Structural analysis of molecular species of nocardomycolic acids from<i>Nocardia erythropolis</i> by the combined system of gas chromatography and mass spectrometry

Yano, Ikuya; Saito, Kunihiko; Furukawa, Yoshiko; Kusunose, Masamichi

doi:10.1016/0014-5793(72)80140-6

Cited by 36 publications

(25 citation statements)

References 11 publications

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“…In conclusion, from these results and the previous reports [ 10,12], a highly complex mixture of mycolic acid-type fatty acids ranging from C20 to Cso can be fully separated according to their total carbon numbers, and the branched-chain structures of individual molecular species can be also determined rapidly using the direct combination system of gas chromatography and mass spectrometry.…”

Section: B)supporting

confidence: 73%

“…Conditions for gas-liquid chromatography are described in the text. Furthermore, from the total carbon numbers of original fatty acid corresponding to peak 2, the ion peaks derived from/3 -7 cleavage are also expected [10]. This peak can be observed at m/e 343 in fig.…”

Section: Resultsmentioning

confidence: 80%

“…However, our results indicated that those of corynomycolic acids from C. ulcerans covered a very wide range, and even the acid of same carbon numbers recognized as a single peak on the gas chromatogram were shown to be a mixture of several isomers with different branch structures, as in the case of nocardomycolic acids [10] ;…”

Section: B)mentioning

confidence: 69%

“…The polar esters were recovered from the thin-layer plate with diethyl ether. After the solution was evaporated to dryness, the residues were trimethylsilylated by the method described previously [ 10].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we have succeeded in separating 15 molecular species of nocardomycolic acids and clarified the branched-chain structures of each component by the combined system of gas chromatography and mass spectrometry [ 10]. The present paper describes that the homologues of corynomycolic acids obtained from Corynebacterium ulcerans can be also separated clearly into 12 major acids according to their total carbon numbers and the degree of unsaturation.…”

Section: Introductionmentioning

confidence: 92%

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Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans

Yano

Saito

1972

FEBS Letters

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Section: B)supporting

confidence: 73%

Section: Resultsmentioning

confidence: 80%

Section: B)mentioning

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

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Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans

Yano

Saito

1972

FEBS Letters

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Separation and analysis of molecular species of mycolic acids inNocardia and related taxa by gas chromatography mass spectrometry

Yano

Kageyama

Ohno

et al. 1978

Biol. Mass Spectrom.

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The gas chromatographic separation and mass spectrometric identification of mycolic acids ranging from Czs to CaS in Nocardia and related acid-fast groups of bacteria was established. Using this method, the molecular species of mycolic acids were clearly separated as the trimethylsilyl (TMS) derivatives of the methyl esters, according to their total carbon numbers. From the [Mlt, [M-15]+ and [M-90]t ions produced by gas chromatography mass spectrometry, the total carbon and double bond numbers were determined, while the straight and branched chain structures were identified by the mass fragment ions due to C2-C3 cleavageBased on the molecular species composition, the average carbon numbers of the mycolic acids from 16 strains of Nocardia and related taxa (Mycobacterium rhodochrous complex and Gordona bronchialis) were determined. Each species of bacteria was demonstrated to possess a characteristic profile of mycolic acid composition, and they were shown to be classified approximately into at least four groups: (1) CZs to C48 (average carbon numbers C3,-,,) mostly saturated for Nocardia erythropolis and several 'Mycobacterium rhodochrous complex'; ( 2 ) CM to Cs0 (average C43-45) monoenoic and dienoic for N. rubra, N. corallina and N. lutea; (3) C44 to CS8 (average C5,-5z) monoenoic to tetraenoic for N. asteroides and 'true' nocardiae; (4) c 5 6 to Css (average c62-a) monoenoic to pentaenoic for N. polychromogenes and Gordona bronchialis. The results indicate that the structural determination of mycolic acids by gas chromatography mass spectrometry can be one of the most useful criteria for the chemotaxonomy of Nocardia and related bacteria.

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Analysis of Bacterial Metabolites and Components by Computerized GC/MS System — From Shorter Chain Acids to Very Long-Chain Compounds up to C80

Yano

1985

Rapid Methods and Automation in Microbiology and Immunology

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Structural analysis of molecular species of nocardomycolic acids fromNocardia erythropolis by the combined system of gas chromatography and mass spectrometry

Cited by 36 publications

References 11 publications

Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans

Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans

Separation and analysis of molecular species of mycolic acids inNocardia and related taxa by gas chromatography mass spectrometry

Analysis of Bacterial Metabolites and Components by Computerized GC/MS System — From Shorter Chain Acids to Very Long-Chain Compounds up to C80

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