Streptococcus zooepidemicus Meningitis and Bacteraemia

Ural, Onur; Tuncer, İnci; Dikici, Nebahat; Arıdoğan, Buket Cicioğlu

doi:10.1080/00365540310000076

Cited by 30 publications

(12 citation statements)

References 5 publications

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“…S. zooepidemicus opportunistically produces respiratory disease in situations involving viral infections, heat stress, or prolonged transportation (4). Select clones can be devastating pathogens in intensively housed dogs and guinea pigs and in humans following consumption of contaminated milk or cheese (5)(6)(7).…”

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confidence: 99%

Characterization and Protective Immunogenicity of the SzM Protein of Streptococcus zooepidemicus NC78 from a Clonal Outbreak of Equine Respiratory Disease

Velineni

Timoney

2013

Clin Vaccine Immunol

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Streptococcus zooepidemicus of Lancefield group C is a highly variable tonsillar and mucosal commensal that usually is associated with opportunistic infections of the respiratory tract of vertebrate hosts. More-virulent clones have caused epizootics of severe respiratory disease in dogs and horses. The virulence factors of these strains are poorly understood. The antiphagocytic protein SeM is a major virulence factor and protective antigen of Streptococcus equi, a clonal biovar of an ancestral S. zooepidemicus strain. Although the genome of S. zooepidemicus strain H70, an equine isolate, contains a partial homolog (szm) of sem, expression of the gene has not been documented. We have identified and characterized SzM from an encapsulated S. zooepidemicus strain from an epizootic of equine respiratory disease in New Caledonia. The SzM protein of strain NC78 (SzM NC78 ) has a predicted predominantly alpha-helical fibrillar structure with an LPSTG cell surface anchor motif and resistance to hot acid. A putative binding site for plasminogen is present in the B repeat region, the sequence of which shares homology with repeats of the plasminogen binding proteins of human group C and G streptococci. Equine plasminogen is activated in a dose-dependent manner by recombinant SzM NC78 . Only 23.20 and 25.46% DNA homology is shared with SeM proteins of S. equi strains CF32 and 4047, respectively, and homology ranges from 19.60 to 54.70% for SzM proteins of other S. zooepidemicus strains. As expected, SzM NC78 reacted with convalescent-phase sera from horses with respiratory disease associated with strains of S. zooepidemicus. SzM NC78 resembles SeM in binding equine fibrinogen and eliciting strong protective antibody responses in mice. Sera of vaccinated mice opsonized S. zooepidemicus strains NC78 and W60, the SzM protein of which shared partial amino acid homology with SzM NC78 . We conclude that SzM is a protective antigen of NC78; it was strongly reactive with serum antibodies from horses during recovery from S. zooepidemicus-associated respiratory disease.

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confidence: 99%

Characterization and Protective Immunogenicity of the SzM Protein of Streptococcus zooepidemicus NC78 from a Clonal Outbreak of Equine Respiratory Disease

Velineni

Timoney

2013

Clin Vaccine Immunol

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“…In China, it is an important swine pathogen, causing septicemia, meningitis, endocarditis and arthritis, resulting in significant economic losses worldwide [2], [3]. Humans may contract S. z ooepidemicus after consuming contaminated food or from close contact with infected animals [4]–[6]. In 1975, Sichuan province experienced a S. z ooepidemicus pandemic, resulting in the death of 300,000 pigs and great economic losses.…”

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confidence: 99%

Microarray Analysis of the Effect of Streptococcus equi subsp. zooepidemicus M-Like Protein in Infecting Porcine Pulmonary Alveolar Macrophage

Zhang

et al. 2012

PLoS ONE

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Streptococcus equi subsp. zooepidemicus (S. zooepidemicus), which belongs to Lancefield group C streptococci, is an important pathogen of domesticated species, causing septicemia, meningitis and mammitis. M-like protein (SzP) is an important virulence factor of S. zooepidemicus and contributes to bacterial infection and antiphagocytosis. To increase our knowledge of the mechanism of SzP in infection, we profiled the response of porcine pulmonary alveolar macrophage (PAM) to infection with S. zooepidemicus ATCC35246 wild strain (WD) and SzP-knockout strain (KO) using the Roche NimbleGen Porcine Genome Expression Array. We found SzP contributed to differential expression of 446 genes, with upregulation of 134 genes and downregulation of 312 genes. Gene Ontology category and KEGG pathway were analyzed for relationships among differentially expressed genes. These genes were represented in a variety of functional categories, including genes involved in immune response, regulation of chemokine production, signal transduction and regulation of apoptosis. The reliability of the data obtained from the microarray was verified by performing quantitative real-time PCR on 12 representative genes. The data will contribute to understanding of SzP mediated mechanisms of S. zooepidemicus pathogenesis.

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“…It is the most frequently isolated pyogen from equine nasal cavities, joints, lymph nodes and lungs and a primary cause of respiratory tract infections in foals and uterine infection in mares . It can be a devastating pathogen in intensively housed dogs, cats and guinea pigs and in man .…”

Section: Introductionmentioning

confidence: 99%

Capsular hyaluronic acid of equine isolates of Streptococcus zooepidemicus is upregulated at temperatures below 35°C

Velineni

Timoney

2014

Equine Veterinary Journal

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Sensitivity of capsule expression to temperature above 35°C but not HylC by the general population of equine S. zooepidemicus indicates that capsule is not required for extended colonisation nor for opportunist invasion. Instead, capsule production at lower than body temperature may reflect adaptation to life on skin and mucosal surfaces, where hyaluronic acid contributes to adhesion and resistance to desiccation. Pathogenicity of S. zooepidemicus following opportunist invasion is possibly dependent on factors other than capsule that may be co-regulated with HylC. The Summary is available in Chinese - see Supporting information.

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Streptococcus zooepidemicus Meningitis and Bacteraemia

Cited by 30 publications

References 5 publications

Characterization and Protective Immunogenicity of the SzM Protein of Streptococcus zooepidemicus NC78 from a Clonal Outbreak of Equine Respiratory Disease

Characterization and Protective Immunogenicity of the SzM Protein of Streptococcus zooepidemicus NC78 from a Clonal Outbreak of Equine Respiratory Disease

Microarray Analysis of the Effect of Streptococcus equi subsp. zooepidemicus M-Like Protein in Infecting Porcine Pulmonary Alveolar Macrophage

Capsular hyaluronic acid of equine isolates of Streptococcus zooepidemicus is upregulated at temperatures below 35°C

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