Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara

Chouana, Toufik; Pierre, Guillaume; Vial, Christophe; Gardarin, Christine; Wadouachi, Anne; Cailleu, Dominique; Cerf, Didier Le; Boual, Zakaria; Hadj, Mohamed Didi Ould El; Michaud, Philippe; Delattre, Cédric

doi:10.1016/j.carbpol.2017.08.003

Cited by 35 publications

(14 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in 13 C NMR spectrum (Figure 3b), the anomeric signal at 99.51 ppm and 100. Compared to other galactomannans extracted from the seeds of different leguminous plants, M/G ratio of WSPAM1 was slightly elevate to those estimated for Astragalus gombo Bunge, Sesbania virgata and Leucaena leucocephala to 1.7, 1.5 and 1.6, respectively [50][51][52][53]. The purity of galactomannan and the M/G ratios of seeds from Fabaceae was related to plant origin and extraction processes [40,53,54].…”

Section: H 13 C Nmr and Hsqc Analysismentioning

confidence: 99%

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Chakou

Boual

Hadj

et al. 2021

Plants

View full text Add to dashboard Cite

The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Alhagi maurorum Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were characterized using Fourier transform infrared, 1H/13C Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering. The capacity of those fractions to inhibit α-amylase activity and thermally induced Bovine Serum Albumin denaturation were also investigated. WSPAM1 and WSPAM2 were galactomannans with a mannose/galactose ratio of 2.2 and 2.4, respectively. The SEC-MALLS analysis revealed that WSPAM1 had a molecular weight of 1.4 × 106 Da. The investigations highlighted antinflammatory and antihyperglycemic effects in a dose-dependant manner of WSPAM1 and WSPAM2.

show abstract

Section: H 13 C Nmr and Hsqc Analysismentioning

confidence: 99%

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Chakou

Boual

Hadj

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…The wide range of efficiency may be probably due to the structural heterogeneity as stated above [63]. Note that immunomodulatory polysaccharides do not cause damage and additional stress to the body, because they only act as modifiers of the biological response [24].…”

Section: Screening the Biological Potential Of Polysaccharides: Randomly Or Not?mentioning

confidence: 99%

“…Thus, numerous reports on the biological activity of polysaccharides extracted from plants and used in traditional medicines have been published. Minor structural differences, e.g., monosaccharide composition and distribution, chain conformation, macromolecular behavior, branching degree and pattern, chemical structures, and presence of non-carbohydrate substituents [13,24], can significantly affect the biological activity of polysaccharides [64]: this is the so-called structure-function relationship. Changes of sulfate content, molecular weight and/or molar ratio greatly affect both biological functions and rheological behavior.…”

Section: Screening the Biological Potential Of Polysaccharides: Randomly Or Not?mentioning

confidence: 99%

“…WSPF was made up of a linear backbone of β-(1,4)-D-Manp with α-(1,6)-D-Galp side chains. Besides, Chouana et al extracted a water-soluble polysaccharide (WSP) from A. gombo seeds [24]. The fraction had an average molecular weight of 1.1 × 10 6 g/mol and was composed of Gal (37% ± 0.9) and Man (63% ± 0.7), with a Man:Gal ratio close to 1.7:1.…”

Section: Astragalusmentioning

confidence: 99%

See 1 more Smart Citation

Polysaccharides and Derivatives from Africa to Address and Advance Sustainable Development and Economic Growth in the Next Decade

Sarraf¹,

Verton²,

Addoun

et al. 2021

Applied Sciences

View full text Add to dashboard Cite

Polysaccharides are highly variable and complex biomolecules whose inventory of structures is still very incomplete, as nature still preserves unexplored biotopes. Plants, macroalgae and microalgae are an integral part of the daily life of human being regardless of culture, time, or knowledge development of a country. Natural medicine is an ancestral knowledge widely distributed throughout the world, handed down for centuries from generation to generation by those commonly referred to as “nganga” healers or shamans. It is also called alternative medicine or traditional medicine, and has been associated for millennia to legends. This review gives an emphasis regarding the ethnobotanic approach associated to the structural variability of poly- and oligosaccharides for designing the new polysaccharide-based drugs and hydrocolloids of tomorrow. The guiding thread is to survey the potential of plants (and some macroalgae) from Africa as a source of polysaccharides with original structures and, secondly, to correlate these structures with biological and/or functional properties in particular to address and advance the sustainable development and economic growth of mankind.

show abstract

“…The critical overlap concentration C* (g/L or %) was evaluated by plotting the specific viscosity (η sp , (Pa.s)) vs. JSP concentrations from 0.25% to 2.0% (w/v) in water (Figure 9). Figure 9 shows that the specific viscosity increased with increasing polysaccharide concentrations (from 1% to 2.0%, w/v) and the change in slope between the two linear segments helped estimate C* [33,34,59,60].…”

Section: Rheological Behavior Of Jspmentioning

confidence: 99%

Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

et al. 2020

View full text Add to dashboard Cite

A novel sulfated xylogalactan-rich fraction (JSP for J. adhaerens Sulfated Polysaccharide) was extracted from the red Tunisian seaweed Jania adhaerens. JSP was purified using an alcoholic precipitation process and characterized by Attenuated Total Reflectance-Fourier-transform infrared spectroscopy (ATR-FTIR), high-pressure size exclusion chromatography (HPSEC) with a multi-angle laser light scattering (MALLS), gas chromatography coupled to mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR, 1D and 2D). JSP was then evaluated regarding its physicochemical and rheological properties. Results showed that JSP was mainly composed of an agar-like xylogalactan sharing the general characteristics of corallinans. The structure of JSP was mainly composed of agaran disaccharidic repeating units (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→) n and (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→) n , mainly substituted on O-6 of (1,3)-β-d-Galp residues by β-xylosyl side chains, and less with sulfate or methoxy groups. (1,4)-α-l-Galp residues were also substituted by methoxy and/or sulfate groups in the O-2 and O-3 positions. Mass-average and number-average molecular masses (M w ) and (M n ), intrinsic viscosity ([η]) and hydrodynamic radius (R h ) for JSP were, respectively, 8.0 × 10 5 g/mol, 1.0 × 10 5 g/mol, 76 mL/g and 16.8 nm, showing a flexible random coil conformation in solution. The critical overlap concentration C* of JSP was evaluated at 7.5 g/L using the Williamson model. In the semi-diluted regime, JSP solutions displayed a shear-thinning behavior with a great viscoelasticity character influenced by temperature and monovalent salts. The flow characteristics of JSP were described by the Ostwald model. always belongs to the d-series, while the former (α-Gal) can occur as d-(carrageenans) or l-(agarans) series [4][5][6][7]. The main backbone can be substituted by pyruvic acid ketals, sulfate, O-glycosyl and/or methyl groups and also by side chains, giving rise to great structural changes along the galactan chain. These structural changes affect the physicochemical and rheological behaviors of these polymers. So far, it has been reported that sulfated galactans exhibit numerous biological properties, including antioxidant, anticoagulant, antinociceptive, immunomodulatory, anticancer, gastroprotective, anti-inflammatory, antithrombotic and antiproliferative [1,[6][7][8][9][10][11][12][13][14]. Marine seaweed (Phaeophyceae and Rhodophyceae) cell wall polysaccharides also hold great promise as a raw material for feed, food, and biofuel production. Through metabolic engineering, seaweeds (micro-and macroalgae) are attracting growing attention as an alternative source of biofuel by converting the seaweed biomass and, in particular, the cell wall, which is rich in carbohydrates, into liquid biofuels [15].Galactans are currently used as thickening, gelling, and stabilizing agents in food, cosmetic, and pharmaceutical fields [1,10-16]. However, sulfated galactans produced by Corallinales are less studied, probably because ...

show abstract

Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara

Cited by 35 publications

References 51 publications

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds

Polysaccharides and Derivatives from Africa to Address and Advance Sustainable Development and Economic Growth in the Next Decade

Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

Contact Info

Product

Resources

About