Strategy of controlling the volumetric loading rate to promote hydrogen-production performance in a mesophilic-kitchen-waste fermentor and the microbial ecology analyses

Li, Shiue-Lin; Lin, Jie; Wang, Yu-Hsuan; Lee, Ze-Kun; Kuo, Shih-Chiang; Tseng, I-Cheng; Cheng, Sheng-Shung

doi:10.1016/j.biortech.2011.02.067

Cited by 30 publications

(7 citation statements)

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“…Bifidobacterium and Olsenella dominated in eleven of the 14 sorted sub-communities that correlated strongly positive to caproate and caprylate concentrations. Both genera produce lactate from carbohydrates, either after general amylolytic activity [47] or through the Bifidobacterium specific “bifid shunt” [48]. These two genera of lactic acid bacteria have been previously detected as major parts of microbial communities producing short-chain fatty acids [49–51].…”

Section: Discussionmentioning

confidence: 99%

Key sub-community dynamics of medium-chain carboxylate production

et al. 2019

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Background The carboxylate platform is a promising technology for substituting petrochemicals in the provision of specific platform chemicals and liquid fuels. It includes the chain elongation process that exploits reverse β–oxidation to elongate short-chain fatty acids and forms the more valuable medium-chain variants. The pH value influences this process through multiple mechanisms and is central to effective product formation. Its influence on the microbiome dynamics was investigated during anaerobic fermentation of maize silage by combining flow cytometric short interval monitoring, cell sorting and 16S rRNA gene amplicon sequencing. Results Caproate and caprylate titres of up to 6.12 g L −1 and 1.83 g L −1 , respectively, were achieved in a continuous stirred-tank reactor operated for 241 days. Caproate production was optimal at pH 5.5 and connected to lactate-based chain elongation, while caprylate production was optimal at pH 6.25 and linked to ethanol utilisation. Flow cytometry recorded 31 sub-communities with cell abundances varying over 89 time points. It revealed a highly dynamic community, whereas the sequencing analysis displayed a mostly unchanged core community. Eight key sub-communities were linked to caproate or caprylate production (r S > | ± 0.7|). Amongst other insights, sorting and subsequently sequencing these sub-communities revealed the central role of Bifidobacterium and Olsenella , two genera of lactic acid bacteria that drove chain elongation by providing additional lactate, serving as electron donor. Conclusions High-titre medium-chain fatty acid production in a well-established reactor design is possible using complex substrate without the addition of external electron donors. This will greatly ease scaling and profitable implementation of the process. The pH value influenced the substrate utilisation and product spectrum by shaping the microbial community. Flow cytometric single cell analysis enabled fast, short interval analysis of this community and was coupled with 16S rRNA gene amplicon sequencing to reveal the major role of lactate-producing bacteria. Electronic supplementary material The online version of this article (10.1186/s12934-019-1143-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Key sub-community dynamics of medium-chain carboxylate production

et al. 2019

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“…The dominant clones retrieved from a starch-fed hydrogen fermenter, a starch-peptone fed CSTR and a kitchen waste fermenter at low HRT were affiliated to Bifidobacterium sp., Lactobacillus plantarum and Olsenella genomosp (similar to Lactobacillus sp. ), respectively (Cheng et al 2008;Li et al 2010Li et al , 2011. These three facultative heterofermentative LAB can hydrolyze starch to produce mainly lactate and some trace of acetate, but no hydrogen, and were more abundant during the periods of highest carbohydrate removal efficiency and H 2 production, indicating their amylolytic activity (Cheng et al 2008;Li et al 2010Li et al , 2011.…”

Section: Substrate Hydrolysismentioning

confidence: 99%

“…), respectively (Cheng et al 2008;Li et al 2010Li et al , 2011. These three facultative heterofermentative LAB can hydrolyze starch to produce mainly lactate and some trace of acetate, but no hydrogen, and were more abundant during the periods of highest carbohydrate removal efficiency and H 2 production, indicating their amylolytic activity (Cheng et al 2008;Li et al 2010Li et al , 2011. In sludge from cattle manure compost incubated with cellobiose substrate, the functional bacterial consortium that could effectively hydrolyze cellobiose and produce hydrogen was composed of a cellobiose-hydrolyzing bacteria (Enterococcus saccharolyticus, from the order Lactobacillales, which harbors hydrolytic activity for a wide range of substrates) associated with an HPB (C. butyricum) (Adav et al 2009).…”

Section: Substrate Hydrolysismentioning

confidence: 99%

“…In kitchen waste fermenter, Desulfovibrio sp. was detected and suspected to be involved in lactate degradation (Li et al 2011) which involves hydrogen production (McInerney and Bryant 1981). Interestingly, the ability to efficiently produce or consume molecular hydrogen depending on the environmental conditions has been evidenced in Desulfovibrio: when sulfate concentrations are low, it can grow on lactate and evolve varying amounts of hydrogen, during the early phase of growth (Odom and Peck 1981).…”

Section: Hydrogen Consumption By Sulfate-reducing Bacteriamentioning

confidence: 99%

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Microbial ecology of fermentative hydrogen producing bioprocesses: useful insights for driving the ecosystem function

Cabrol

Marone

Tapia-Venegas

et al. 2017

FEMS Microbiology Reviews

247

103

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One of the most important biotechnological challenges is to develop environment friendly technologies to produce new sources of energy. Microbial production of biohydrogen through dark fermentation, by conversion of residual biomass, is an attractive solution for short-term development of bioH2 producing processes. Efficient biohydrogen production relies on complex mixed communities working in tight interaction. Species composition and functional traits are of crucial importance to maintain the ecosystem service. The analysis of microbial community revealed a wide phylogenetic diversity that contributes in different-and still mostly unclear-ways to hydrogen production. Bridging this gap of knowledge between microbial ecology features and ecosystem functionality is essential to optimize the bioprocess and develop strategies toward a maximization of the efficiency and stability of substrate conversion. The aim of this review is to provide a comprehensive overview of the most up-to-date biodata available and discuss the main microbial community features of biohydrogen engineered ecosystems, with a special emphasis on the crucial role of interactions and the relationships between species composition and ecosystem service. The elucidation of intricate relationships between community structure and ecosystem function would make possible to drive ecosystems toward an improved functionality on the basis of microbial ecology principles.

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“…5) and had a relative abundance of less than 0.56% of the sequences obtained from the microbial analysis. These OTUs were related to genus Olsenella, Bifidobacterium and Pelosinus, which have been reported in communities of fermentative reactors [32,39,40].…”

Section: Characterization Of the Microbial Communitymentioning

confidence: 98%