Storage conditions do not have detrimental effect on allograft collagen or scaffold performance

Abreu, Eduardo; Palmer, Matthew P.; Murray, Martha M.

doi:10.1007/s10561-009-9138-z

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…Dorlot et al (1980) found that frozen storage of canine anterior cruciate ligaments resulted in decreased elasticity, whereas Matthews and Ellis (1968) found the opposite to be true in a study on cat extensor communis and lateralis tendons. Viidik and Lewin (1966), Van Brocklin and Ellis (1965), and Abreu et al (2009) found no change in the elastic properties of rabbit anterior cruciate ligaments, human extensor digitorum tendons, and rat tail tendons, respectively, after freezing. Tarlton and Webster (2000) reported that storage at −20°C had no effect on the biomechanical strength of the suspensory tissue in bovine claws, but the details of this study have not been published.…”

Section: Technical Notementioning

confidence: 94%

Technical note: Effects of frozen storage on the mechanical properties of the suspensory tissue in the bovine claw

Boettcher¹,

Knudsen

Andersen

et al. 2014

Journal of Dairy Science

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It is proposed that a softening of the suspensory tissue in the claw is involved in the development of lameness and claw lesions in cattle. A relatively small amount of research has been carried out to verify this theory. Research in this area would be simplified if mechanical testing of the suspensory tissue could be performed on frozen and stored specimens. The current study tested whether freezing of the specimens changes the suspensory tissues' mechanical properties. Limbs from 3 freshly slaughtered Danish Holstein dairy cows and 6 nonpregnant Angus heifers, without clinical signs of lameness, were allocated to 1 of 2 treatments (frozen or nonfrozen) in such a way that each cow was represented in each treatment group with a frozen limb and a corresponding nonfrozen limb (i.e., frozen left front, fresh right front, and so on). The frozen limbs were kept at -18°C for a week before processing and the nonfrozen limbs were processed within 2h of slaughter. Two samples measuring 8 × 8 mm were cut from the abaxial side of each claw in such a way that the sample included the horn of the abaxial wall, pedal bone, and the interposed corium. The samples were kept on ice until being mounted in a large deformation rheometer with an extension testing frame, fixed by the horn and the pedal bone, and loaded to failure. During deformation force and displacement data were recorded, from which corresponding stress and strain were calculated. Young's modulus (a measure of tissue elasticity or stiffness) and a measure of physiological support (PS; force needed to displace the sample 1mm) were calculated from the data. The response variables, Young's modulus and PS, were analyzed separately by a mixed model. The explanatory variables were treatment (frozen or nonfrozen), limb (front or back), claw (medial or lateral), position of the sample (dorsal or palmar-plantar), and group (Angus or Holstein). Interactions between group and treatment and between limb, claw, and sample position were included in the model. Cow identity was included as a random effect. Model reduction was performed by stepwise backward elimination, until all remaining terms were significant at the 5% level or less. Freezing had no effect on the elasticity of the suspensory apparatus or on PS. However, PS was affected by limb (hind legs had higher PS values than front) and the position of the sample (palmar-plantar samples had higher PS values than dorsal). The Angus group had higher PS values than the Holstein group, but the groups differed in age, parity, body weight, lactation, housing, and management, as well as in breed; therefore, further studies are needed to investigate these effects. The results indicate that mechanical testing of bovine claw suspensory tissue can be performed on specimens that have been frozen, thus aiding research in the mechanical aspect of bovine lameness and claw lesions.

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Section: Technical Notementioning

confidence: 94%

Technical note: Effects of frozen storage on the mechanical properties of the suspensory tissue in the bovine claw

Boettcher¹,

Knudsen

Andersen

et al. 2014

Journal of Dairy Science

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“…The extracellular matrix-based scaffold (MIACH, Children’s Hospital Boston) was manufactured in our laboratory as previously described [11]). In brief, bovine connective tissue was solubilized and digested with pepsin.…”

Section: Methodsmentioning

confidence: 99%

Safety of Intra-Articular Use of Atelocollagen for Enhanced Tissue Repair

Magarian¹,

Vavken²,

Connolly³

et al. 2012

TOORTHJ

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Collagen is an important biomaterial in intra-articular tissue engineering, but there are unanswered questions about its safety. We hypothesize that the addition of type-I-collagen for primary repair of the Anterior Cruciate Ligament (ACL) might result in a local and systemic reaction in a porcine model after 15 weeks as demonstrated by joint effusion, synovial thickening, elevated intraarticular and systemic leukocyte counts. Further, this reaction might be aggravated by the addition of a platelet concentrate. Eighteen porcine ACLs were transected and repaired with either sutures (n=6), a collagen sponge (n=6), or a collagen-platelet-composite (CPC; n=6). Twelve intact contralateral knees served as controls (n=12). No significant synovial thickening or joint effusion was seen in the collagen-treated knees. Synovial fluid leukocyte counts showed no significant differences between surgically treated and intact knees, and no differences were seen in leukocyte counts of the peripheral blood. The addition of a platelet concentrate to the knee joint resulted in lower serum levels of IL-1β, but serum levels of TNF-α were not significantly different between groups. In conclusion, the presence of collagen, with or without added platelets, did not increase the local or systemic inflammatory reactions following surgery, suggesting that Type I collagen is safe to use in the knee joint.

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Safety of the Bio-enhanced Repair

Magarian

Murray

2013

The ACL Handbook

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Storage conditions do not have detrimental effect on allograft collagen or scaffold performance

Cited by 4 publications

References 30 publications

Technical note: Effects of frozen storage on the mechanical properties of the suspensory tissue in the bovine claw

Technical note: Effects of frozen storage on the mechanical properties of the suspensory tissue in the bovine claw

Safety of Intra-Articular Use of Atelocollagen for Enhanced Tissue Repair

Safety of the Bio-enhanced Repair

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