Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process

Volpi, Nicola; Galeotti, Fabio; Maccari, Francesca; Capitani, Federica; Mantovani, Veronica

doi:10.1016/j.jpba.2020.113826

Cited by 9 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The source of CS was found in both vertebrates and invertebrates, but their sulfation patterns are varying with the animal species ( Vessella et al, 2019 ). Some terrestrial animal-derived CS preparations are almost only sulfated at C4/C6 of GalNAc residues, for example CS extracted from bovine and porcine trachea ( Volpi et al, 2021 ). In contract, marine animals process a great deal of GAGs with some unusual sulfation patterns ( Valcarcel et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Lin

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Chondroitin sulfate (CS)/dermatan sulfate (DS) is a kind of sulfated polyanionic, linear polysaccharide belonging to glycosaminoglycan. CS/DS sulfatases, which specifically hydrolyze sulfate groups from CS/DS oligo-/polysaccharides, are potential tools for structural and functional studies of CD/DS. However, only a few sulfatases have been reported and characterized in detail to date. In this study, two CS/DS sulfatases, PB_3262 and PB_3285, were identified from the marine bacterium Photobacterium sp. QA16 and their action patterns were studied in detail. PB_3262 was characterized as a novel 4-O-endosulfatase that can effectively and specifically hydrolyze the 4-O-sulfate group of disaccharide GlcUAβ1–3GalNAc(4-O-sulfate) but not GlcUAβ1–3GalNAc(4,6-O-sulfate) and IdoUAα1–3GalNAc(4-O-sulfate) in CS/DS oligo-/polysaccharides, which is very different from the identified 4-O-endosulfatases in the substrate profile. In contrast, PB_3285 specifically hydrolyzes the 6-O-sulfate groups of GalNAc(6-O-sulfate) residues located at the reducing ends of the CS chains and is the first recombinantly expressed 6-O-exosulfatase to effectively act on CS oligosaccharides.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Lin

Zhang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Sulfation patterns also change in most cancerous cells relative to healthy cells [13], as do the cells' response to the presence of CS [14]. Among common terrestrial sources of commercial CS-cows, pigs, and chickens-the degree of sulfation is quite similar, but the chain lengths and 4S:6S ratios differ noticeably between species [15]. CS from sharks and skates tends to have a longer chain length, higher charge density, and more 2S sulfation than terrestrial sources [14].…”

Section: Structure and Characteristics Of Chondroitin And Chondroitin...mentioning

confidence: 99%

Chondroitin Sulfate and Its Derivatives: A Review of Microbial and Other Production Methods

et al. 2022

View full text Add to dashboard Cite

Chondroitin sulfate (CS) is widely used across the world as a nutraceutical and pharmaceutical. Its high demand and potential limitations in current methods of extraction call for an alternative method of production. This review highlights glycosaminoglycan’s structure, its medical significance, animal extraction source, and the disadvantages of the extraction process. We cover alternative production strategies for CS and its precursor, chondroitin. We highlight chemical synthesis, chemoenzymatic synthesis, and extensively discuss how strains have been successfully metabolically engineered to synthesize chondroitin and chondroitin sulfate. We present microbial engineering as the best option for modern chondroitin and CS production. We also explore the biosynthetic pathway for chondroitin production in multiple microbes such as Escherichia coli, Bacillus subtilis, and Corynebacterium glutamicum. Lastly, we outline how the manipulation of pathway genes has led to the biosynthesis of chondroitin derivatives.

show abstract

“…CS is isolated from animal cartilage and purified before being commercially available. 28 The raw material for commercial CS production includes bovine, chicken, porcine, 29 or cartilaginous fishes such as sharks and skate. 30,31 Generally, the steps involved in the isolation of CS include -(a) chemically hydrolyzing the cartilage; (b) breaking down the core made of proteoglycan; (c) eliminating the proteins, followed by recovery of CS; (d) purifying the obtained CS.…”

Section: Extraction Of Csmentioning

confidence: 99%

Chondroitin sulfate-based composites: a tour d’horizon of their biomedical applications

et al. 2022

View full text Add to dashboard Cite

show abstract

Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process

Cited by 9 publications

References 30 publications

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

Chondroitin Sulfate and Its Derivatives: A Review of Microbial and Other Production Methods

Chondroitin sulfate-based composites: a tour d’horizon of their biomedical applications

Contact Info

Product

Resources

About