Structure activity relationship studies on cytotoxicity and the effects on steroid receptors of AB-functionalized cholestanes

Novel alkylaminoethyl derivatives of androstane 3-oximes as anticancer candidates: synthesis and evaluation of cytotoxic effects

“…The first, MTT test with tetrazolium salt, 41 as described in detail by Ajduković et al 26 was performed on MCF-7, MDA-MB-231, PC-3, HeLa, HT-29, A549 and MRC-5 cell lines, with five different concentrations of the tested compounds ranging from 0.01 to 100 μM. The second cytotoxicity assay using resazurin 42 (manufacturer's protocol – Sigma Aldrich, St. Louis, MO, USA), was performed as described earlier for protocol with Calcein AM dye 44 on CEM, G-361 and BJ cell lines, with six concentrations of tested compounds ranging from 0.2 to 50 μM (dilution 3×). The data shown are the IC 50 values calculated from the means of results obtained from at least two or three independent experiments performed in triplicate or quadruplicate.…”

“…Enzyme-linked activity assay (ELISA) was used to detect the levels of cell adhesion molecule ELAM on HUVECs after 30 min of incubation with tested compounds and 4 h of stimulation with TNFα as described earlier. 44 Curcumin was used as a positive control. Experiments were repeated three times in triplicate.…”

Synthesis of New Cisplatin Derivatives from Bile Acids

“…IC 50 values (50% inhibitory concentrations) were calculated as the proportions of surviving cells. A detailed procedure for the cytotoxicity assay was described previously, except for using Alamar Blue dye instead of Calcein AM [37]. Briefly, the percentage of surviving cells in each well was calculated by dividing the intensity of the fluorescence signals from the exposed wells by the intensity of signals from control wells and multiplying by 100.…”

Synthesis and Biological Activity of Brassinosteroid Analogues with a Nitrogen-Containing Side Chain

“…BRI1 has been established as an authentic brassinosteroid receptor through genetic and biochemical investigations [10]. Moreover, recent studies have shown that natural BRs and their synthetic analogues have potential application not only in agriculture, but also in medicine due to their antiviral [11,12], immunomodulatory and neuroprotective activities [13][14][15], and anti-proliferative effects on animal cells in vitro [16][17][18][19][20].…”

“…Sci. 2021, 22, x FOR PEER REVIEW 2 of 28 their antiviral [11,12], immunomodulatory and neuroprotective activities [13][14][15], and anti-proliferative effects on animal cells in vitro [16][17][18][19][20]. Since the first BR isolation and structural identification, chemists began to synthesize new brassinosteroid analogues with different modifications of the sterol skeleton and tested them on various biological activities, especially in plant bioassays.…”