Structure of biomimetic apatite grown on hydroxyapatite (HA)

Vargas-Becerril, Nancy; Sánchez-Téllez, Daniela Anahí; Zarazúa-Villalobos, L.; González-García, Dulce María; Álvarez-Pérez, Marco Antonio; León-Escobedo, C. de; Téllez-Jurado, Lucía

doi:10.1016/j.ceramint.2020.08.044

Cited by 47 publications

(18 citation statements)

References 46 publications

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“…Compared to the samples sintered in an air atmosphere, the composites sintered in an Ar atmosphere exhibit a higher Ca/P atomic ratio. The results of Figures and demonstrate an enhanced apatite-inducing ability for composites sintered in Ar, which might be attributed to enhanced wettability and finer particle size …”

Section: Resultsmentioning

confidence: 84%

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Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Cai

Wang

et al. 2021

ACS Omega

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The effect of sintering atmosphere (air and Ar) and temperature (1100, 1200, 1300 °C) on the microstructure, mechanical properties, and bioactivity of hydroxyapatite/tantalum (HA/Ta) composites were systematically investigated by pressureless sintering of the mixture of HA and Ta powders. It shows that the sintering atmosphere greatly impacts the phase composition and microstructure of the HA/Ta composites. The higher diffusion of atoms promotes shrinkage and causes deeper reaction fusion between the HA matrix and Ta, which improved the interfacial binding of the HA/Ta composites. The refined grain structure and improved interfacial binding obtained within the Ar atmosphere compared to the air atmosphere benefit the mechanical properties. The maximum bending strength and shrinkage observed for the composites sintered at 1300 °C in the Ar atmosphere are 27.24 MPa and 6.65%, respectively. The cell counting kit-8 (CCK-8) method was used to investigate the in vitro cytocompatibility of HA/Ta composites. The results revealed that the HA/Ta composites sintered with different conditions have no cytotoxicity. The simulated body fluid (SBF) soaking results showed that all of the studied composites possess desirable bioactivity, as demonstrated by their ability to form calcium-deficient carbonate apatite layer on the surfaces. For composites sintered at 1300 °C, the surface apatite layer coverage of the composites obtained in the Ar atmosphere was increased by 139.7% than the ones obtained in air, which confirmed an enhanced bioactive mineralization ability. The results indicated that the HA/Ta composites sintered at 1300 °C in Ar possess desirable mechanical properties and bioactivity. This work opens up the new possibility for preparing HA-based composites and is of great value in biomedical applications.

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Section: Resultsmentioning

confidence: 84%

“…The results of Figures 11 and 12 demonstrate an enhanced apatite-inducing ability for composites sintered in Ar, which might be attributed to enhanced wettability and finer particle size. 37…”

Section: → + +mentioning

confidence: 99%

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Cai

Wang

et al. 2021

ACS Omega

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“…XRD analysis showed that the main chemical composition of BpNcCaP was hexagonal HA according to the ICSD-PDF card 86-0740. The unit cell parameters (a = b = 0.946 nm and c = 0.687 nm) of BpNcCaP resembled the characteristic parameters of HA (Vargas-Becerril et al, 2020). In conventional methods to fabricate granular bone-defect-filling materials, sintering is always adopted to increase the grain size, during which the crystallite size of HA also dramatically increases to 45-55 nm (Rodríguez-Lugo et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Development, In-Vitro Characterization and In-Vivo Osteoinductive Efficacy of a Novel Biomimetically-Precipitated Nanocrystalline Calcium Phosphate With Internally-Incorporated Bone Morphogenetic Protein-2

Shen²,

Lin³

et al. 2022

Front. Bioeng. Biotechnol.

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The repair of large-volume bone defects (LVBDs) remains a great challenge in the fields of orthopedics and maxillofacial surgery. Most clinically available bone-defect-filling materials lack proper degradability and efficient osteoinductivity. In this study, we synthesized a novel biomimetically-precipitated nanocrystalline calcium phosphate (BpNcCaP) with internally incorporated bone morphogenetic protein-2 (BpNcCaP + BMP-2) with an aim to develop properly degradable and highly osteoinductive granules to repair LVBDs. We first characterized the physicochemical properties of the granules with different incorporation amounts of BMP-2 using scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. We evaluated the cytotoxicity and cytocompatibility of BpNcCaP by assessing the viability and adhesion of MC3T3-E1 pre-osteoblasts using PrestoBlue assay, Rhodamine-Phalloidin and DAPI staining, respectively. We further assessed the in-vivo osteoinductive efficacy in a subcutaneous bone induction model in rats. In-vitro characterization data showed that the BpNcCaP + BMP-2 granules were comprised of hexagonal hydroxyapatite with an average crystallite size ranging from 19.7 to 25.1 nm and a grain size at 84.13 ± 28.46 nm. The vickers hardness of BpNcCaP was 32.50 ± 3.58 HV 0.025. BpNcCaP showed no obvious cytotoxicity and was favorable for the adhesion of pre-osteoblasts. BMP-2 incorporation rate could be as high as 65.04 ± 6.01%. In-vivo histomorphometric analysis showed that the volume of new bone induced by BpNcCaP exhibited a BMP-2 amount-dependent increasing manner. The BpNcCaP+50 μg BMP-2 exhibited significantly more degradation and fewer foreign body giant cells in comparison with BpNcCaP. These data suggested a promising application potential of BpNcCaP + BMP-2 in repairing LVBDs.

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“…Therefore, it is often used as a bone graft substitute in biomedical clinics [ 7 ]. Different forms of apatite with different sizes and shapes (e.g., flakes, spheres, needles, and rods) have been fabricated using wet and dry synthesis strategies [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. HA can be synthesized by a precipitation method [ 8 , 9 ], sol-gel method [ 10 , 11 ], hydrothermal method [ 8 , 9 , 10 ] and microwave-assisted synthesis method [ 15 , 16 ], or it can be directly extracted from biological resources [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Different forms of apatite with different sizes and shapes (e.g., flakes, spheres, needles, and rods) have been fabricated using wet and dry synthesis strategies [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. HA can be synthesized by a precipitation method [ 8 , 9 ], sol-gel method [ 10 , 11 ], hydrothermal method [ 8 , 9 , 10 ] and microwave-assisted synthesis method [ 15 , 16 ], or it can be directly extracted from biological resources [ 17 , 18 , 19 ]. Reactions during hydrothermal wet precipitation can be effectively controlled by adjusting the reaction medium, pH value, and concentration at different temperatures, amounts of pressure, and reaction times [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological Changes, Antibacterial Activity, and Cytotoxicity Characterization of Hydrothermally Synthesized Metal Ions-Incorporated Nanoapatites for Biomedical Application

et al. 2022

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The objective of this study was to prepare hydroxyapatite (HA) with potential antibacterial activity against gram-negative and gram-positive bacteria by incorporating different atomic ratios of Cu2+ (0.1–1.0%), Mg2+ (1.0–7.0%), and Zn2+ (1.0–7.0%) to theoretically replace Ca2+ ions during the hydrothermal synthesis of grown precipitated HA nanorods. This study highlights the role of comparing different metal ions on synthetic nanoapatite in regulating the antibacterial properties and toxicity. The comparisons between infrared spectra and between diffractograms have confirmed that metal ions do not affect the formation of HA phases. The results show that after doped Cu2+, Mg2+, and Zn2+ ions replace Ca2+, the ionic radius is almost the same, but significantly smaller than that of the original Ca2+ ions, and the substitution effect causes the lattice distance to change, resulting in crystal structure distortion and reducing crystallinity. The reduction in the length of the nanopatites after the incorporation of Cu2+, Mg2+, and Zn2+ ions confirmed that the metal ions were mainly substituted during the growth of the rod-shape nanoapatite Ca2+ distributed along the longitudinal site. The antibacterial results show that nanoapatite containing Cu2+ (0.1%), Mg2+ (3%), and Zn2+ (5–7%) has obvious and higher antibacterial activity against gram-positive bacteria Staphylococcus aureus within 2 days. The antibacterial effect against the gram-negative bacillus Escherichia coli is not as pronounced as against Staphylococcus aureus. The antibacterial effect of Cu2+ substituted Ca2+ with an atomic ratio of 0.1~1.0% is even better than that of Mg2+- and Zn2+- doped with 1~7% groups. In terms of cytotoxicity, nanoapatites with Cu2+ (~0.2%) exhibit cytotoxicity, whereas Mg2+- (1–5%) and Zn2+- (~1%) doped nanoapatites are biocompatible at low concentrations but become cytotoxic as ionic concentration increases. The results show that the hydrothermally synthesized nanoapatite combined with Cu2+ (0.2%), Mg2+ (3%), and Zn2+ (3%) exhibits low toxicity and high antibacterial activity, which provides a good prospect for bypassing antibiotics for future biomedical applications.

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Structure of biomimetic apatite grown on hydroxyapatite (HA)

Cited by 47 publications

References 46 publications

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Fabrication of Hydroxyapatite/Tantalum Composites by Pressureless Sintering in Different Atmosphere

Development, In-Vitro Characterization and In-Vivo Osteoinductive Efficacy of a Novel Biomimetically-Precipitated Nanocrystalline Calcium Phosphate With Internally-Incorporated Bone Morphogenetic Protein-2

Morphological Changes, Antibacterial Activity, and Cytotoxicity Characterization of Hydrothermally Synthesized Metal Ions-Incorporated Nanoapatites for Biomedical Application

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