Studies of the CETP TaqIB and ApoE Polymorphisms in Southern Thai Subjects with the Metabolic Syndrome

Abstract

Several genetic factors have been investigated responsible for metabolic syndrome (MetS). The aim of this study was to investigate the association between cholesteryl ester transfer protein (CETP) TaqIB and apolipoprotein E (ApoE) polymorphisms and MetS in 378 subjects from Southern Thailand. Subjects were divided into MetS+ (n = 121) and MetS- (n = 257) groups according to the criteria of National Cholesterol Education Program Adult Treatment Panel III (NCEP ATPIII). The CETP TaqIB and ApoE polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Logistic regression analysis revealed no association of CETP TaqIB and ApoE variants with MetS, after adjustment for age and sex. However, ε4 allele had a significantly increased odds ratio (OR) of reduced high-density lipoprotein-cholesterol (HDL-C) levels when compared with ε3 allele (OR 1.91; 95% CI 1.11-3.29, p = 0.020). This suggests that CETP TaqIB and ApoE polymorphisms may not be considered as genetic risk factors for MetS in a Southern Thai population. However, ε4 allele which is associated with one metabolic component, low HDL-C levels, might predispose the subjects to develop metabolic disturbances.

Association between Six CETP Polymorphisms and Metabolic Syndrome in Uyghur Adults from Xinjiang, China

“…This is consistent with Anton et al [36] who hold that the risk of MS was reduced by 32% ( p = 0.005, OR = 0.68) in carriers of the B 2 variant. However, this was different from observations by Jeenduang [38] and Maroufi [39]. …”

“…The rs12149545-A frequency was 29.1%, and the rs711752-A frequency (46.8%) was similar to that in Latvians (46.2%) [21]. For rs708272, the rs708272-T frequency (46.8%) was higher than that in Austria (41.3) [36], Turkey (43%) [37], or Southern Thailand (37.43%) [38]. Moreover, rs708272-T plays an inactive role in the development of MS.…”

The effect of APOE, CETP, and PCSK9 polymorphisms on simvastatin response in Thai hypercholesterolemic patients

“…We found that the allele frequencies of CETP TaqIB polymorphisms (B1 45.78%, B2 54.22%) in this study were similar to the Indian (B1 41.8%, B2 58.2%) population. In contrast, our results were different from Thai (B1 62.57%, B2 37.43%), Chinese (B1 62.8%, B2 37.2%), and Japanese (B1 62%, B2 38%) populations . This suggests that our study population may be genetically different from the general population among Thais and other ethnic groups.…”

“…Previous studies have also reported that E4 allele is generally associated with increased plasma cholesterol and LDL‐C, and E2 allele is associated with decreased plasma cholesterol and LDL‐C compared with the E3 allele . The allele frequencies of the APOE polymorphisms (E2 7.11%, E3 79.56%, E4 13.33%) in this study were similar to previous reports among the Thai population (E2 7.18%‐10.42%, E3 77.04%‐77.51%, E4 12.54%‐15.43%) . APOE4 allele in our study was higher than Japanese (10.5%), Han Chinese (7.5%), Taiwanese (7.5%), and Korean (9%) populations but lower than the Malaysian population (20.20%) .…”

“…The quality and quantity of DNA were estimated using a NanoDrop 2000 UV‐Vis spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Genotyping of APOE (rs429358, rs7412); CETP TaqIB (rs708272); and PCSK9 R46L (rs11591147), I474V (rs562556), and E670G (rs505151) polymorphisms were performed using polymerase chain reaction‐restriction fragment length polymorphism (PCR–RFLP) as previously described …”