Stimulation of accumulation of terpenoids by cell suspensions of Lavandula angustifolia following pre-treatment of parent callus

Banthorpe, D. V.; Bates, Margaret; Ireland, M. J.

doi:10.1016/0031-9422(95)00250-b

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…Significant levels of mono-and sesqui-terpenoids have been reported in callus cultures of Lavandula angustifolia Mill. [4]. Characterization of monoterpenoid volatiles has also been reported in callus cultures of Citrus sinensis (L.) [5a] and in cell suspension cultures of Agastache rugosa (Fisch.…”

mentioning

confidence: 99%

Cell Culture of Bursera Linanoe in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

Pavón-Reyes

Evangelista‐Lozano

Sepúlveda-Jiménez

et al. 2017

Natural Product Communications

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Bursera linanoe cell suspension cultures were initiated from callus grown in Murashige and Skoog medium supplemented with naphthalene acetic acid (3.0 mg L-1) and 6-benzylaminopurine (0.5 mg L-1). In flasks, B. linanoe cell cultures grew over a 9 day period, reaching a maximum biomass of 11.16 g DW L-1. Throughout the growth phase, cell viability was constant at 60-70%. In contrast, B. linanoe cells growing in a bioreactor achieved a maximum biomass of 22.26 g DW L-1 (after 7 days), and cell viability was constant at 75-85%. Production of linalool and linalyl acetate in the bioreactor (3.02 and 2.40 mg g-1 DW, respectively) was significantly greater than that achieved from cells in flask cultures (1.05 and 0.97 mg g-1 DW, respectively). B. linanoe cell suspension culture has potential as an alternative method for the production of essential oils.

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mentioning

confidence: 99%

Cell Culture of Bursera Linanoe in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

Pavón-Reyes

Evangelista‐Lozano

Sepúlveda-Jiménez

et al. 2017

Natural Product Communications

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“…Production of monoterpenes (i.e., limonene, linalool among others) in callus tissues and cell suspensions of Perilla frutescens paved the way to their large scale production (Sahai 1994). Significant levels of mono-and sesqui-terpenoids have also been reported in callus cultures of Lavandula angustifolia (Banthorpe et al 1995). Characterization of volatile flavour compounds has been reported in callus cultures of Citrus sinensis (Niedz et al 1997) and in cell suspension cultures of Korean mint Agastache rugosa (Kim et al 2001).…”

Section: Introductionmentioning

confidence: 90%

“…Thus plant cell cultures remain a favored methodology for biotechnological production of these natural compounds. Characterization of volatile flavour compounds and their large scale production using callus and suspension cultures of several plants including Perilla frutescens, Lavandula angustifolia, Oryza sativa and Agastache rugosa have been reported (Nabeta et al 1983;Sahai 1994;Suvarnalatha et al 1994;Banthorpe et al 1995). …”

Section: Characterization Of Aroma Compounds/terpenoidsmentioning

confidence: 99%

Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

Grover

Yadav

Biswas

et al. 2011

Plant Cell Tiss Organ Cult

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Cell suspension cultures of Camellia sinensis were established in 250 ml shake flasks. Flasks contained 50 ml liquid medium of either Murashige and Skoog (MS), N/5 MS or Heller medium containing different levels of 6-benzyladenine (BA) (0.05-2 mg l -1 ), 2,4-dichlorophenoxyacetic acid (2,4-D) (1-10 mg l -1 ), and sucrose (10-50 g l -1 ). Moreover, the pH of the medium was varied from 5.2-6.2. In addition, cultures were subjected to light irradiation as well as to complete darkness. Following optimization of aroma and terpenoid extraction methods, cell cultures were analyzed for the volatile compounds using GC/MS. A total of 43 compounds were identified using the micro SDE apparatus. Among the major monoterpenoids obtained were a-terpineol and nerol. Moreover, other high aroma-value compounds, including 2-ethyl hexanol, benzyl alcohol, benzene acetaldehyde, nonanal and phenylethylalcohol were also detected. The highest levels of these compounds were obtained from cell suspension cultures grown in MS medium containing 5 mg l -1 2,4-D, 1 mg l -1 BA and 30 g l -1 sucrose at pH of 5.8 with incubation in complete darkness.

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“…Several authors have studied the effect of different factors on the essential oil constituents in plant tissue culture. Banthorpe et al (1995) established callus lines of Lavandula angustifolia and found negligible amount of these terpenoids accumulated in derived cell suspension. They also added that pretreatment of callus tissues by pulse-feeding with mevalonate had little effect on terpenoid accumulation, but the derived cell suspension stored monoterpenoids at concentration ca.…”

Section: Essential Oils Constituents Of Shoot-tip Callus Culturesmentioning

confidence: 99%

“…In this respect, the effect of some hormonal and nutritional factors on monoterpene synthesis in Rosemarinus officinalis and Cnidium officinale callus cultures were investigated (Tawfik et al, 1992, andShin andPark, 1994). Accumulation of terpenoids by cell suspensions of Lavandula angustifolia was studied by Banthorpe et al (1995) Different authors used plant tissue culture techniques for production of essential oil and to compare between the in vitro cultures and the corresponding plants. In this context, production of monoterpene by tissue cultures of several Mentha species was reported by Charlwood and Charlwood, (1983).…”

Section: Introductionmentioning

confidence: 99%

EFFECT OF GROWTH REGULATORS ON ESSENTIAL OILS COMPOSITION OF Lavandula officinalis TISSUE CULTURES.

Rady

Youssef²

2002

Journal of Soil Sciences and Agricultural Engineering

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Shoot tips and leaves from Lavandula officinalis L. of mature greenhouse-grown plants were inoculated aseptically on callus initiation medium. Callus cultures were subcultured on MS-media supplemented with different growth regulators. The influence of growth regulators (2,4-D, BA, NAA and kinetin) on composition of the volatile compounds has been analyzed individually by gas chromatography (GC) for both types of callus tissues as well as the chemical analysis of in vitro produced plant leaves in comparison with the intact mature plants. Results obtained indicated that application of BA enhanced greatly the growth of shoot-tips derived callus which accounted about two times of that shown in MS-free-hormone medium, while application of kinetin is the most suitable regulating substance for enhanced leaves derived callus growth. Results also indicated that no essential oils were synthesized in shoot-tips and leaves derived callus originated from medium free from any growth regulators, while application of all growth regulators investigated enhanced markedly the biosynthesis of essential oils, which varied greatly at different extents. However, comparison of components, content of the oils and chemical composition between in vitro produced plants with those of leaves from plants growing in their natural habitat revealed that an increase in essential oil concentration accounted by 17% in in vitro plant and there is a profound differences in the composition of essential oils, but no considerable differences was observed in chemical composition.

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Stimulation of accumulation of terpenoids by cell suspensions of Lavandula angustifolia following pre-treatment of parent callus

Cited by 9 publications

References 18 publications

Cell Culture of Bursera Linanoe in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

Cell Culture of Bursera Linanoe in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

Production of monoterpenoids and aroma compounds from cell suspension cultures of Camellia sinensis

EFFECT OF GROWTH REGULATORS ON ESSENTIAL OILS COMPOSITION OF Lavandula officinalis TISSUE CULTURES.

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