Studies of Acetal Phospholipides of Brain

Thannhauser, S. J.; Boncoddo, Nicholas F.; Schmidt, Gerhard

doi:10.1016/s0021-9258(18)56184-6

Cited by 58 publications

(2 citation statements)

References 8 publications

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“…The insoluble tissue residue from chloroform-methanol extraction is contaminated with lipides and proteolipides present in the aliquot of solvent with which it is 2 Our thanks are due to Dr. G. Schmidt, of the Department of Biochemistry, Tufts College Medical School, Boston, who has kindly allowed us access to his unpublished procedure for this determination. The method has been standardized by Dr. Schmidt with pure brain acetal phosphatide prepared by Tha~nhauser et al (29). wet.…”

Section: Methodsmentioning

confidence: 99%

A Study of the Chemical Nature of Components of Bovine White Matter Effective in Producing Allergic Encephalomyelitis in the Rabbit

Waksman

Porter

Lees

et al. 1954

Journal of Experimental Medicine

112

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Experimental allergic encephalomyelitis (EAE) is a well defined, reproducible entity based on sensitivity to autologous tissue antigen (for recent reviews, see references 1-3). Its importance as a model for certain forms of demyelinating disease in man has made it a subject of interest to investigators from a number of unrelated disciplines. However, immunologic and other investigations of the disease mechanism have been seriously handicapped by lack of information regarding the exact chemical nature of the encephalitogenic antigen or antigens in nervous tissue. The present paper presents for the first time precise chemical data regarding fractionated materials which show antigenic activity.The tissues of the central nervous system contain several distinct substances with the capacity to induce circulating antibody formation (4). Many of these substances, like the antigen(s) responsible for the production of EAE, are organ-rather than species-specific. Their exact chemical nature, however, even after 30 years of investigation, remains obscure. A few simple lipides have been reported to possess antigenicity even when produced synthetically. It is not known if any of these substances are encephalitogenic when administered with adjuvants, although some negative data have been obtained on this point (5).The earliest studies of EAE indicated that the encephalitogenie activity of nervous tissue is largely confined to those parts that axe rich in white matter (6, 7), and is absent from fetal or unmyelinated brain (7,8). As nervous tissue of foreign species is effective in producing EAE (7-11) the antigenicity is said to be organ-specific rather than species-specific. It has been reported that activity is associated with

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Section: Methodsmentioning

confidence: 99%

A Study of the Chemical Nature of Components of Bovine White Matter Effective in Producing Allergic Encephalomyelitis in the Rabbit

Waksman

Porter

Lees

et al. 1954

Journal of Experimental Medicine

112

View full text Add to dashboard Cite

show abstract

“…The ion exchange resins have become of considerable importance in the study, isolation, and recovery of several biological and biochemical substances: proteins (87,157,238,846,420) The use of ion exchange techniques in analytical chemistry continues to grow quite rapidly. Reviews of this application have been contributed by Futterknecht (147), Honda (193), Wickbold (452), Davies (99), and Stein and Moore (404).…”

Section: Biochemistrymentioning

confidence: 99%