Structural changes in the rat testis caused by diazinon and selenium

Abstract

<div style="mso-element: frame; mso-element-frame-width: 498.1pt; mso-element-frame-height: 318.1pt; mso-element-frame-vspace: 3.1pt; mso-element-wrap: around; mso-element-anchor-vertical: page; mso-element-anchor-horizontal: page; mso-element-left: 52.45pt; mso-element-top: 55.2pt; mso-height-rule: exactly;"><span style="font-size: medium;"><p class="MsoNormal">The aim of this study was to find the structural changes in the rat testis after a diazinon, selenium and their combined administration. The testis structural changes after the diazinon intraperitoneal administration of 20 mg/kg b.w., selenium 2 mg/kg b.w. and both diazinon 20 mg/kg b.w. and selenium 2 mg/kg b.w. were evaluated by histological and morphometric methods in light microscopy. 36 hours after the diazion i.p. administration, the vacuolization of the seminiferous epithelium, evacuation of germ cells into the tubule lumen, epithelium necrosis and disintegration, interstitium extension and fibrotization were observed. The germ cells released from the basal lamina and subsequently they were visible in the tubule lumen. Blood vessels were damaged and morphometric analysis have shown their significant dilatation (P<0.05). Diazinon causes the damage of the germinal epithelium in the testes leading to the spermatogenesis failure. The infertility can then appear. In selenium treated group disintegration of cellular associations in the seminiferous epithelium, damaged and separating spermatids lines, reduced spermatogenesis and significant vacuolization of seminiferous epithelium (P<0.0001) were observed. Similar changes as in selenium threated group occured, significant vacuolization of seminiferous epithelium (P<0.05) was seen. In combined diazinon and selenium treated group protective effects of selenium in seminiferous epithelium and interstitium were noticed. Further investigation of diazinon and selenium is needed to practical use this results.</p><p class="MsoNormal">&nbsp;</p><p class="MsoNormal"><span style="font-size: 12px;"><strong>doi:10.5219/44</strong></span></p> <p class="MsoNormal">&nbsp;</p></span></div

Simultaneous subchronic exposure to selenium and diazinon as possible risk factor for osteoporosis in adult male rats

“…Results obtained by Cabaj et al . [ 26 ] demonstrate that the blood vessels of testes in rats co-administered by Se and DZN (at the same levels as were used in our study) were damaged and significantly dilated. Additionally, Ruseva et al .…”

Toxic effects of some pesticides on the enzymatic activities and spermatogenesis of the land snail Monacha cantiana

“…In this study, LC25 of Diazinon was tested to reveal its histopathological effect on the ovotestis of M. cantiana because of its direct effect on reproductive organs. In rats, intraperitoneal administration of Diazinon damaged their testis germinal epithelium leading to spermatogenesis failure after 36h and resulted in infertility (Cabaja et al, 2010). Severe damages occurred in the hermaphrodite gland of B. alexandrina treated with both Albendazode and Nitazode pesticides (El-Enain et al, 2015).…”

Alterations in the fatty acid profile, antioxidant enzymes and protein pattern of Biomphalaria alexandrina snails exposed to the pesticides diazinon and profenofos

“…Diazinon was highly toxic to the snakehead fish (Channa striata) in the Mekong River Delta, Vietnam, with a 96-h median lethal concentration (LC 50 ) of 0.79 mg/L, and caused long-term ChE inhibition (Alahyary et al, 2008). In rats, diazinon intraperitoneal administration damaged their testis germinal epithelium leading to spermatogenesis failure after 36 h, then infertility can appear (Cabaj et al, 2010). Profenofos is moderately toxic to birds and has low mammalian toxicity with good insecticidal properties (Tomlin, 1997).…”