Steroid Inhibition of Leukocyte Defense System in Mycoplasmal Uveitis

Pavan-Langston, Deborah; Geary, Patricia A.

doi:10.1001/archopht.1971.01000010577017

Cited by 3 publications

(2 citation statements)

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“…At present, virtually nothing is known about the immunological mechanisms by which the eye resists infection, although recently work has been done on viral keratitis and auto-immune induced uveitis [9], Previous murine experimental studies by Gerke and Magliocco [4] and others [7,10] indicated that infected mice recovered from eye in fections after 2-3 weeks duration. However, this kind of experimental model is not characteristic of the severe eye response seen in man.…”

Section: Discussionmentioning

confidence: 99%

Experimental Eye Infections Caused by Pseudomonas aeruginosa

1976

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Cells of Pseudomonas aeruginosa were titered (1.0× 10⁴–1.0× 10⁸ cells) to determine the minimum number required to initiate corneal infection in Swiss-Webster and BALB/c mice. No infection was produced in either strain of mouse by direct application of the organisms to unwounded corneas. However, wounding the cornea in either strain followed by application of the bacteria (1 × 10⁷–1 × 10⁸ cells) produced corneal infection within 18–24 h. In Swiss-Webster mice, corneal morphology appeared normal within 4–6 weeks. However, in BALB/c mice, changes occurred by 12–15 days post-infection resulting in micro-ophthalmia. The results suggest development of an experimental model to comparatively study the natural immune mechanism of the eye to bacterial challenge.

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Section: Discussionmentioning

confidence: 99%

Experimental Eye Infections Caused by Pseudomonas aeruginosa

1976

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“…This finding concurs with the previous report that demonstrates the inhibitory effect of GC on lymphocytes as the treatment of mycoplasma uveitis using Mycoplasma pulmonis inoculation in the anterior chamber. 34 In our previous report, 35 we found that judicious use of topical steroid can be beneficial when used with protective topical ganciclovir in patients undergoing corneal transplantation with diagnosed CMV endotheliitis. The present study highlighted the GC treatment, alone or in combination with antiviral therapy, delayed clearance of viral DNA through downregulation of cytokines, and elicited more profound viral lymphadenopathy.…”

Section: Discussionmentioning

confidence: 88%

Host Immune Response and Associated Clinical Features in a Primary Cytomegalovirus Eye Infection Model Using Anterior Chamber Inoculation

Gao

Peng

et al. 2022

Invest. Ophthalmol. Vis. Sci.

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Purpose To investigate the pathogenesis of cytomegalovirus (CMV)-associated anterior segment infection in immunocompetent hosts and evaluate the effects of ganciclovir and glucocorticoid treatment in management of the disease. Methods We used an inoculation model to reproduce CMV anterior segment infection in immunocompetent rats. Flow cytometry, cytokine analysis, histopathological sections, and quantitative polymerase chain reaction were performed to investigate the immune response after CMV infection. The effects of ganciclovir and glucocorticoid treatment were also assessed. Results Anterior chamber inoculation of CMV in rats provoked characteristic pathological features of human CMV anterior segment infection. The innate and adaptive immunity sequentially developed in an anterior segment after inoculation, and the elevation of intraocular pressure (IOP) was highly associated with ocular infiltration and inflammation. Early ocular immune response reduced virus DNA in the anterior segment and alleviated viral lymphadenopathy. Early intervention with ganciclovir enhanced the release of cytokines associated with T response and facilitated recruitment of NKT and T cells in drainage lymph nodes. Glucocorticoid treatment, alone or combined with ganciclovir, decreased elevation of IOP but also impeded DNA clearance. Conclusions The inoculation model reproduced characteristic pathological features of human CMV anterior segment infection. The use of glucocorticoid in current practice may hinder viral clearance, and ganciclovir therapy can assist cytokine expression to combat the virus.

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