Strategy and Chromatographic Technology of Quality Control for Traditional Chinese Medicines

Abstract

The application of chromatography, a modern technology for highly efficient separation and analysis in the research of therapeutic basis matter and quality control of traditional chinese medicine (TCM), is summarized. A new strategy with metabonomics is put forward to achieve integral study on therapeutic basis matter and action mechanism of TCM, and to reveal and control the comprehensive quality of TCM. Modern chromatography and hyphenated techniques are one of the important technique platforms in quality control of TCM.

Simultaneous quantification of twelve active components in Yiqing granule by ultra‐performance liquid chromatography: application to quality control study

“…In the process, chromatographic methods such as high-performance liquid chromatography (HPLC), gas chromatography (GC), capillary electrophoresis (CE) and liquid chromatography-mass spectrometry (LC-MS) have often been used (Li et al, 2006;Liang et al, 2009). Ultra-performance liquid chromatography (UPLC) has earned special attention in pharmaceutical and biomedical analysis as soon as it came out in 2004 (Li et al, 2005;Wren and Tchlitcheff, 2006).…”

Recent analytical approaches in quality control of traditional Chinese medicines—A review

“…Since the introduction of serum pharmacology and serum pharmacochemistry to screen the bioactive compounds by Homma et al [28], this method has been used widely for the clarification of the possible therapeutic basis and action mechanism of TCMs, through comparison of the chemical profile of a TCM and its metabolites profile [29][30][31][32][33]. Zhang analyzed the chemical fingerprint and metabolic fingerprint of Salvia miltiorrhiza injection with HPLC-UV and HPLC-MS, and defined polyphenolic acids as the main therapeutic matter.…”

“…However, these biofingerprints are mainly studying the properties of absorption, distribution, metabolism and excretion (ADME) of TCMs [4,9], which cannot address directly the activity of the compounds, so a more direct method has been developed, namely the profile-effect study. This method concentrates on the correlation studies between chemical compounds (chemical fingerprint) and pharmacological data [29,146]. Li combined HPLC fingerprint analysis and bacteriostasis activity test for studying L. japonica.…”

Gel Permeation Chromatography Purification and Gas Chromatography-Mass Spectrometry Detection of Multi-Pesticide Residues in Traditional Chinese Medicine

“…The influence of TCM on health care system has been profound in Asia as well as in the West in recent years [1][2][3]. The concerning of contaminations of heavy metals [4][5][6] and pesticides [2,[6][7][8][9], which may be introduced during the cultivation, transportation, preparation and preservation, has been increased as the popularity of TCM enlarged.…”