Stress responses in citrus peel: Comparative analysis of host responses to Huanglongbing disease and puffing disorder

Martinelli, Federico; Ibáñez, Ana María; Reagan, Russell L.; Davino, Salvatore; Dandekar, Abhaya M.

doi:10.1016/j.scienta.2015.06.037

Cited by 32 publications

(24 citation statements)

References 45 publications

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“…Metabolic pathway analysis of these differential metabolites revealed that the "phenylpropanoid biosynthesis" metabolic pathway had a p-value < 0.05 for enrichment analysis in all comparison groups, indicating that the relative content of phenylpropanoids was significantly different in all comparison groups ( Figure S5, Table S3). Previous studies have shown that Huanglongbing (HLB) can significantly affected some secondary metabolism such as phenylpropanoid, flavonoid, and terpenoid pathways and primary metabolism such as fatty acid, pentose phosphate, and glycerolipid pathways [29]. The hierarchical cluster analysis of the differential metabolites suggested that those in the HBG, XQG, and ZQG groups and the DHG group showed distinct grouping patterns ( Figure S6, Table S4).…”

Section: Differential Metabolites Between the Three Treatment Groups mentioning

confidence: 86%

Metabolomics Analysis of the Peels of Different Colored Citrus Fruits (Citrus reticulata cv. ‘Shatangju’) During the Maturation Period Based on UHPLC-QQQ-MS

Wang

Huang

et al. 2020

Molecules

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Citrus is a globally consumed fruit with great popularity. Mandarin (Citrus reticulata cv. ‘Shatangju’) is a local variety, and its planting area and yield are the greatest regarding fruit tree planting in Guangdong Province, China. However, its resistance to Huanglongbing (HLB) is weak. After infection by HLB, the fruits cannot develop normally. In this study, four kinds of fruits were classified as HBG, XQG, ZQG, and DHG, according to the color of their peels. The metabolomes of the three abnormally colored groups (HBG, XQG, and ZQG) and the normally colored group (DHG) were compared using a UPLC-QQQ-MS-based metabolomics approach. In total, 913 metabolites were identified and classified into 23 different categories, including phenylpropanoids and flavonoids; among them, 215 (HBG, 177; XQG, 124; and ZQG, 62) metabolites showed differential accumulation in the three comparison groups (HBG/XQG/ZQG versus DHG). A total of 2 unique metabolites, O-caffeoyl maltotriose and myricetin were detected only in DHG samples. When comparing HBG with DHG, there were 109 decreased and 68 increased metabolites; comparing XQG with DHG, there were 88 decreased and 36 increased metabolites; comparing ZQG with DHG, 41 metabolites were decreased, and 21 metabolites were increased. Metabolic pathway enrichment analysis of these differential metabolites showed significant enrichment of the “phenylpropanoid biosynthesis” pathway in all comparison groups. The hierarchical cluster analysis of the differential metabolites of the four groups showed a clear grouping patterns. The relative contents of three phenylpropanoids, four flavonoids, two alkaloids, one anthocyanin, and two other metabolites were significantly different between each comparison group. This study might provide fundamental insight for the isolation and identification of functional compounds from the peels of citrus fruit infected with HLB and for in-depth research on the effect of HLB on the formation of fruits pigment and the development of HLB-resistant citrus varieties.

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Section: Differential Metabolites Between the Three Treatment Groups mentioning

confidence: 86%

Metabolomics Analysis of the Peels of Different Colored Citrus Fruits (Citrus reticulata cv. ‘Shatangju’) During the Maturation Period Based on UHPLC-QQQ-MS

Wang

Huang

et al. 2020

Molecules

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“…Transcriptome changes of sucrose and starch metabolism are related to puffing or section-drying disorder, and the gene expression related to starch output and sugar transformation is enhanced in puffing fruit as indicated by gene chip analysis [30]. Puffing mostly alters primary metabolism, such as fatty acid, pentose phosphate, and glyceride [31]. Besides, some other studies have shown that lignin metabolism is closely associated with the process of juice sac granulation [13,14,16,26].…”

Section: Gene Expression In Late-ripening Navel Orange Fruit Under Lomentioning

confidence: 99%

Transcriptome Analysis Unravels Metabolic and Molecular Pathways Related to Fruit Sac Granulation in a Late-Ripening Navel Orange (Citrus sinensis Osbeck)

Wang

et al. 2020

Plants

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Lanelate navel orange (Citrus sinensis Osbeck) is a late-ripening citrus cultivar increasingly planted in China. The physiological disorder juice sac granulation often occurs in the fruit before harvest, but the physiological and molecular mechanisms underlying this disorder remain elusive. In this study, we found that fruit granulation of the late-ripening navel orange in the Three Gorges area is mainly caused by the low winter temperature in high altitude areas. Besides, dynamic changes of water content in the fruit after freezing were clarified. The granulation of fruit juice sacs resulted in increases in cell wall cellulose and decreases in soluble solid content, and the cells gradually became shrivelled and hollow. Meanwhile, the contents of pectin, cellulose, and lignin in juice sac increased with increasing degrees of fruit granulation. The activities of pectin methylesterase (PME) and the antioxidant enzymes peroxidase (POD), superoxide dismutase, and catalase increased, while those of polygalacturonase (PG) and cellulose (CL) decreased. Furthermore, a total of 903 differentially expressed genes were identified in the granulated fruit as compared with non-disordered fruit using RNA-sequencing, most of which were enriched in nine metabolic pathways, and qRT-PCR results suggested that the juice sac granulation is closely related to cell wall metabolism. In addition, the expression of PME involved in pectin decomposition was up-regulated, while that of PG was down-regulated. Phenylalanine ammonia lyase (PAL), cinnamol dehydrogenase (CAD), and POD related to lignin synthesis were up-regulated, while CL involved in cellulose decomposition was down-regulated. The expression patterns of these genes were in line with those observed in low-temperature treatment as revealed by qRT-PCR, further confirming that low winter temperature is associated with the fruit granulation of late-ripening citrus. Accordingly, low temperature would aggravate the granulation by affecting cell wall metabolism of late-ripening citrus fruit.

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“…Expected outcomes of this objective will be (1) the identification of thousands of methylation sites and single nucleotide polymorphisms (SNPs) linked with tolerance/susceptibility to these environmental constraints, (2) the establishment of the link between epigenetic information and previous identified molecular clusters in model legumes such as bean or chickpea (Table 1). Similar approaches have been performed to identify key players in molecular regulatory networks in plant responses to environmental stresses [35][36][37]. Thanks to the agronomic evaluation of first objective, we would select one bean genotype with high resistance and one genotype with high susceptibility to drought stress.…”

Section: Second Objective: Epigenomic Adaptation To Climate Changementioning

confidence: 99%

Proposed Research for Innovative Solutions for Chickpeas and Beans in a Climate Change Scenario: The Mediterranean Basin

et al. 2020

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In order to gain insight into the complex molecular networks driving legume adaptation caused by climate change, it is necessary to deeply characterize the existing germplasm in response to the environmental constraint predicted to worsen in the near future: drought. In this study, we propose to perform a three-year deep agronomic characterization of local genotypes of selected legumes in abiotic stressing conditions through controlled and field experiments conducted in several countries of the Mediterranean basin (Italy, Spain, Algeria, Tunisia, Turkey, Lebanon, and Croatia). These phenotypic analyses will be integrated with a multi-omic approach aiming at identifying the key players involved in the modulation of the analyzed traits that includes the analysis of the plant methylome, transcriptome, and proteome. Following this approach, we propose to deliver epigenomic markers linked with rapid adaptation mechanisms in response to drought. Besides, new genetic variability by breeding could be created in stressing conditions and produce the basis for the obtainment of more productive cultivars in worsening environments. The epigenetic marks identified in "omic" activities will be validated in molecular marker-assisted selection in F2-F4 populations. Finally, specific rhizobia strains for the best evaluated genotypes will be identified in order to enhance symbiotic nitrogen fixation in drought stress conditions with selected cultivars. -M.; visualization, F.M. and F.P.-M. All authors have read and agreed to the published version of the manuscript.Funding: This research received no external funding. Conflicts of Interest:The authors declare no conflict of interest.

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Stress responses in citrus peel: Comparative analysis of host responses to Huanglongbing disease and puffing disorder

Cited by 32 publications

References 45 publications

Metabolomics Analysis of the Peels of Different Colored Citrus Fruits (Citrus reticulata cv. ‘Shatangju’) During the Maturation Period Based on UHPLC-QQQ-MS

Metabolomics Analysis of the Peels of Different Colored Citrus Fruits (Citrus reticulata cv. ‘Shatangju’) During the Maturation Period Based on UHPLC-QQQ-MS

Transcriptome Analysis Unravels Metabolic and Molecular Pathways Related to Fruit Sac Granulation in a Late-Ripening Navel Orange (Citrus sinensis Osbeck)

Proposed Research for Innovative Solutions for Chickpeas and Beans in a Climate Change Scenario: The Mediterranean Basin

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