Streptococcus equi antigens adsorbed onto surface modified poly-ɛ-caprolactone microspheres induce humoral and cellular specific immune responses

Florindo, Helena F.; Pandit, S.; Gonçalves, Lídia; Alpar, H. Oya; Almeida, António J.

doi:10.1016/j.vaccine.2008.05.074

Cited by 40 publications

(16 citation statements)

References 45 publications

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“…9,10 Briefly, PCL was dissolved in 5 mL dichloromethane and emulsified by homogenization using an Ultra-Turrax T10 basic (IKA, Staufen, Germany) for 3 minutes with a 10% (w/w) poly(vinyl alcohol) solution, where the antibiotics were previously solubilized. The resulting (w/o) emulsion was added to 30 mL of 1.25% (w/w) poly(vinyl alcohol) solution and emulsified by homogenization using a Silverson Laboratory Mixer Emulsifier L5M (Silverson Machines Inc., Buckinghamshire, UK) for 7 minutes at maximum rotation speed.…”

Section: Preparation Of Antibiotic-loaded Pcl Microparticlesmentioning

confidence: 99%

“…The particle size distribution of plain and antibiotic-loaded PCL microparticles presented a monomodal population within the micrometer range, which is consistent with reports of polymeric microparticles prepared with the same method. 9,10 The negative charge from all formulations is attributed to the fact that PCL is negatively charged. 10 In addition, the encapsulation of daptomycin or vancomycin did not influence particle size distribution or surface charge (Table 1).…”

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confidence: 99%

“…9,10 The negative charge from all formulations is attributed to the fact that PCL is negatively charged. 10 In addition, the encapsulation of daptomycin or vancomycin did not influence particle size distribution or surface charge (Table 1). Furthermore, the differential scanning calorimetry (DSC) thermograms revealed that PCL in the microparticles maintained its crystalline state, whereas both antibiotics were amorphous (Figure 3).…”

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confidence: 99%

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Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms

Ferreira¹,

Bettencourt²,

Gonçalves³

et al. 2015

IJN

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The aim of the present study was to develop novel daptomycin-loaded poly-epsilon-caprolactone (PCL) microparticles with enhanced antibiofilm activity against mature biofilms of clinically relevant bacteria, methicillin-resistant Staphylococcus aureus (MRSA) and polysaccharide intercellular adhesin-positive Staphylococcus epidermidis . Daptomycin was encapsulated into PCL microparticles by a double emulsion-solvent evaporation method. For comparison purposes, formulations containing vancomycin were also prepared. Particle morphology, size distribution, encapsulation efficiency, surface charge, thermal behavior, and in vitro release were assessed. All formulations exhibited a spherical morphology, micrometer size, and negative surface charge. From a very early time stage, the released concentrations of daptomycin and vancomycin were higher than the minimal inhibitory concentration and continued so up to 72 hours. Daptomycin presented a sustained release profile with increasing concentrations of the drug being released up to 72 hours, whereas the release of vancomycin stabilized at 24 hours. The antibacterial activity of the microparticles was assessed by isothermal microcalorimetry against planktonic and sessile MRSA and S. epidermidis . Regarding planktonic bacteria, daptomycin-loaded PCL microparticles presented the highest antibacterial activity against both strains. Isothermal microcalorimetry also revealed that lower concentrations of daptomycin-loaded microparticles were required to completely inhibit the recovery of mature MRSA and S. epidermidis biofilms. Further characterization of the effect of daptomycin-loaded PCL microparticles on mature biofilms was performed by fluorescence in situ hybridization. Fluorescence in situ hybridization showed an important reduction in MRSA biofilm, whereas S. epidermidis biofilms, although inhibited, were not eradicated. In addition, an important attachment of the microparticles to MRSA and S. epidermidis biofilms was observed. Finally, all formulations proved to be biocompatible with both ISO compliant L929 fibroblasts and human MG63 osteoblast-like cells.

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Section: Preparation Of Antibiotic-loaded Pcl Microparticlesmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms

Ferreira¹,

Bettencourt²,

Gonçalves³

et al. 2015

IJN

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“…PCL nanospheres were prepared aseptically at room temperature by a modification of a double emulsion (w/o/w) solvent evaporation method previously reported [20]. In brief, polymer was dissolved in 6 ml DCM and emulsified by homogenisation using an Ultra-Turrax T25 (Janke & Kunkel, IKA-Labortechnik) for 3 min at 24,000 rpm, with a 10% (w/v) PVA or a 1% (w/v) GCS solution, which contained the S. equi antigens (10.0 mg) in the formulation of nanospheres loaded by encapsulation.…”

Section: Preparation Of Pcl Nanospheresmentioning

confidence: 99%

“…In a previous work, our group has shown that non-aggregated S. equi enzymatic extract protein-adsorbed PCL microspheres enhanced serum specific IgG, IgG1 and IgG2a antibody responses and elicited an elevated type I immune response (Th1) cytokines, even 300 days after a single subcutaneous dose administration [20].…”

Section: Introductionmentioning

confidence: 96%

The enhancement of the immune response against S. equi antigens through the intranasal administration of poly-ɛ-caprolactone-based nanoparticles

Florindo¹,

Pandit²,

Lacerda³

et al. 2009

Biomaterials

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Micro and Nanoparticle‐Based Delivery Systems for Vaccine Immunotherapy: An Immunological and Materials Perspective

Leleux

Roy

2012

Adv Healthcare Materials

175

148

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The development and widespread application of vaccines has been one of the most significant achievements of modern medicine. Vaccines have not only been instrumental in controlling and even eliminating life-threatening diseases like polio, measles, diphtheria, etc., but have also been immensely powerful in enhancing the worldwide outlook of public health over the past century. Despite these successes, there are still many complex disorders (e.g., cancer, HIV, and other emerging infectious diseases) for which effective preventative or therapeutic vaccines have been difficult to develop. This failure can be attributed primarily to our inability to precisely control and modulate the highly complex immune memory response, specifically the cellular response. Dominated by B and T cell maturation and function, the cellular response is primarily initiated by potent immunostimulators and antigens. Efficient and targeted delivery of these immunomodulatory and immunostimulatory molecules to appropriate cells is key to successful development of next generation vaccine formulations. Over the past decade, particulate carriers have emerged as an attractive means for enhancing the delivery efficacy and potency of vaccines and associated immunomodulatory molecules. Specifically, polymer-based micro and nanoparticles are being extensively studied for a wide variety of applications. In this review, we discuss the immunological fundamentals for developing effective vaccines and how materials and material properties can be exploited to improve these therapies. Particular emphasis is given to polymer-based particles and how the route of administration of particulate systems affects the phenotype and robustness of an immune response. Comparison of various strategies and recent advancements in the field are discussed along with insights into current limitations and future directions.

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Streptococcus equi antigens adsorbed onto surface modified poly-ɛ-caprolactone microspheres induce humoral and cellular specific immune responses

Cited by 40 publications

References 45 publications

Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms

Activity of daptomycin- and vancomycin-loaded poly-epsilon-caprolactone microparticles against mature staphylococcal biofilms

The enhancement of the immune response against S. equi antigens through the intranasal administration of poly-ɛ-caprolactone-based nanoparticles

Micro and Nanoparticle‐Based Delivery Systems for Vaccine Immunotherapy: An Immunological and Materials Perspective

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