Stimulation of Suicidal Erythrocyte Death by Sulforaphane

Alzoubi, Kousi; Calabrò, Salvatrice; Faggio, Caterina; Läng, Florian

doi:10.1111/bcpt.12309

Cited by 14 publications

(3 citation statements)

References 103 publications

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“…Approximately 10-25% of many liver proteins, including actin, tubulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), are sulfhydrated under physiological conditions. A similar effect of sulforaphane on RBC was observed in a model of eryptosis, a suicidal cell death characterized by cell shrinkage and phosphatidylserine translocation to the erythrocyte surface (Alzoubi et al, 2014). Persulfidation (a synonym for sulfhydration) of proteins requires attack of H 2 S to an oxidized side chain (e.g., a sulfenic acid or disulfide).…”

Section: Biochemistry and Molecular Interactions Of Oscmentioning

confidence: 61%

Organosulfur Compounds as Nutraceuticals

Гончаров¹,

Orekhov²,

Voitenko³

et al. 2016

Nutraceuticals

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Section: Biochemistry and Molecular Interactions Of Oscmentioning

confidence: 61%

Organosulfur Compounds as Nutraceuticals

Гончаров¹,

Orekhov²,

Voitenko³

et al. 2016

Nutraceuticals

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“…In this present study, we reviewed the most recent studies on the biological and pharmacological activities of SFN (Table 1(Tab. 1)) (References in Table 1: Pal and Konkimalla, 2016[34]; Zhao et al, 2016[55]; Wu et al, 2016[52]; Sasaki et al, 2016[39]; Jiang et al, 2016[17]; Hernández-Rabaza et al, 2016[15]; Sikdar et al, 2016[45]; Li et al, 2016[28]; Thaler et al, 2016[48]; Lan et al, 2016[23]; Shehatou and Suddek, 2016[42]; Townsend and Johnson, 2016[49]; Qi et al, 2016[37]; Abbas et al, 2016[1]; Kikuchi et al, 2015[21]; Atwell et al, 2015[6]; Ma et al, 2015[30]; Kim et al, 2015[22]; Wang et al, 2015[50]; Lubecka-Pietruszewska et al, 2015[29]; Brown et al, 2015[7]; Carrasco-Pozo et al, 2015[8]; Ambrecht et al, 2015[3]; Lavich et al, 2015[24]; Waston et al, 2015[51]; Shirai et al, 2015[43]; Prasad and Mishra, 2015[36]; Li et al, 2015[27]; Cipolla et al, 2015[9]; Angeloni et al, 2015[5]; Oguz et al, 2015[33]; Noh et al, 2015[32]; Shang et al, 2015[41]; Horwacik et al, 2015[16]; Shokeir et al, 2015[44]; Alzoubi et al, 2015[2]; Gabriel et al, 2015[13]; Kee et al, 2015[20]; Rizzo et al, 2014[38]; Pan et al, 2014[35]; Sayed et al, 2014[40]; Singh et al, 2014[46]; Maeda et al, 2014[31]; Zhang et al, 2014[54]; Lee et al, 2014[25]; Fimognari et al, 2014[12]; Jo et al, 2014[18]). …”

mentioning

confidence: 99%

Current potential health benefits of sulforaphane

Kim

Park

2016

EXCLI Journal; 15:Doc571; ISSN 1611-2156

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“…Eryptosis is stimulated by a large number of xenobiotics including the natural substances gambogic acid , tannic acid , withaferin , tanshinone , thymoquinone , ursolic acid , honokiol , saponin and apigenin . Moreover, eryptosis parallels several clinical conditions , such as dehydration , fever , sepsis , haemolytic–uraemic syndrome , end‐stage renal disease , hepatic failure , malaria , sickle cell disease , thalassaemia , Wilson's disease , iron deficiency , malignancy , metabolic syndrome and diabetes .…”

mentioning

confidence: 99%

Enhanced Eryptosis Following Juglone Exposure

Calabrò

Alzoubi

Bissinger

et al. 2014

Basic Clin Pharma Tox

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Juglone, a quinone isolated from Juglans mandshurica Maxim, has previously been shown to be effective against malignancy. The effect is at least partially due to stimulation of suicidal death or apoptosis of tumour cells. On the other hand, juglone has been shown to counteract apoptosis, for example, of neurons. In analogy to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and breakdown of phosphatidylserine asymmetry of the cell membrane with phosphatidylserine exposure at the erythrocyte surface. Stimulators of eryptosis include increase in cytosolic Ca 2+ activity [(Ca 2+ ) i ]. This study explored whether juglone stimulates eryptosis. To this end, erythrocyte volume was estimated from forward scatter, phosphatidylserine exposure at the erythrocyte surface from FITC annexin V binding, ceramide abundance from binding of fluorescent antibodies in flow cytometry and cytosolic ATP with a luciferin-luciferase-based assay. As a result, a 24-hr exposure of human erythrocytes to juglone (5 lM) significantly decreased erythrocyte forward scatter. Juglone (1-5 lM) significantly increased the percentage of annexin V binding cells. Juglone (5 lM) significantly increased ceramide abundance at the erythrocyte surface and decreased erythrocyte ATP concentration. The effect of juglone (10 lM) on annexin V binding was slightly but significantly blunted by removal of extracellular Ca 2+ and by addition of protein kinase C (PKC) inhibitor staurosporine (1 lM). In conclusion, juglone stimulates suicidal erythrocyte death or eryptosis at least in part by upregulation of ceramide abundance, energy depletion and activation of PKC.Juglone, a quinone isolated from Juglans mandshurica Maxim [1], has been shown to counteract malignancy [2]. The antitumour effects of juglone are at least partially due to stimulation of tumour cell apoptosis [1,[3][4][5][6][7]. On the other hand, juglone may inhibit neuronal cell death [8] and counteract vascular injury [9]. Mechanisms contributing to the proapoptotic effect of juglone include mitochondrial depolarization, oxidative stress and caspase activation [1,3,7,[10][11][12][13][14]. Moreover, juglone is effective by modifying gene expression [1,6]. Juglone has further been shown to be effective through downregulation of phosphoinositol 3-kinase (PI3K) [5] and inhibition of the peptidyl-prolyl isomerase Pin1 [8,9,[15][16][17][18][19][20][21][22][23].In spite of lacking mitochondria and nuclei, erythrocytes may enter an apoptosis-like suicidal death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with translocation of phosphatidylserine from the intracellular face of the cell membrane to the cell surface [24] [31,106,107].This study explored whether juglone stimulates eryptosis. To this end, the effect of juglone on cell volume, phosphatidylserine abundance at the cell surface, ceramide abundance and ATP content was determined in human erythrocytes. Materials and MethodsErythrocytes, solut...

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Stimulation of Suicidal Erythrocyte Death by Sulforaphane

Cited by 14 publications

References 103 publications

Organosulfur Compounds as Nutraceuticals

Organosulfur Compounds as Nutraceuticals

Current potential health benefits of sulforaphane

Enhanced Eryptosis Following Juglone Exposure

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