In this work, one-pot green synthesis of a novel kind of water-compatible fluorescent molecularly imprinted polymer nanoparticle for selective optosensing of paracetamol was developed via macromolecular assembly of an amphiphilic fluorescent copolymer and in situ photo-cross-linking. Amphiphilic photo-cross-linkable and fluorescent copolymers containing carbazole groups were synthesized and could coassemble with paracetamol (PCM, template molecule) and photoinitiator in aqueous solution. The obtained photo-cross-linkable fluorescent nanoparticles were then cross-linked triggered by UV-irradiation, generating the hydrophilic and fluorescent molecularly imprinted polymer nanoparticles (FMIP nanoparticles) in aqueous media. The whole procedure was carried out at mild working condition, which is facile, green, and energy-saving. The resulting FMIP nanoparticles showed high selectivity toward PCM and obvious fluorescence quenching, induced by template-binding, in water and were a kind of efficient fluorescent chemosensor for the sensing of PCM. A wide linear range over PCM concentration from 4 to 1000 μM with a detection limit of 1.0 μM has been demonstrated using FMIP nanoparticles as chemosensor. Moreover, a rapid response of less than 2 min has been demonstrated. Finally, such a chemosensor based on FMIP nanoparticles was also successfully employed for the detection of PCM in commercial PCM tablets as well as urine samples.

Fast and highly efficient enrichment and separation of glycoproteins is essential in many biological applications, but the lack of materials with high capture capacity, fast, and efficient enrichment/separation makes it a challenge. Here, a temperature‐responsive core cross‐linked star (CCS) polymer with boronate affinity is reported for fast and efficient enriching and separating of glycoproteins from biological samples. The temperature‐responsive CCS polymers containing boronic acid in its polymeric arms and poly(N‐isopropyl acrylamide) in its cross‐linked core are prepared using reversible addition‐fragmentation chain transfer polymerization via an “arm‐first” methodology. The soluble boronate polymeric arms of the CCS polymers provide a homogeneous reaction system and facilitate interactions between boronic acid and glycoproteins, which leads to a fast binding/desorption speed and high capture capacity. Maximum binding capacity of the prepared CCS polymer for horseradish peroxidase is determined to be 210 mg g−1, which can be achieved within 20 min. More interestingly, the temperature‐responsive CCS polymers exhibit rapid reversible thermal‐induced volume phase transition by increasing the temperature from 15 to 30 °C, resulting in a facile and convenient sample collection and recovery for the target glycoproteins. Finally, the temperature‐responsive CCS polymer is successfully applied to enrichment of low abundant glycoproteins.

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