Studies on biomarkers of oxidative stress and associated genotoxicity and histopathology in Channa punctatus from heavy metal polluted canal

Javed, Mehjbeen; Ahmad, Irshad; Usmani, Nazura; Ahmad, Masood

doi:10.1016/j.chemosphere.2016.02.080

Cited by 111 publications

(57 citation statements)

References 44 publications

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“…8-OHdG urinary concentration is one of the most relevant effects of free radicalinduced oxidative lesions in DNA structure. This biomarker has been also used to evaluate DNA damage in humans after exposure to mutagenic agents, such as tobacco smoke, hydrocarbons derivatives and heavy metals which are known indeed to generate reactive oxygen species either in humans and inside the ecosystem [36][37][38][39]. In this respect, our study confirmed prior observations about a significant direct correlation between urinary concentration of 8-OHdG and composite heavy metal exposure [26].…”

Section: Discussionsupporting

confidence: 82%

Chelator in Environmentally-exposed Poor Detoxifiers: An Adjuvant Hepatoprotective Treatment for Poly-drug Users?

Marotta¹

2016

Mol Biol (Los Angel)

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Glutathione-S-Transferase (GST) genes, including GSTT1, GSTP1, and GSTM1, play a major role in detoxification and metabolism of xenobiotics. The aim of the present study was to test an orally bioavailable GSH-based compound in poor detoxifier subjects regarding their oxidative stress, ammonia metabolism and heavy metal clearance. Eligibility was established with a telephone questionnaire followed by a clinical examination to obtain height, weight, blood pressure and screening blood chemistry and GSTM-1 gene test. Seventy-five GSTM1-null, non-smoking healthy individuals were introduced in the study who were teetotallers or were using a maximum of 20 g alcohol/day men (n = 47) and women (n = 28) aged 38-69 years (mean: 49.7) with body-mass index ranging from 22 to 29 kg/m 2 ) participated in the study. All enrolled participants were free from acute or chronic diseases and did not eat a diet or take medications that might interfere with outcome markers namely antioxidant supplements, high intake of dietary polyphenols including coffee, grapefruit juice or anti-inflammatory medications. Subjects were divided in 3 groups (25 subjects each): a) Given an oral bioabsorbable glutathione-based compound added with selenium, L-cysteine and vitamin C (GLU-9599, Named Research Co., Lesmo, Italy) at the dosage of 1 tab a day for two months; b) 600 mg of glutathione in 250 saline, given intravenously over 90 min, twice a week for 2 months; and c) Given 3 g a day of a strong cation-exchange oral chelator consisting of a mixture of chabasite-phillipsite for two months. Dietary questionnaire was administered to all subjects. Either at baseline, at one month or at the end of the study the following parameters were tested: erythrocytes (RBC) level of GSH/GSSG and Glutathione Peroxidise (GPx), urinary 8-OHdG, 24 h urinary measurement of main heavy metals, faecal metals, ammonia and oxidative stress. GLU-9599 showed to significantly lower either RBC oxidative stress or urinary 8-OHdG as compared to either IV glutathione or oral chelator (p<0.01). There was a significant correlation between urinary 8-OHdG and overall heavy metal excretion (r: 0.58, p<0.005) but no correlation occurred with RBC oxidative stress. Few subjects with fatty liver and abnormal transaminases improved their value during treatment with GLU-9599. Only oral chelator was effective in significantly lower faecal metals, ammonia and faecal oxidative stress (p<0.005). There was a not significant trend increase of mercury faecal excretion and GLU-9599. Overall, it would appear that in poor-detoxifier (GSTM1-null genotype) subjects, an effective orally-absorbable stabilised-glutathione can efficiently restore antioxidant defences in view of a potential wider application as an integrative adjuvant co-treatment in patients environmentally-exposed and also under pharmacological burden. Effective heavy metal clearance remains a still unresolved issue which may benefit from the rational addition of high cation-exchange oral chelators with a likely association with via...

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Section: Discussionsupporting

confidence: 82%

Chelator in Environmentally-exposed Poor Detoxifiers: An Adjuvant Hepatoprotective Treatment for Poly-drug Users?

Marotta¹

2016

Mol Biol (Los Angel)

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“…This could be due to heavy metals and other domestic wastes which enter the pond. The histopathological changes in the internal organs of both fish are in agreement with those observed by many investigators on aquatic research [20][21][22][23][24] who have studied the effects of different pollutants on fish organs in such aquatic environment [25].…”

Section: Histological Alterationssupporting

confidence: 89%

Histopathological biomarking changes in the internal organs of Tilapia (Oreochromis niloticus) and catfish (Clarias gariepinus) exposed to heavy metals contamination from Dandaru pond, Ibadan, Nigeria

Abiona

Anifowose

Awojide

et al. 2019

Journal of Taibah University for Science

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The study assessed histopathological alterations in the organs of Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (Catfish) harvested from Dandaru Pond, Ibadan, Nigeria. The histopathological effects were evaluated through biomarkings and heavy metals measurements of the fish organs and tissue. Results showed the kidney with sign of coagulated necrosis of the renal tubules. In the liver, there were hepatocytes which appeared foamy and contained large-sized vacuoles. The gills appeared with some congestion in their capillaries in addition to numerous bluish staining walled-off structures. There was severe thinning of the myocardial fibres in the fish heart. The chambers were moderately congested. The fish organs contained 0.06-0.90 µg/g lead. The Pb concentrations and those of manganese and iron exceeded the WHO permissible limit in fish. It is conclusive that the bioaccumulated heavy metals in the tissue and fish organs consequently impaired their histopathological condition, and thus affect fish health status.

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“…In accordance with earlier literature, the gills of the sh with high bioaccumulation suffered severe lamellar fusion, hyperplasia, hypertrophy and epithelial liing, swelling and deformed lamella, in some parts sloughing off and curving of lamellae. [72][73][74] Their liver hepatocytes and bile ducts showed necrosis of parenchyma, vacuolation, congestion of blood vessels, pyknosis and inltration of leucocytes as observed earlier by Zheng et al, 2011 and Fatima and Usmani, 2013. 75,76 Dilated kidney tubules and vacuolated glomeruli with no Bowman's space, degeneration of glomeruli and necrosis of hematopoietic tissue were observed as in previous reports made by Perera et al, 2016; Kaur and Dua 2014.…”

Section: Histopathologysupporting

confidence: 52%

A study on the impact of water quality on the murrel fishChanna striataandChanna punctatafrom three major Southern Tamilnadu rivers, India

2018

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Studies on biomarkers of oxidative stress and associated genotoxicity and histopathology in Channa punctatus from heavy metal polluted canal

Cited by 111 publications

References 44 publications

Chelator in Environmentally-exposed Poor Detoxifiers: An Adjuvant Hepatoprotective Treatment for Poly-drug Users?

Chelator in Environmentally-exposed Poor Detoxifiers: An Adjuvant Hepatoprotective Treatment for Poly-drug Users?

Histopathological biomarking changes in the internal organs of Tilapia (Oreochromis niloticus) and catfish (Clarias gariepinus) exposed to heavy metals contamination from Dandaru pond, Ibadan, Nigeria

A study on the impact of water quality on the murrel fishChanna striataandChanna punctatafrom three major Southern Tamilnadu rivers, India

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