Studies of deionization and impedance spectroscopy for blood analyzer

Kwong, C.C.H.; Li, Nan; Ho, Chih‐Ming

doi:10.1117/12.637567

Cited by 5 publications

(1 citation statement)

References 12 publications

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“…Also, a micro cell Coulter counter, which has already been demonstrated in our lab [17], will be incorporated into current device to count the separated cells. …”

Section: Discussionmentioning

confidence: 99%

On-Chip Continuous Blood Cell Subtype Separation by Deterministic Lateral Displacement

Kamei

2007

2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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This paper presents a microfluidic device for continuous human blood cell subtype separation using the deterministic lateral displacement principle. Based on their significant size and shape differences, three major cell types of human whole blood -platelets, red blood cells and white blood cells -were demonstrated to be directly separated using a twostage separation strategy. Even though all white blood cells are spherical and have diameters within a narrow range (8 -20pm), the initial limitation for using this principle to separate white blood cell subtypes was conquered by attaching larger polystyrene microbeads to one of the subtypes to amplify the size differences. Specifically, continuous separation of human CD4+ T helper lymphocytes from other white blood cell subtypes was achieved with high purity and recovery due to the underlying high affinity and high specificity of the antigen-antibody interaction used to attach the microbeads to the lymphocytes.With our novel approach, the pure population of one blood cell subtype can be effectively isolated by exploring the deterministic lateral displacement principle, which has the advantages of the simplicity, high speed and high resolution. Because many cells express unique surface markers, this method can theoretically be applied to separate any target cell type from a heterogeneous mixture for downstream analysis.

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“…Also, a micro cell Coulter counter, which has already been demonstrated in our lab [17], will be incorporated into current device to count the separated cells. …”

Section: Discussionmentioning

confidence: 99%

On-Chip Continuous Blood Cell Subtype Separation by Deterministic Lateral Displacement

Kamei

2007

2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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Micro Electrical Impedance Spectroscopy (μEIS) Fabricated on the Curved Surface of a Fine Needle for Biotissue Discrimination

et al. 2015

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This work presents a novel micro electrical impedance spectroscopy (μEIS) technique that can measure and discriminate the electrical signal responses of biotissues in real time. An EoN (EIS‐on‐a‐needle), EIS on the surface of a fine needle (400 μm in diameter), was fabricated using a newly developed flexible photomask film. The base material of the photomask is parylene‐C, which allows uniform contact on the curved surface of the needle; thus, the designed electrode patterns of the photomask can be transferred onto the needle surface with a high resolution (2.95 % or less in dimensional error). To validate the developed EoN as an electrical sensor, ex vivo experiments with various biotissues—butchered pork (skin, fat, and muscle) and human breast tissues (normal and cancerous)—were conducted by measuring real‐time electrical impedance during a frequency sweep. The conductivities (relative permittivity) of the pork tissues were evaluated by electrical equivalent circuit analysis: 56.6 mS/m (37,800), 68.0 mS/m (74,755), and 74.9 mS/m (26,145) for the skin, fat, and muscle, respectively. Moreover, the normal and cancerous tissues were well distinguished by electrical resistance at 4.04 kHz for various cancer grades (Elston grades 1, 2, and 3). Analysis of the electrical impedance suggests that the EoN can be utilized to diagnose the physiological states of biotissues in clinical use.

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All electronic approach for high-throughput cell trapping and lysis with electrical impedance monitoring

Ameri¹,

Singh²,

Dokmeci³

et al. 2014

Biosensors and Bioelectronics

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Studies of deionization and impedance spectroscopy for blood analyzer

Cited by 5 publications

References 12 publications

On-Chip Continuous Blood Cell Subtype Separation by Deterministic Lateral Displacement

On-Chip Continuous Blood Cell Subtype Separation by Deterministic Lateral Displacement

Micro Electrical Impedance Spectroscopy (μEIS) Fabricated on the Curved Surface of a Fine Needle for Biotissue Discrimination

All electronic approach for high-throughput cell trapping and lysis with electrical impedance monitoring

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