Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China

Abstract

The taxonomic position of an actinomycete isolated from radiation-polluted soil collected in Xinjiang Province, north-west China, was determined by using a polyphasic approach. The isolate, designated strain R97T, had chemical and morphological properties characteristic of streptomycetes. An almost-complete 16S rRNA gene sequence of the isolate was generated and compared with corresponding sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus Streptomyces but also showed that it represented a distinct phyletic line that was most closely, albeit loosely, associated with three other thermotolerant organisms, namely Streptomyces macrosporus NBRC 14748T, Streptomyces megasporus NBRC 14749T and Streptomyces thermolineatus NBRC 14750T. Strain R97T could be distinguished from these organisms based on a range of phenotypic properties. It is proposed that R97T (=CGMCC 4.3519T =DSM 41901T) be classified as the type strain of a novel species in the genus Streptomyces, Streptomyces radiopugnans sp. nov. The organism was shown to be resistant to 60Co gamma radiation at a dose of 15 kGy.

Biosystematics of alkaliphilic streptomycetes isolated from seven locations across a beach and dune sand system

“…These are interesting results as nearly all Streptomyces type strains grow over a restricted pH range and optimally at pH 7.0 (Mikami et al 1982), exceptions include the type strains of S. glauciniger (Huang et al 2004), S. jietaisiensis (He et al 2005), S. radiopugnans (Mao et al 2007), ''S. sannurensis'' (Hozzein et al 2008) and those of the thermophiles S. thermoalcalitolerans, S. thermocarboxydovorons, S. thermodenitrificans and S. thermovulgaris all of which grow over an extensive pH range (Kim et al 1999).…”

“…Mikami et al (1982) distinguished between alkaliphilic actinomycetes, which grew between pH 8.0 and 11.5, with optimal growth around pH 9.0-9.5, from alkaline-resistant actinomycetes which grew at high pH values but optimally at pH 7.0. Alkaliphilic and alkalitolerant streptomycetes are common in alkaline soils (Taber 1960;Basilio et al 2003;Antony-Babu et al 2007), but few such organisms have been formally described as novel Streptomyces species (Kim et al 1999;Huang et al 2004;He et al 2005;Hozzein et al 2008;Mao et al 2007). …”

Phylogenetic‐affiliation, antimicrobial potential and PKS gene sequence analysis of moderately halophilic Streptomyces sp. inhabiting an Indian saltpan

“…Streptomyces sp. JAJ06 differs from its closest relative S. radiopugnans [29] in their growth characteristics and inability to utilize inulin, mannitol and xylose.…”

Streptomyces verrucosisporus sp. nov., isolated from marine sediments

“…In addition, the presence of lysophosphatidylethanolamine in the phospholipid profile was distinctive from Streptomyces fenghuangensis NRRL B-24801 T and related type strains ( Fig. S1; Yousif et al, 2015;Ray et al, 2014;Mao et al, 2007). According to Ray et al (2014), growth of Streptomyces barkulensis RC 1831 T was not observed on ISP2 agar medium.…”

“…T (Mao et al, 2007). The presence of lysophosphatidylethanolamine was useful to distinguish strain CPB1-1 T from the closely related species of the genus Streptomyces as mentioned above.…”