Studies on Behaviors of Interactions Between New Polymer-based ZIC-HILIC Stationary Phases and Carboxylic Acids

Abstract

Zwitterionic stationary phases with nearly identical capacities were prepared by graft polymerization of a series of sulfobetaine precursors onto the surface of porous PS/DVB particles. The different spacer lengths are used as an investigative tool for the retention behavior of carboxylic acids; namely malonic, succinic, glutaric and maleic acid. In zwitterionic ion chromatography-hydrophilic interaction liquid chromatography (ZIC-HILIC) separation mode, the retention characteristic of carboxylic acids was examined using sodium acetate/acetonitrile eluents and UV detection. The retention is based on partitioning in reversed as well as in HILIC mode and zwitterionic ion exchange resulting in a mixed separation mode for the carboxylic acids. This ion exchange behavior has never been observed before for sulfobetaine-based zwitterionic stationary phases.

A Study of Retention Behavior and Method Development of Salbutamol Sulfate in Hydrophilic Interaction Chromatography

“…The stationary phases used for the separation of SBS were performed onto the PS/DVB, using PEEK columns (100 × 4 mm ID) by means of grafted sulfobetaine monomers. [13][14][15][16] HPLC grade of ACN was purchased from Aldrich (total 99.93 percent). The capacity of the ZIC1 and ZIC5 columns are 432 and 488 μeq g -1 , 10 respectively.…”

“…Therefore, the linearization of the stationary phase functional groups, while ACN is present, is improved. 14 The pharmaceutical retention decreased or increased with an increasing buffer concentration in stationary ZIC-HILIC phases. The explanation for this is the exchange of cations and anions.…”

Investigate Retention Behavior of 2-deoxycytidine in Hydrophilic Interaction Liquid Chromatography

“…ZIC1 and ZIC5 were developed on the polystyrene-divinylbenzene (PS/DVB), by means of a grafted sulfobetaine monomer (100 × 4 mm ID) and PEEK column, for the 2-deoxycytidine separation. 5,[9][10][11] The systematic cycle of grafting reaction has been established by Raskop et al 20 Chemicals 2-deoxycytidine (HPLC) purchased by Sigma-Aldrich 99%. Acetic acid was purchased from BDH.…”

Development of a validated hydrophilic interaction chromatography method for the determination of cefotaxime in pharmaceutical preparations

“…Hydrophilic and polar compounds, as compared to hydrophobic neutral compounds, are retained preferentially in HILIC the contrary of RP-HPLC. Thus recently, HILIC technology has thus recently begun to increase dramatically in the estimation of drugs, nucleosides, carboxylic acids, inorganic ions, and amino acids by Rasheed and its co-workers [24][25][26][27][28][29][30][31][32][33][34]. Experimental…”