Strain-specific serological techniques for the identification of <i>Rhizobium meliloti</i> in commercial alfalfa inoculants

Olsen, P. E.; Rice, W. A.; Stemke, Gerald W.; Page, William

doi:10.1139/m83-037

Cited by 36 publications

(16 citation statements)

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“…The nodules were counted and their occupancy by S. meliloti strain NRG-34 was determined by enzyme-linked immunosorbent assay (ELISA) (Olsen et al 1983). Hay DM was determined in September 1991 and in June and September 1992 and 1993 by harvesting plants from two rows of each plot (0.6 m × 6 m) with a flail plot harvester (Thompson 1972).…”

Section: Methodsmentioning

confidence: 99%

Field evaluation of dual inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaii

Rice¹,

Lupwayi²,

Olsen³

et al. 2000

Can. J. Plant Sci.

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Field evaluation of dual inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaii. Can. J. Plant Sci. 80: [303][304][305][306][307][308]. Field experiments were conducted at five sites to evaluate the effects of dual inoculation of alfalfa (Medicago sativa L.) with Sinorhizobium meliloti and Penicillium bilaii, a phosphorus-solubilizing fungus, on the yield and quality of alfalfa hay. Dual inoculation with S. meliloti strain NRG-34 and P. bilaii strain PB-50 increased nodule number and occupancy, but resulted in only small increases in hay yield and total nitrogen and phosphorus concentrations of hay compared with inoculation with S. meliloti alone. There was little response of alfalfa to phosphorus fertilizer at the sites, and this may partly explain the small effects of dual inoculation of the two microorganisms. There were no significant differences in nodulation, hay yield or hay nutrient concentrations between alfalfa inoculated with separate NRG-34 and PB-50 inoculant products and that inoculated with a co-cultured inoculant. This similarity in response supports use of a single effective delivery system for the two different rhizosphere microorganisms, which is of great practical relevance as producers are more likely to adopt technology that requires a single inoculation procedure than one requiring two separate inoculation procedures at planting time. meliloti, mais n'a donné lieu qu'à de légères augmentations du rendement en foin ainsi que des teneurs du foin en N et en P total, par comparaison avec l'inoculation du seul S. meliloti. Cela pourrait s'expliquer en partie par le peu de réponse de la luzerne à la fumure phosphorée manifesté aux emplacements des expériences. Il n'y avait pas de différences significatives relativement à la nodulation, au rendement en foin ou au contenu nutritionnel de la luzerne selon que l'ensemencement était fait avec les deux souches produites en cultures séparées ou en association. Cette similarité de réponse incite à opter pour un bon système d'ensemencement unique pour les deux micro-organismes, ce qui pour les producteurs aurait l'avantage pratique de pouvoir exé-cuter l'inoculation en une seule opération plutôt que de devoir le faire en deux opérations distinctes.

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Section: Methodsmentioning

confidence: 99%

Field evaluation of dual inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaii

Rice¹,

Lupwayi²,

Olsen³

et al. 2000

Can. J. Plant Sci.

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“…To measure the bacterial number, commonly known methods in microbiology are used; the traditional Plate Count methods, Most Problable Number (Woomer et al 1990), ELISA, and Immunoblot (Olsen and Rice, 1989;Olsen et al 1983;Rice and Olsen 1988).…”

Section: Regulation and Control Of Contamination Of Commercial Inoculmentioning

confidence: 99%

A Brief Historical Background

Bhattacharjee¹

2012

The Second Homeland: Polish Refugees in India

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An assessment of the current state of bacterial inoculants for contemporary agriculture in developed and developing countries is critically evaluated from the point of view of their actual status and future use. Special emphasis is given to two new concepts of inoculation, as yet unavailable commercially: (i) synthetic inoculants under development for plant-growth promoting bacteria (PGPB) (Bashan and Holguin, 1998), and (ii) inoculation by groups of associated bacteria.This review contains: A brief historical overview of bacterial inoculants; the rationale for plant inoculation with emphasis on developing countries and semiarid agriculture, and the concept and application of mixed inoculant; discussion of microbial formulation including optimization of carrier-compound characteristics, types of existing carriers for inoculants, traditional formulations, future trends in formulations using unconventional materials, encapsulated synthetic formulations, macro and micro formulations of alginate, encapsulation of beneficial bacteria using other materials, regulation and contamination of commercial inoculants, and examples of modem commercial bacterial inoculants; and a consideration of time constraints and application methods for bacterial inoculants, commercial production, marketing, and the prospects of inoculants in modern agriculture.® 1998 Elsevier Science Inc.

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“…Growth curves were obtained and doubling times (td) calculated for each culture (Drew, 1981). At the end of the incubation period, an ELISA (Olsen et al, 1983;Olsen and Rice, 1984) was run on each culture using non-strain-specific anti-Rhizobium meliloti polyclonal antibodies. Any culture testing negative was either plated several times, selecting single colony isolates until a pure culture of R. meliloti was obtained, or was discarded from further evaluation.…”

Section: Culture Growth At 10 O Cmentioning

confidence: 99%

“…Isolates NRG-34 and NRG-38 were identified as distinct strains, and a highly specific monoclonal antibody against NRG-34 was produced (Olsen et al, 1994). Isolate NRG-185 is a commercial R. meliloti inoculant strain for which a specific polyclonal antibody is available (Olsen et al , 1983). Alfalfa (cv.…”

Section: Fieldstudymentioning

confidence: 99%

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Symbiotic effectiveness of Rhizobium meliloti at low root temperature

1995

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Laboratory, growth chamber and field experiments were conducted to select among 226 isolates of Rhizobium meliloti for the ability to grow, nodulate alfalfa (Medicago sativa L.) and support N2-dependent plant growth between 9 ° and 12 o C. There was wide variation in the abilities of R. meliloti isolates to grow and form nodules at 10 °C. Culture doubling times (td) varied from 1 to 155 h, and the number of nodules formed on alfalfa in growth pouches in 2 weeks varied from 0 to 3.8 nodules per plant. Nodulation occurred at 9 °C, but there was no significant N2-dependent plant growth at this temperature. However, several isolates of R. meliloti had the ability to nodulate alfalfa and produce N2 -dependent growth at root temperatures between 10 o and 12 °C. Six isolates, including strain NRG-34, produced significantly more N2-dependent plant growth at 12 °C root temperature than did 14 other isolates tested. In field experiments, inoculation with strain NRG-34 resulted in greater nodule numbers, nodule weight, proportion of nodules occupied by the inoculant strain and plant weight than did inoculation with a commercial strain (NRG-185). These results permitted selection of a strain with better low-temperature competitive abilities than the currently available commercial strains.

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Strain-specific serological techniques for the identification of Rhizobium meliloti in commercial alfalfa inoculants

Cited by 36 publications

References 0 publications

Field evaluation of dual inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaii

Field evaluation of dual inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaii

A Brief Historical Background

Symbiotic effectiveness of Rhizobium meliloti at low root temperature

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