Strain specific motility patterns and surface adhesion of virulent and probiotic Escherichia coli

Abstract

Bacterial motility provides the ability for bacterial dissemination and surface exploration, apart from a choice between surface colonisation and further motion. In this study, we characterised the movement trajectories of pathogenic and probiotic Escherichia coli strains (ATCC43890 and M17, respectively) at the landing stage (i.e., leaving the bulk and approaching the surface) and its correlation with adhesion patterns and efficiency. A poorly motile strain JM109 was used as a control. Using specially designed and manufactured microfluidic chambers, we found that the motion behaviour near surfaces drastically varied between the strains, correlating with adhesion patterns. We consider two bacterial strategies for effective surface colonisation: horizontal and vertical, based on the obtained results. The horizontal strategy demonstrated by the M17 strain is characterised by collective directed movements within the horizontal layer during a relatively long period and non-uniform adhesion patterns, suggesting co-dependence of bacteria in the course of adhesion. The vertical strategy demonstrated by the pathogenic ATCC43890 strain implies the individual movement of bacteria mainly in the vertical direction, a faster transition from bulk to near-surface swimming, and independent bacterial behaviour during adhesion, providing a uniform distribution over the surface.

Bacterial proton motive force as an unprecedented target to control antimicrobial resistance

“…Furthermore, H + homeostasis is the fundamental premise of all life activities as enzymes playing catalytic roles require suitable pH conditions. Also, the rotation of bacterial flagella relies heavily on the energy released from ATP hydrolysis and participates in many physiological activities such as motion, surface adherence, biofilm formation, and host tissue colonization 35 . Altogether, stable PMF should be ensured for maintaining healthy bacterial activities.…”

Norepinephrine Effects on Uropathogenic Strains Virulence

“…To study the motility, bacteria were seeded in semi-liquid agar (0.5%) by puncturing the medium. The diameter of the grown colonies was measured in 24 h and 48 h of culturing (37 • C) [6].…”

“…The presence of a certain set of pathogenicity factors helps bacteria to colonize and spread from the initial site of infection. Thus, uropathogenic strains of E. coli are characterized by virulence factors such as fimbrial adhesins, cytotoxic necrotic factor type 1 and 2, hemolysins [4,5], high motility, and the capacity for biofilm formation [6,7].…”

Efficacy evaluation of anti-DEC-IgY against antibiotic-resistant diarrhoeagenic Escherichia coli

“…Abdulkadieva et al . [26] studied two different E. coli strains and found distinct horizontal and vertical motility patterns that may influence the adhesion efficiency of motile strains [26].…”

“…Studies have investigated the relationship between motility patterns and adhesion efficiency. Abdulkadieva et al [26] studied two different E. coli strains and found distinct horizontal and vertical motility patterns that may influence the adhesion efficiency of motile strains [26]. In the context of Salmonella, et al (2021) evaluated the inhibitory potency of IgY antibodies.…”