infection from F. oxysporum f. sp. vanillae in vanilla plants.

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Section: Discussionsupporting

confidence: 57%

Vanilla Rhizobacteria as Antagonists against Fusarium oxysporum f. sp. vanillae

Adame-García

Luna-Rodríguez

Iglesias-Andreu

2015

IJAB

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“…These strains were further selected for in vitro antagonistic activity against the pathogen (Díaz et al 2013) and competition for nutrients (Blumenstein et al 2015), but field trials with elm trees with four of these strains showed no repeatable control of DED (Martín et al 2015a). Selecting an effective, environmentally safe and commercial valuable biocontrol agent requires many crucial choices (Köhl et al 2011). Costs of production and application of Dutch Trig Ò are relatively high and the shelf-life is limited.…”

Section: Biological Control Of Ded With Dutch Trigmentioning

confidence: 99%

Twenty-four years of Dutch Trig® application to control Dutch elm disease

Postma

Geijn

2016

BioControl

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Since 1992 elm trees have been treated with a biological control product Dutch Trig Ò to protect them from infection by Ophiostoma novo-ulmi causing Dutch elm disease (DED). The active ingredient of the biocontrol product consists of the fungus Verticillium albo-atrum strain WCS850. A conidiospore suspension of this fungus is injected into the vascular system of elm trees at a height of 1.3 m. This biocontrol product prevents healthy elm trees from fungal infection transmitted by elm bark beetles. Dutch Trig Ò , however, does not protect already infected trees or trees connected with diseased trees via root grafts. Since 2010, only 0.1 % of the injected elms became infected with DED through beetle transmission and an additional 0.4 % of the treated elms were infected through root contact in the Netherlands. Regression analysis considering all injected elm trees in the Netherlands since 1992 indicated that DED infection through beetle transmission had significantly decreased during the 24 years application of Dutch Trig Ò . In 2015, approximately 28,300 trees in five countries (Netherlands, USA, Germany, Canada and Sweden) were treated with Dutch TrigÒ to protect valuable susceptible elm trees, mainly in urban environments.

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“…In this paper we will not describe the process of collection, evaluation, development of mass production and registration of biological control agents in detail. Information concerning these factors for invertebrate biological control agents can be found in Cock et al (2010) and for microbial biological control agents in Köhl et al (2011), Ravensberg (2011) and Parnell et al (2016). When searching for natural enemies, it is not unusual to find dozens or more species attacking a certain pest, but criteria such as population growth rate, host range, and adaptation to crop and climate can often be used to quickly eliminate clearly inefficient species.…”

Section: Introductionmentioning

confidence: 99%

Biological control using invertebrates and microorganisms: plenty of new opportunities

et al. 2017

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In augmentative biological control (ABC), invertebrate and microbial organisms are seasonally released in large numbers to reduce pests. Today it is applied on more than 30 million ha worldwide. Europe is the largest commercial market for invertebrate biological control agents, while North America has the largest sales of microbials. A strong growth in use of ABC, particularly of microbial agents, is taking place in Latin America, followed by Asia. The current popularity of ABC is due to (1) its inherent positive characteristics (healthier for farm workers and persons living in farming communities, no harvesting interval or waiting period after release of agents, sustainable as there is no development of resistance against arthropod natural enemies, no phytotoxic damage to plants, better yields and a healthier product, reduced pesticide residues [well below the legal Maximum Residue Levels (MRLs)], (2) professionalism of the biological control industry (inexpensive large scale mass production, proper quality control, efficient packaging, distribution and release methods, and availability of many ([440 species) control agents for numerous pests), (3) a number of recent successes showing how biological control can save agricultural production when pesticides fail or are not available, (4) several Handling Editor: Russell Messing.Electronic supplementary material The online version of this article

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Stepwise screening of microorganisms for commercial use in biological control of plant-pathogenic fungi and bacteria

Cited by 201 publications

References 42 publications

Vanilla Rhizobacteria as Antagonists against Fusarium oxysporum f. sp. vanillae

Vanilla Rhizobacteria as Antagonists against Fusarium oxysporum f. sp. vanillae

Twenty-four years of Dutch Trig® application to control Dutch elm disease

Biological control using invertebrates and microorganisms: plenty of new opportunities

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