Strontium functionalized scaffold for bone tissue engineering

Prabha, Rahul D; Nair, Bindu P.; Ditzel, Nicholas; Kjems, Jørgen; Nair, Prabha D.; Kassem, Moustapha

doi:10.1016/j.msec.2018.09.054

Cited by 29 publications

(21 citation statements)

References 47 publications

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“…It is of low cost, has a longer shelf life, and presents low systemic risk compared to growth factors. These added benefits make Sr as therapeutic agent attractive in tissue engineering and regenerative medicine applications (Jiménez et al, 2019;Prabha et al, 2019;Mao et al, 2020). However, the mechanism of osteoinduction of SrCaPs remains complicated because inflammation and angiogenesis accompany the entire process of bone healing.…”

Section: Resultsmentioning

confidence: 99%

Building Osteogenic Microenvironments With Strontium-Substituted Calcium Phosphate Ceramics

Wan

Wang

Sun

et al. 2020

Front. Bioeng. Biotechnol.

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Bioceramics have experienced great development over the past 50 years. Modern bioceramics are designed to integrate bioactive ions within ceramic granules to trigger living tissue regeneration. Preclinical and clinical studies have shown that strontium is a safe and effective divalent metal ion for preventing osteoporosis, which has led to its incorporation in calcium phosphate-based ceramics. The local release of strontium ions during degradation results in moderate concentrations that trigger osteogenesis with few systemic side effects. Moreover, strontium has been proven to generate a favorable immune environment and promote early angiogenesis at the implantation site. Herein, the important aspects of strontium-enriched calcium phosphate bioceramics (Sr-CaPs), and how Sr-CaPs affect the osteogenic microenvironment, are described.

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Section: Resultsmentioning

confidence: 99%

Building Osteogenic Microenvironments With Strontium-Substituted Calcium Phosphate Ceramics

Wan

Wang

Sun

et al. 2020

Front. Bioeng. Biotechnol.

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“…This finding was further affirmed by the fact that CLN increment in the scaffolds, such as PC‐20 with highest CLN amount, generated a better synthetic environment for hFOB to attach and grow (Figure a). In a similar study, Prabha et al () fabricated a laponite (LPN) clay containing PCL scaffolds having high porosity via solvent casting and lyophilization (Prabha et al, ). At the end of 7 days of incubation, they observed that cells increased in number at around 1.5 fold of initial seeded number.…”

Section: Resultsmentioning

confidence: 99%

Composite clinoptilolite/PCL‐PEG‐PCL scaffolds for bone regeneration: In vitro and in vivo evaluation

Pazarçeviren

Dikmen

Altunbaş

et al. 2019

J Tissue Eng Regen Med

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In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time.Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compression molding technique with varying CLN contents. We hypothesized that CLN reinforcement in a composite scaffold will improve bone regeneration and promote repair. Therefore, the scaffolds were analyzed for compressive strength, biodegradation, biocompatibility, and induction of osteogenic differentiation in vitro. CLN inclusion in PC-10 (10% w/w) and PC-20 (20% w/w) scaffolds revealed 54.7% and 53.4% porosity, higher dry (0.62 and 0.76 MPa), and wet (0.37 and 0.45 MPa) compressive strength, greater cellular adhesion, alkaline phosphatase activity (2.20 and 2.82 mg/g DNA /min), and intracellular calcium concentration (122.44 and 243.24 g Ca/mg DNA ). The scaffolds were evaluated in a unicortical bone defect at anterior aspect of proximal tibia of adult rabbits 4 and 8 weeks postimplantation. Similar to in vitro results, CLN-containing scaffolds led to efficient regeneration of bone in a dose-dependent manner. PC-20 demonstrated highest quality of bone union, cortex development,and bone-scaffold interaction at the defect site. Therefore, higher CLN content in PC-20 permitted robust remodeling whereas pure PCEC (PC-0) scaffolds displayed fibrous tissue formation. Consequently, CLN was proven to be a potent reinforcement in terms of promoting mechanical, physical, and biological properties of polymer-based scaffolds in a more economical, easy-to-handle, and reproducible approach.

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“…Therefore, our initial results indicated that the ions released from SrCS scaffolds had a role to play in enhancing cellular behaviors such as cell proliferation, differentiation and even osteogenesis. Different levels of Sr were reported to having various effects and it was shown that sufficient levels of Sr ions were able to allow osteogenesis but too high levels of Sr would lead to defective bone mineralization [38].…”

Section: Discussionmentioning

confidence: 99%

The Calcium Channel Affect Osteogenic Differentiation of Mesenchymal Stem Cells on Strontium-Substituted Calcium Silicate/Poly-ε-Caprolactone Scaffold

Tp¹,

Huang

Kao

et al. 2020

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There had been a paradigm shift in tissue engineering studies over the past decades. Of which, part of the hype in such studies was based on exploring for novel biomaterials to enhance regeneration. Strontium ions have been reported by others to have a unique effect on osteogenesis. Both in vitro and in vivo studies had demonstrated that strontium ions were able to promote osteoblast growth, and yet at the same time, inhibit the formation of osteoclasts. Strontium is thus considered an important biomaterial in the field of bone tissue engineering. In this study, we developed a Strontium-calcium silicate scaffold using 3D printing technology and evaluated for its cellular proliferation capabilities by assessing for protein quantification and mineralization of Wharton’s Jelly mesenchymal stem cells. In addition, verapamil (an L-type of calcium channel blocker, CCB) was used to determine the mechanism of action of strontium ions. The results found that the relative cell proliferation rate on the scaffold was increased between 20% to 60% within 7 days of culture, while the CCB group only had up to approximately 10% proliferation as compared with the control specimen. Besides, the CCB group had downregulation and down expressions of all downstream cell signaling proteins (ERK and P38) and osteogenic-related protein (Col I, OPN, and OC). Furthermore, CCB was found to have 3–4 times lesser calcium deposition and quantification after 7 and 14 days of culture. These results effectively show that the 3D printed strontium-contained scaffold could effectively stimulate stem cells to undergo bone differentiation via activation of L-type calcium channels. Such results showed that strontium-calcium silicate scaffolds have high development potential for bone tissue engineering.

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Strontium functionalized scaffold for bone tissue engineering

Cited by 29 publications

References 47 publications

Building Osteogenic Microenvironments With Strontium-Substituted Calcium Phosphate Ceramics

Building Osteogenic Microenvironments With Strontium-Substituted Calcium Phosphate Ceramics

Composite clinoptilolite/PCL‐PEG‐PCL scaffolds for bone regeneration: In vitro and in vivo evaluation

The Calcium Channel Affect Osteogenic Differentiation of Mesenchymal Stem Cells on Strontium-Substituted Calcium Silicate/Poly-ε-Caprolactone Scaffold

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