Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe 31 )/selenate (SeO 22 4 ) ions into the HA structure (Ca 10-x-y Fe y (PO 4 ) 6-x (SeO 4 ) x (OH) 2-x-y O y ) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 9008C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe 31 amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe 31 concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe 31 /SeO 22 4 ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe 31 concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB)