Stretchable electrical cell-substrate impedance sensor platform for monitoring cell monolayers under strain

Zhou, Chunlin; Bette, Sebastian; Babendreyer, Aaron; Hoffmann, Christina; Gerlach, S.; Kremers, Tom; Ludwig, Andreas; Hoffmann, Bernd; Merkel, Rudolf; Uhlig, Stefan; Schnakenberg, Uwe

doi:10.1016/j.snb.2021.129656

Cited by 6 publications

(5 citation statements)

References 80 publications

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“…For example, Zhou et al developed a stretchable ECIS platform to monitor cell monolayers under strain, with interdigitated gold electrodes integrated onto PDMS membrane. 278 As chips with stretchable PDMS membrane for modeling gut and lung barriers are well-established, 76,91 one could extrapolate an integration of the stretchable ECIS electrodes into models of the intestinal barrier and lung, monitoring the integrity of the stretched monolayer in real time. Flexible electrodes can also be fabricated on substrates that emulate other microenvironmental features like the topography and mechanical properties of the extracellular matrix.…”

Section: Flexible Electrodesmentioning

confidence: 99%

Bridging barriers: advances and challenges in modeling biological barriers and measuring barrier integrity in organ-on-chip systems

Ugodnikov,

Persson,

Simmons

2024

Lab Chip

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Section: Flexible Electrodesmentioning

confidence: 99%

Bridging barriers: advances and challenges in modeling biological barriers and measuring barrier integrity in organ-on-chip systems

Ugodnikov,

Persson,

Simmons

2024

Lab Chip

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“…In a recent study, stretchable electrodes coupled with an ultra-elastic PDMS surface were used to perform electrical impedance sensing of a cell monolayer under stretching conditions. 140 The impedance spectra of Madin-Darby canine kidney (MDCK) cell monolayers were recorded and compared to those of A549 cell monolayers under a single-axle mechanical strain of above 20%. 140 The results showed that the changes in impedance magnitude and phase under strain depended on the type of epithelial cells studied and the tightness of the cell junctions.…”

Section: Adherent Cell Layer Analysismentioning

confidence: 99%

“…140 The impedance spectra of Madin-Darby canine kidney (MDCK) cell monolayers were recorded and compared to those of A549 cell monolayers under a single-axle mechanical strain of above 20%. 140 The results showed that the changes in impedance magnitude and phase under strain depended on the type of epithelial cells studied and the tightness of the cell junctions.…”

Section: Adherent Cell Layer Analysismentioning

confidence: 99%

Concepts, electrode configuration, characterization, and data analytics of electric and electrochemical microfluidic platforms: a review

Nguyen

Thi

et al. 2023

Analyst

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“…[26,27] Two base to cure ratios are used in the work, 25:1 and 10:1, with an elastic modulus of 0.98 and 2.61 MPa, respectively, as measured by Wang et al [27] With these tunable mechanical properties and the bio-compatiability PDMS is perfect material to be used as a stretchable substrate for a cell stretcher. It has been used in many applications in cell biology (see for example [5,[11][12][13]28] )…”

Section: Polydimethylsiloxane (Pdms)mentioning

confidence: 99%

“…To experimentally simulate conditions of constant strain and stress, cell stretcher are used. These typically consist of a stretchable elastomer as a substrate that is either uniaxially [5,[10][11][12][13] or equiaxially stretched. [14,15] As the substrate is stretched, cells attached to it are also stretched.…”

Section: Introductionmentioning

confidence: 99%

Epithelial Cells under Mechanical Strain: an Investigation

Bodenschatz¹

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Cells are the minimal living unit of the body. Each cell plays a vital role that must withstand various stresses, from chemical environments to mechanical strain and temperature variations. One of these vital cell types in the mammalian body is the epithelial cell, which lines the inner and outer surfaces of organs and cavities. These form tightly connected cell monolayers that produce a barrier that allows for directed transport in the body.In this cell resolved study, confluent epithelial cells (MDCK II) were investigated under uniaxial strain. Since little is known about the combined effect of temperature and strain on epithelial cell layers, measurements were performed at 25 • C, 37 • C, and 39 • C under strain. For this purpose, a uniaxial cell stretcher with elastic polydimethylsiloxane membranes was designed, built, characterized, and used for studies at axial strains up to 45 %. The stretcher allowed for both geometric characterization with optical microscopy and mechanical characterization with atomic force microscopy of single cells in a confluent layer, before, during the 30 minute strain application, and directly after strain release.With strain application, the cell geometry changed with an increase in cell area and shift of the cell orientation in the direction of strain. These changes were temperature and strain-dependent, with smaller geometric changes at 25 • C and low strain. After strain release, the cells returned largely to their before strain values. Atomic force microscopy indentation experiments at 37 • C showed that during strain, the pre-stress T 0 and area compressibility modulus K A0 increased while the cell fluidity β remained constant. After strain release all parameters except tension returned to the before strain values. This increase in stiffness at constant fluidity can also be observed in the treatment of cells with methyl-beta-cyclodextrin, which reduced the excess membrane area of the cells.Therefore the changes in mechanical properties can be largely attributed to a reduction in excess membrane area. When cooling the cells to 25 • C at low strains, a shift in the scaling of stiffness and fluidity can be observed, which disappears at high strains. This change might be attributed to a lipid membrane phase transition at 27 • C which restricts the recruitment of excess membrane area at low strains. When the stain is increased, a shear-induced phase transition might occur, resulting in similar properties as at 37 • C.Overall, these results point to a passive reaction of the cells to external stress, namely recruitment of the excess surface area in response to strain.

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Stretchable electrical cell-substrate impedance sensor platform for monitoring cell monolayers under strain

Cited by 6 publications

References 80 publications

Bridging barriers: advances and challenges in modeling biological barriers and measuring barrier integrity in organ-on-chip systems

Bridging barriers: advances and challenges in modeling biological barriers and measuring barrier integrity in organ-on-chip systems

Concepts, electrode configuration, characterization, and data analytics of electric and electrochemical microfluidic platforms: a review

Epithelial Cells under Mechanical Strain: an Investigation

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