Structural characterisation and immunomodulatory activity of a neutral polysaccharide from Sambucus adnata Wall

Yuan, Lei; Zhong, Zheng-Chang; Liu, Yu

doi:10.1016/j.ijbiomac.2019.11.021

Cited by 32 publications

(16 citation statements)

References 37 publications

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“…→4)- β -Gal p -(1→ (D), →3,6)- β -Gal p -(1→ (E), and →3)- β -Gal p -(1→ (F) were matched to the signals of δ H /δ C 4.56/105.74, 4.52/104.69 and 4.4/104.48, respectively. Similar signals of anomeric proton and carbon have been reported in some literature [ 26 , 27 ]. Residue G was found to have a downfield H-1 (4.45 ppm) and a downfield C-1 (103.63 ppm), consistent with →4)- β -Glc p -(1→ [ 28 ].…”

Section: Resultssupporting

confidence: 88%

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The extraction and characterization of new bioactive plant-derived polysaccharides with the potential for use as functional foods and medicine have attracted much attention. In the present study, A novel acidic polysaccharide (RPP-3a) with a weight-average molecular weight (Mw) of 88,997 Da was isolated from the raspberry pulp. RPP-3a was composed of rhamnose, arabinose, galactose, glucose, mannose, and galacturonic acid at a molar ratio of 13.1:28.6:16.8:1.4:6.2:33.9. Structural analysis suggested that the RPP-3a backbone was composed of repeating units of →4)-β-Galp-(1→3,4)-α-Rhap-(1→[4)-α-GalAp-(1→4)-α-GalAp-(1→]n with branches at the C-4 position of rhamnose. The side chain of RPP-3a, containing two branch levels, was comprised of α-Araf-(1→, →5)-α-Araf-(1→, →3,5)-α-Araf-(1→, →3)-β-Galp-(1→, →3,6)-β-Galp-(1→, →4)-β-Glcp-(1→, and →2,6)-α-Manp-1→ residues. RPP-3a exhibited moderate reducing power and strong hydroxyl and superoxide anion radical scavenging abilities. RPP-3a significantly promoted the viability of RAW264.7 macrophages by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) at both the expression and transcriptional levels. In summary, the immunostimulatory and antioxidant activities make RPP-3a a viable candidate as a health-beneficial functional dietary supplement.

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Section: Resultssupporting

confidence: 88%

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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“…Previously, many studies have reported that neutral polysaccharides have potential as an immunomodulator or supplement in functional food to enhance immunity. For example, a neutral polysaccharide SMP-0b extracted from Solanum muricatum could significantly stimulate proliferation and NO production of RAW 264.7 macrophage cells (Yue et al, 2020); a neutral polysaccharide SPW-2, purified from the leaves of Sambucus adnata Wall, exerted an immunomodulatory effect by activating macrophages and enhancing the host immune system function (Yuan et al, 2020); a neutral polysaccharide WSRP-1b from Kushui rose waste possessed immunomodulatory activity by enhancing phagocytosis of macrophages, increasing the production of ROS, NO, cytokines (IL-6 and TNF-α), and activating the NF-κB signaling pathway (Wu et al, 2020). Inspired by these studies of the immunomodulatory activity of neutral polysaccharides, we also examined the immunomodulatory ability of LHPW through a series of experiments.…”

Section: Discussionmentioning

confidence: 99%

Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide From Lycopi Herba

Zhang

et al. 2021

Front. Pharmacol.

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Lycopi Herba has been broadly used as a traditional medicinal herb in Asia due to its ability to strengthen immunity. However, it is still obscure for its material basis and underlying mechanisms. Polysaccharide, as one of the most important components of most natural herbs, usually contributes to the immunomodulatory ability of herbs. Here, we aimed to detect polysaccharides from Lycopi Herba and examine their potential immunomodulatory activity. A novel polysaccharide (LHPW) was extracted from Lycopi Herba and purified by DEAE-52 cellulose chromatography and G-100 sephadex. According to physicochemical methods and monosaccharide composition analysis, LHPW was mainly composed of galactose, glucose, fructose, and arabinose. NMR and methylation analyses indicated that LHPW was a neutral polysaccharide with a backbone containing →3,6)-β-D-Galp-(1→, →4)-β-D-Galp-(1→ and →4)-α-D-Glcp-(1→, with the branches of →1)-β-D-Fruf-(2→ and →6)-α-D-Galp-(1→. Immunological tests indicated that LHPW could activate macrophage RAW264.7 and promote splenocyte proliferation. This study discovered a novel polysaccharide from Lycopi Herba and showed it was a potential immunomodulator.

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“…Accumulating evidence suggests that plant-derived polysaccharides, with relatively low toxicity and side effects, possess potent immunomodulatory activity by enhancing or activating the immune responses of macrophages [ 8 , 11 ]. For instance, a polysaccharide from the wall of Sambucus adnate exerts an immunomodulatory effect by activating macrophages and enhancing the host’s immune system function [ 12 ]. Similarly, the polysaccharides obtained from Radix aconiti significantly promote macrophage phagocytosis and increase the secretion of biological factors [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The discovery of safe and effective plant polysaccharides with immunomodulatory effects has become a research hotspot. Raspberry is an essential commercial fruit and is widely distributed, cultivated, and consumed worldwide. In the present study, a homogeneous acidic polysaccharide (RPP-2a), with a weight-average molecular weight (Mw) of 55582 Da, was isolated from the pulp of raspberries through DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. RPP-2a consisted of rhamnose, arabinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid, with a molar ratio of 15.4:9.6:7.6:3.2:9.1:54.3:0.8. The results of Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometer (GC-MS), 1D-, and 2D-nuclear magnetic resonance (NMR) analyses suggested that the backbone of RPP-2a was primarily composed of →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, →4)-α-D-GalAp-(1→, and →3,4)-α-D-Glcp-(1→ sugar moieties, with side chains of α-L-Araf-(1→, α-L-Arap-(1→, and β-D-Galp-(1→3)-β-D-Galp-(1→ residues linked to the O-4 band of rhamnose and O-3 band of glucose residues. Furthermore, RPP-2a exhibited significant macrophage activation activity by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the expression of inducible nitric oxide synthase (iNOS) and cytokines at the transcriptional level in RAW264.7 cells. Overall, the results indicate that RPP-2a can be utilized as a potential natural immune-enhancing agent.

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Structural characterisation and immunomodulatory activity of a neutral polysaccharide from Sambucus adnata Wall

Cited by 32 publications

References 37 publications

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide From Lycopi Herba

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

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