Textile-based strain sensors are a potential platform used in wearable devices for sensing and. 8 sensors containing monitoring the human body. These sensors not only have all the conventional sensors benefits but also, they are low-cost, flexible, light-weight, and easily adopted with three-dimensional shape of the body. Moreover, recent research has shown they are the best candidates for monitoring human’s body motion. In this study, the effect of tensile fatigue cyclic loads on performance and sensitivity of textilebased strain sensors was investigated polyester/stainless steel staple fiber blend yarn as a conductive part with different structures were produced. The sensors varied in weft and warp density, percentage of stainless steel in conductive yarn, the number of conductive yarns, and weave pattern. The sensors were subjected to 500 cyclic loads operations and their tensile properties and sensitivity were investigated and compared before and after applying tensile fatigue cyclic loads. The results showed the textile-based strain sensors containing less percentage of stainless-steel fiber, lower number of conductive yarns, twill weave pattern and lower density in warp and weft direction have shown better performance after tensile fatigue cyclic loads.