Stimuli-responsive polymeric materials for separation of biomolecules

Tan, Sinuo; Saito, Kei; Hearn, Milton T. W.

doi:10.1016/j.copbio.2018.02.011

Cited by 51 publications

(31 citation statements)

References 77 publications

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“…Stimuli-responsive polymers (SRPs), also known as "smart," "intelligent," or "environmentally responsive" polymers, can undergo alterations in their physical morphology and/or chemical properties under the influence of an external stimulus [1][2][3]. The thermo-responsive polymer (TRP), poly(N-isopropylacrylamide) (PNIPAAm), is one of the most well-investigated categories of SRPs with the ability to rapidly respond to small changes Article Related Abbreviations: BET, Brunauer-Emmett-Teller; bhLF, bovine holo-lactoferrin; bhTF, bovine holo-transferrin; LCST, lower critical solution temperatures; PNIPAAm, poly(Nisopropylacrylamide); SRP, stimuli-responsive polymer; TRP, thermo-responsive polymer in its surrounding environment.…”

Section: Introductionmentioning

confidence: 99%

“…For practical biotechnological applications, in many cases, the need arises to immobilize the SRPs onto a solid support media, such as silica-based materials [4][5][6][7][8][9] or polymeric support materials (e.g., porous polystyrene [10,11], Sepharose [12][13][14], or cellulose [15]). Such SRP-based materials have found application in the separation field because the "capture and release" of target molecules can be simply controlled by the temperature without the need to change the mobile phase composition to affect elution [1,2,16,17]. Low concentration buffer solutions or only water can be utilized as the mobile phase instead of employing gradients of organic solvents or high salt concentrations [1,2,5].…”

Section: Introductionmentioning

confidence: 99%

“…Such SRP-based materials have found application in the separation field because the "capture and release" of target molecules can be simply controlled by the temperature without the need to change the mobile phase composition to affect elution [1,2,16,17]. Low concentration buffer solutions or only water can be utilized as the mobile phase instead of employing gradients of organic solvents or high salt concentrations [1,2,5]. Due to the potential of these functional materials, thermo-responsive polymeric materials are being actively examined for the separation and purification of high-value biomolecules such as therapeutic proteins from their crude feedstocks [1,2,[16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…Low concentration buffer solutions or only water can be utilized as the mobile phase instead of employing gradients of organic solvents or high salt concentrations [1,2,5]. Due to the potential of these functional materials, thermo-responsive polymeric materials are being actively examined for the separation and purification of high-value biomolecules such as therapeutic proteins from their crude feedstocks [1,2,[16][17][18][19][20]. Despite this potential in the field of bio-separations with these functional materials to respond to an external stimulus, knowledge related to the underlying mechanism of interactions between biomolecules and the stimuliresponsive surface is still limited.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isothermal modelling of protein adsorption to thermo‐responsive polymer grafted Sepharose Fast Flow sorbents

Tan

Saito

Hearn

2021

J of Separation Science

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In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir-Freundlich, Temkin and Brunauer-Emmett-Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N-isopropylacrylamide)-based thermo-responsive copolymer-grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo-lactoferrin and bovine holotransferrin at 20 and 50 • C. The experimental data for bovine holo-lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1-40 mg/mL at both 20 and 50 • C. Similar analysis of the binding of the homologous protein, bovine holotransferrin, to the same thermo-responsive copolymer-grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isothermal modelling of protein adsorption to thermo‐responsive polymer grafted Sepharose Fast Flow sorbents

Tan

Saito

Hearn

2021

J of Separation Science

Self Cite

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show abstract

“…Stimuli‐responsive polymers, a kind of “smart” polymer, could respond to external stimuli (for example, pH, temperature, ionic strength, or light) with predictable and sharp changes in properties, which have attracted significant research interest in the fields of switchable pores/surfaces fabrication, controlled drug delivery and biomedical imaging/diagnostics . In recent years, the application of “smart” polymers have been extended to analytical separation . Bai et al prepared a pH‐responsive poly‐(acrylic acid‐ co ‐hydrazide) polymer as homogeneous enrichment system for efficient N ‐glycoprotein enrichment.…”

Section: Introductionmentioning

confidence: 99%

A Temperature‐Responsive Boronate Core Cross‐Linked Star (CCS) Polymer for Fast and Highly Efficient Enrichment of Glycoproteins

Chen

Tong

Dong

et al. 2019

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Fast and highly efficient enrichment and separation of glycoproteins is essential in many biological applications, but the lack of materials with high capture capacity, fast, and efficient enrichment/separation makes it a challenge. Here, a temperature‐responsive core cross‐linked star (CCS) polymer with boronate affinity is reported for fast and efficient enriching and separating of glycoproteins from biological samples. The temperature‐responsive CCS polymers containing boronic acid in its polymeric arms and poly(N‐isopropyl acrylamide) in its cross‐linked core are prepared using reversible addition‐fragmentation chain transfer polymerization via an “arm‐first” methodology. The soluble boronate polymeric arms of the CCS polymers provide a homogeneous reaction system and facilitate interactions between boronic acid and glycoproteins, which leads to a fast binding/desorption speed and high capture capacity. Maximum binding capacity of the prepared CCS polymer for horseradish peroxidase is determined to be 210 mg g−1, which can be achieved within 20 min. More interestingly, the temperature‐responsive CCS polymers exhibit rapid reversible thermal‐induced volume phase transition by increasing the temperature from 15 to 30 °C, resulting in a facile and convenient sample collection and recovery for the target glycoproteins. Finally, the temperature‐responsive CCS polymer is successfully applied to enrichment of low abundant glycoproteins.

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Biomolecule‐responsive polymers and their bio‐applications

Xiong,

Li,

Qing

2024

Interdisciplinary Materials

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Precise recognition and specific interactions between biomolecules are key prerequisites for ensuring the performance of all actives within living organisms. The convergence of biomolecular recognition systems into synthetic materials could endow the materials with high specificity and biological sensitivity; this, in turn, enables precise drug release, monitoring or detection of important biomolecules, and cell manipulation through targeted capture or release of specific biomolecules. Meanwhile, from the perspective of materials science, the application of conventional polymers in practical biological systems poses several challenges, such as low responsiveness and sensitivity, inadequate targetability, insufficient anti‐interference capacities, and unsatisfactory biocompatibility. These problems could be partly attributed to the polymers' weak discrimination abilities toward target biomolecules in the presence of interfering substances with high abundance. In particular, the proposition of “precision medicine” project raises higher demands for the design of biomaterials in terms of their precision and targetability. Therefore, there is an urgent demand for the development of new‐generation biomaterials with precise recognition and sensitive responsiveness comparable to biomacromolecules. This promotes a new research direction of biomolecule‐responsive polymers and their diverse applications. This review focuses on the origin and construction of biomolecule‐responsive polymers, as well as their attractive applications in drug delivery systems, bio‐detection, bio‐sensing, separation, and enrichment, as well as regulating cell adhesion.

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Stimuli-responsive polymeric materials for separation of biomolecules

Cited by 51 publications

References 77 publications

Isothermal modelling of protein adsorption to thermo‐responsive polymer grafted Sepharose Fast Flow sorbents

Isothermal modelling of protein adsorption to thermo‐responsive polymer grafted Sepharose Fast Flow sorbents

A Temperature‐Responsive Boronate Core Cross‐Linked Star (CCS) Polymer for Fast and Highly Efficient Enrichment of Glycoproteins

Biomolecule‐responsive polymers and their bio‐applications

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