Structure of isochorismate synthase DhbC fromBacillus anthracis

Domagalski, Marcin J.; Tkaczuk, Karolina L.; Chruszcz, M.; Skarina, T.; Onopriyenko, O.; Cymborowski, M.; Grabowski, M.; Savchenko, Alexei; Minor, Władek

doi:10.1107/s1744309113021246

Cited by 3 publications

(2 citation statements)

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“…The function and structure of asbB (Nusca et al, 2012), asbD (Schmelz et al, 2009) and asbF (Pfleger et al, 2008) in Petrobactin siderophore synthesis gene cluster have been investigated. Domagalski et al (2013) determined the crystal structure of different chorismic acid synthetase DhbC of Bacillibactin siderophore in Bacillus anthraci. DhbC participated in the biosynthesis of 2, 3-two hydroxybenzoate (DHB) and methyl naphthoquinone (MK) of the respiratory chain group; the consumption of DhbC led to the deficiency of DHB, which showed the importance of DhbC in siderophore biosynthesis (Domagalski et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Domagalski et al (2013) determined the crystal structure of different chorismic acid synthetase DhbC of Bacillibactin siderophore in Bacillus anthraci. DhbC participated in the biosynthesis of 2, 3-two hydroxybenzoate (DHB) and methyl naphthoquinone (MK) of the respiratory chain group; the consumption of DhbC led to the deficiency of DHB, which showed the importance of DhbC in siderophore biosynthesis (Domagalski et al, 2013). Hertlein et al (2014) studied the deletion of DhbF by gene inactivation mutagenesis and confirmed that dhb gene cluster was in charge of the synthesis of Bacillibactin siderophore (Hertlein et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

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Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Sheng¹,

Jia²,

Xiaomai³

et al. 2018

American Journal of Biochemistry and Biotechnology

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Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type and antibacterial effects. The method is using genome mining technology to analyze the siderophore synthesis genes and the phylogenetic tree of each synthesis gene was constructed separately. Iron free medium was utilized to induce the synthesis of siderophore and CAS liquid detection method was used for qualitative and quantitative analysis on siderophore. The type of siderophore was preliminaries identified by Arnow and its antibacterial effects were analyzed according to the agar punching method. The results show that a siderophore synthesis gene cluster with 83% similarity to Petrobactin was found in Brevibacillus brevis GZDF3 genome. Iron free medium could induce siderophore synthesis and the optimal incubation time cultured in iron free medium was 30 h and 48 h. Antagonistic strain GZDF3 had the capacity to synthesize catechol-type siderophore. Also, GZDF3 had a powerful antibacterial effect on pathogenic fungus Fusarium oxysporum of rotted root on Pinellia ternata. Therefore, Brevibacillus brevis GZDF3 can produce catechol-type siderophore in an iron-deficient culture medium, which was also a main antifungal active substance.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Sheng¹,

Jia²,

Xiaomai³

et al. 2018

American Journal of Biochemistry and Biotechnology

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Structure of subunit I of the anthranilate synthase complex of Mycolicibacterium smegmatis

Chen

Jia

Liu

et al. 2020

Biochemical and Biophysical Research Communications

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Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato

Pengproh,

Thanyasiriwat,

Sangdee

et al. 2023

Plant Pathol J

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Recently, strategies for controlling Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of Fusarium wilt of tomato, focus on using effective biocontrol agents. In this study, an analysis of the biocontrol and plant growth promoting (PGP) attributes of 11 isolates of loamy soil Bacillus spp. has been conducted. Among them, the isolates B.PNR1 and B.PNR2 inhibited the mycelial growth of Fol by inducing abnormal fungal cell wall structures and cell wall collapse. Moreover, broad-spectrum activity against four other plant pathogenic fungi, F. oxysporum f. sp. cubense race 1 (Foc), Sclerotium rolfsii, Colletotrichum musae, and C. gloeosporioides were noted for these isolates. These two Bacillus isolates produced indole acetic acid, phosphate solubilization enzymes, and amylolytic and cellulolytic enzymes. In the pot experiment, the culture filtrate from B.PNR1 showed greater inhibition of the fungal pathogens and significantly promoted the growth of tomato plants more than those of the other treatments. Isolate B.PNR1, the best biocontrol and PGP, was identified as Bacillus stercoris by its 16S rRNA gene sequence and whole genome sequencing analysis (WGS). The WGS, through genome mining, confirmed that the B.PNR1 genome contained genes/gene cluster of a nonribosomal peptide synthetase/polyketide synthase, such as fengycin, surfactin, bacillaene, subtilosin A, bacilysin, and bacillibactin, which are involved in antagonistic and PGP activities. Therefore, our finding demonstrates the effectiveness of B. stercoris strain B.PNR1 as an antagonist and for plant growth promotion, highlighting the use of this microorganism as a biocontrol agent against the Fusarium wilt pathogen and PGP abilities in tomatoes.

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Structure of isochorismate synthase DhbC fromBacillus anthracis

Cited by 3 publications

References 47 publications

Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Structure of subunit I of the anthranilate synthase complex of Mycolicibacterium smegmatis

Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato

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