Structure of aldolase A (muscle-type) cDNA and its regulated expression in oocytes, embryos and adult tissues of Xenopus laevis

Hikasa, Hiroki; Hori, Katsuji; Shiokawa, K.

doi:10.1016/s0167-4781(97)00086-9

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…Such a switch from the maternal to zygotic mRNA was also observed for aldolases A and C. The maternal aldolase B mRNA occurs only at a residual level and extensive transcription takes place only after the late neurula stage. This agrees with the the zymogram analysis: Predominant enzymes present in early embryos were aldolase A and C [25]. We confirmed that the tissue-specific expression pattern in embryos was mostly as in adults.…”

Section: Resultssupporting

confidence: 91%

“…Tissue-specific expression of aldolase genes in embryos According to whole mount in situ hybridization, the earliest stage at which we detected localized signals for aldolase A, B and C mRNA was stage 16 (early neurula stage), 25 (late-neurula stage), and 21 (neurula stage), respectively [25], [26]. Throughout later stages, the aldolase A mRNA signal was noted in somites.…”

Section: Expression Of Three Aldolase Mrnas In Oocytes and Embryosmentioning

confidence: 78%

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A developmental biological study of aldolase gene expression in Xenopus laevis

et al. 2002

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We cloned cDNAs for Xenopus aldolases A, B and C. These three aldolase genes are localized on different chromosomes as a single copy gene. In the adult, the aldolase A gene is expressed extensively in muscle tissues, whereas the aldolase B gene is expressed strongly in kidney, liver, stomach and intestine, while the aldolase C gene is expressed in brain, heart and ovary. In oocytes aldolase A and C mRNAs, but not aldolase B mRNA, are extensively transcribed. Thus, aldolase A and C mRNAs, but not B mRNA, occur abundantly in eggs as maternal mRNAs, and strong expression of aldolase B mRNA is seen only after the late neurula stage. We conclude that aldolase A and C mRNAs are major aldolase mRNAs in early stages of Xenopus embryogenesis which proceeds utilizing yolk as the only energy source. aldolase B mRNA, on the other hand, is expressed only later in development in tissues which are required for dietary fructose metabolism. We also isolated the Xenopus aldolase C genomic gene (ca. 12 kb) and found that its promoter (ca. 2 kb) contains regions necessary for tissue-specific expression and also a GC rich region which is essential for basal transcriptional activity.

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Section: Resultssupporting

confidence: 91%

Section: Expression Of Three Aldolase Mrnas In Oocytes and Embryosmentioning

confidence: 78%

A developmental biological study of aldolase gene expression in Xenopus laevis

et al. 2002

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“…Aldolase is an important enzyme in glycolysis; the three vertebrate homologs have been cloned in humans (Besmond et al, 1983), mice (Tsutsumi et al, 1983(Tsutsumi et al, , 1984, and Xenopus (Atsuchi et al, 1994;Hikasa et al, 1997;Kajita et al, 2001). In the present study, we isolated the aldolase A, B, and C genes in grass carp and characterized their expression.…”

Section: Discussionmentioning

confidence: 80%

“…Besides, upregulation of the aldolase A and C genes and downregulation of aldolase B gene have been observed in the hepatic tissue of liver cancer patients (Schapira et al, 1963). Aldolase A, B, and C genes have been isolated from amphibian species such as Xenopus laevis (Atsuchi et al, 1994;Hikasa et al, 1997;Kajita et al, 2001) and their spatial and temporal expression patterns have been characterized (Kajita et al, 2001). The aldolase B gene in fish has been cloned in bream (Llewellyn et al, 1995) and salmon (Llewellyn et al, 1998), while the aldolase C gene has been cloned in goldfish (Berardini et al, 1997); however, there have been no reports on the cloning of all three aldolase genes in a single fish species.…”

Section: Introductionmentioning

confidence: 99%

Research Article Tissue distribution and early developmental expression patterns of aldolase A, B, and C in grass carp Ctenopharyngodon idellus.

Fan¹,

Bai²,

Ma³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model owing to its relatively high glucosemetabolizing capability. Based on the cDNA sequences of the aldolase A, B, and C genes, the expression patterns of these three isozymes were analyzed in different tissues and during early embryogenesis using quantitative real-time polymerase chain reaction (qRT-PCR). Sequence analysis of cDNAs indicated that aldolase A, B, and C (GenBank accession numbers: KM192250, KM192251, and KM192252) consist of 364, 364, and 363 amino acids, respectively. The qRT-PCR results showed that the expression levels of aldolase A, B, and C were highest in the muscle, liver, and brain, respectively. Aldolase A and C exhibited similar expression patterns during embryogenesis, with high levels observed in unfertilized and fertilized eggs and at the blastocyst stage, 2 J.J. Fan et al.Genetics and Molecular Research 16 (3): gmr16039234 followed by a decline and then increase after organogenesis. In contrast, aldolase B transcript was not detected during the unfertilized egg stage, and appeared only from gastrulation; the expression increased markedly during the feeding period (72 h after hatching), at which point the level was higher than those of aldolase A and C. These data suggest that the glucose content of grass carp starter feed should be adjusted according to the metabolic activity of aldolase B.

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“…A lysine residue (K229) forming a putative Shiff base is represented by a bold character on a black background. The aldolase isozymes are as follows: human aldolase A (P04075, Sakakibara et al 1985), Xenopus laevis A (BAA19524, Hikasa et al 1997), lamprey muscle type (M) (P53445, Zhang et al 1995), Drosophila melanogaster Dm a (JX0233, Kai et al 1992), Caenorhabditis elegans Ce-1 (P54216, Inoue et al 1997), Onchocerca volvulus (AAD38430, McCarthy et al 2002), E. multilocularis (CAC18550, Brehm et al 2000), and S. mansoni (AAA57567, El-Dabaa et al 1998) of C. sinensis FBP aldolases, a lysine residue is positioned at the 6-phosphate binding site and a histidine residue is located at 361 (CsFbA-1) and 362 (CsFbA-2 and -3), suggesting that these three putative isoenzymes could be assigned as FBP aldolase type A enzymes. In this study, a large number of ESTs was collected from C. sinensis and an EST platform was formulated as an aid to future research at the multigene level.…”

Section: Discussionmentioning

confidence: 99%

Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke

et al. 2006

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Expressed sequence tag (EST) pools represent partial profiles of the gene expressions of organisms. In an effort to construct a Clonorchis sinensis EST pool, 2,387 ESTs were collected from an adult C. sinensis cDNA library and assembled into 1,573 clusters. Of these clusters, 1,225 ESTs (51%) were singletons and 348 clusters consisted of more than two ESTs. There were 848 clusters (54%) that shared significant identity with previously reported proteins, and of these, 401 clusters were categorized into 11 major functional protein classes. Three cDNA clones of fructose-1,6-bisphosphate (FBP) aldolase were selected from the C. sinensis EST pool and analyzed for phylogenic clustering. FBP clones encoded a complete polypeptide, which shared significant identity to those of vertebrate and invertebrate animals and clustered with those of trematodes. We believe that the EST pool described can be confidently used as a platform in multigene researches on C. sinensis gene expression.

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Structure of aldolase A (muscle-type) cDNA and its regulated expression in oocytes, embryos and adult tissues of Xenopus laevis

Cited by 8 publications

References 37 publications

A developmental biological study of aldolase gene expression in Xenopus laevis

A developmental biological study of aldolase gene expression in Xenopus laevis

Research Article Tissue distribution and early developmental expression patterns of aldolase A, B, and C in grass carp Ctenopharyngodon idellus.

Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke

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