Structural features, selenization modification, antioxidant and anti-tumor effects of polysaccharides from alfalfa roots

Gao, Pin‐Yi; Bian, Jıanpeng; Xu, Shuping; Liu, Changfeng; Yu-qiu, Sun; Zhang, Gongling; Li, Danqi; Liu, Xuegui

doi:10.1016/j.ijbiomac.2020.01.239

Cited by 59 publications

(46 citation statements)

References 42 publications

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“…ABTS radical scavenging capacity of each DP fraction was carried out with P. Gao et al. 's (2020) method with minor modifications. Potassium persulfate solution (2.6 mmol/L) and ABTS solution (7 mmol/L) were first mixed and kept in the dark at room temperature for 16 hr to generate ABTS radical cations.…”

Section: Methodsmentioning

confidence: 99%

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

Meng

Chen

et al. 2021

Journal of Food Science

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The present study discussed the optimization of the ultrasonic‐assisted extraction of polysaccharides from daylily polysaccharides (DPs). The extracted crude polysaccharides were further separated and purified, and the antioxidant activities including 1,1‐diphenyl‐2‐111 picrylhydrazyl (DPPH) radical scavenging, 2,2'‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) (ABTS) radical scavenging, hydroxyl radical scavenging, and ferric‐reducing antioxidant power (FRAP) activities of the obtained fractions were also evaluated. The results showed that the optimal ultrasonic‐assisted extraction parameters with DPs yield of 15.25 ± 1.13% were water to powder ratio of 25 ml/g, extraction power of 694 W, extraction temperature of 71°C, extraction time of 38 min, and three times extraction. By DEAE Sepharose Fast Flow column, four water‐soluble polysaccharide fractions (DP‐1, DP‐2, DP‐3, and DP‐4) were successfully obtained. Monosaccharide component analysis showed that the four obtained fractions were all hetero‐polysaccharides that mainly contained rhamnose, arabinose, fructose, galactose, glucose, galacturonic acid, and glucuronic acid in different molar ratios. All the four DP fractions did show obvious antioxidant activities in vitro, and the DP‐3 component had relatively high ABTS free radical scavenging activity. Overall, our research showed that DPs could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics. Practical Application This article deals with the optimization of the ultrasonic‐assisted extraction of polysaccharides from daylily and its antioxidant activities. The results showed that the optimal ultrasonic‐assisted extraction yield of DPs was 15.25 ± 1.13%. By DEAE Sepharose Fast Flow column, four water‐soluble polysaccharide fractions were successfully obtained, and all the four DP fractions did show obvious antioxidant activities in vitro. Daylily polysaccharides could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics.

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Section: Methodsmentioning

confidence: 99%

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

Meng

Chen

et al. 2021

Journal of Food Science

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“…Micromorphology of synthetic Se-polysaccharides based upon Medicago sativa roots polysaccharides by NA-SS system was observed using SEM. And they existed in a loose flaky structure, which was different from non-Se-polysaccharides with dense stripped structure [ 57 ].…”

Section: Structural Characterization Of Se‑polysaccharidesmentioning

confidence: 99%

“…Gao et al [ 57 ] found that synthetic Se-polysaccharides with a low molecular weight (11.00 kDa) exhibited superior antioxidant activity than native non-Se-polysaccharides with high molecular weight (15.80 kDa). Unfortunately, there is still a lack of direct evidence for the relationship between molecular weight and hypoglycemic activity of Se-polysaccharides.…”

Section: Structure–activity Relationship Of Se-polysaccharidesmentioning

confidence: 99%

Chemical Structure, Hypoglycemic Activity, and Mechanism of Action of Selenium Polysaccharides

Duan

Yang

Zhang

et al. 2021

Biol Trace Elem Res

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“…Unfortunately, the natural selenium-polysaccharide is scarce in nature. To efficiently harvest selenium-polysaccharide, continuous attention has been paid to the chemical synthesis of selenium-polysaccharide [19][20][21]. Compared with that of polysaccharide, the microstructure of selenium-polysaccharide may be changed.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and antioxidant activity in vitro of selenium-Euryale ferox Salisb. polysaccharide

et al. 2021

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In order to obtain and explore selenide composed of selenium and polysaccharide, three parameters were selected to optimize the synthesis process of selenium-Euryale ferox Salisb. polysaccharide (Se-ESPS) by Box-Behnken design. Furthermore, ESPS-B1 separated from ESPS was selenitized to Se-ESPS-B1 by the optimal synthesis process, then the characterization, and antioxidant activity in vitro of Se-ESPS-B1 were explored. The result manifested that the Se content of Se-ESPS was (2.915 ± 0.03) mg/g according to the optimal synthesis process of Se-ESPS (reaction time at 5 h, reaction temperature at 81 ℃, weight ratio of Na2SeO3 to ESPS at 0.9 g/g). A series of detection results indicated that the characterizations of Se-ESPS-B1 were apparently distinguished from that of ESPS-B1. Moreover, the antioxidant experiments in vitro demonstrated that Se-ESPS-B1 could exert antioxidant activity by scavenging DPPH, ABTS,·OH, and increasing reduction ability. In conclusion, the synthesis process is an effective approach to harvest selenium-polysaccharide, and Se-ESPS-B1 may be utilized as a potential antioxidant or selenium nutritional supplement.

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Structural features, selenization modification, antioxidant and anti-tumor effects of polysaccharides from alfalfa roots

Cited by 59 publications

References 42 publications

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

Chemical Structure, Hypoglycemic Activity, and Mechanism of Action of Selenium Polysaccharides

Synthesis, characterization, and antioxidant activity in vitro of selenium-Euryale ferox Salisb. polysaccharide

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