In this work, ZnO thin films grown on heated glass substrates in a temperature range of 300 to 500 °C with a 50°C step. The prepared solution is composed of methanol and zinc acetate Zn(CH3COO)2.2H2O. ZnO thin films are deposited by pyrolysis spray technique, our work focuses on the study of the substrate temperature influence on the structural and optical properties of these layers. Therefore, The X-ray diffraction, showed a Wurtzit hexagonal structure of elaborated films, with (002) as a preferred orientation, and a grain size of 64 to 74 nm. The optical transmission spectroscopy UV-Visible, illustrated an increase of optical band gap from 3.19 to 3.25 eV, proportionally with the substrate temperature.