Studies on buffalo babesiosis in Hubei Province, China

Liu, Zhongling; Jun, Zhao; Ma, Lei; Yao, Bao-An

doi:10.1007/bf02632914

Cited by 22 publications

(11 citation statements)

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“…In contrast, bovine cattle, exotic in the Americas, has only recently come in contact with the vector and pathogen and may thus be highly susceptible to tick infestation and pathogen infection. In this context, it is noteworthy mentioning that clinical cases of babesiosis are commonly reported in water buffalos from China and India, which can be lethal in untreated animals 5,6 . Originally it had been thought that buffalo babesiosis is, as bovine babesiosis, caused by B. bovis .…”

Section: Resultsmentioning

confidence: 99%

“…However, recent evidence demonstrates that the causative agent of buffalo babesiosis is B. orientalis. 5,7 B. orientalis can be distinguished from B. bovis by its smaller size and different morphology and is transmitted by Rhipicephalus haemaphysaloides. 5 Although R. haemaphysaloides feeds on cattle, it has been shown that parasitemia of B. orientalis does not develop and that it is nonpathogenic for bovines 8 .…”

Section: Resultsmentioning

confidence: 99%

“… 5,7 B. orientalis can be distinguished from B. bovis by its smaller size and different morphology and is transmitted by Rhipicephalus haemaphysaloides. 5 Although R. haemaphysaloides feeds on cattle, it has been shown that parasitemia of B. orientalis does not develop and that it is nonpathogenic for bovines 8 . In Argentina and in other countries of the neotropics climatic conditions might, in principle, allow the R. haemaphysaloides vector to thrive, but it has so far not been detected in this continent.…”

Section: Resultsmentioning

confidence: 99%

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Water Buffalos as Carriers of Babesia bovis in Argentina

Ferreri¹,

Benítez

Dominguez³

et al. 2008

Annals of the New York Academy of Sciences

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The tick-transmitted hemoprotozoan Babesia bovis is a major causative agent of bovine babesiosis, an often fatal disease of cattle. The disease is widespread in the northeastern region of Argentina, where an increasing part of the livestock is composed of water buffalos. Although clinical cases of buffalo babesiosis have not been reported so far, the pathogen-transmitting tick vector has been occasionally observed by us to be feeding on water buffalos. We therefore set out to examine whether buffalos may constitute a reservoir of the parasite. Competitive enzyme-linked immunosorbent assay (cELISA) detected B. bovis-specific antibodies in 20% of investigated buffalos (21/103), while direct detection of the pathogen by nested PCR was demonstrated in 34% of the animals (35/103). In one field, more than 60% of investigated animals (22/36) tested positive by nested PCR. These results are discussed in the context of buffalo babesiosis reported in other countries and in view of the currently effected control measures against bovine babesiosis in the region.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Water Buffalos as Carriers of Babesia bovis in Argentina

Ferreri¹,

Benítez

Dominguez³

et al. 2008

Annals of the New York Academy of Sciences

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“…It has been reported that several parasites can cause buffalo babesiosis, including Babesia bovis , Babesia bigemina and Babesia orientalis [ 4 , 5 ]. Babesia orientalis was identified to be transmitted by Rhipicephalus haemaphysaloides in its original description in 1997 [ 6 , 7 ]. Clinical symptoms for B. orientalis infection include anemia, fever, icterus and hemoglobinuria, and it can cause death in serious cases [ 3 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes

Guo

Sun

et al. 2018

Parasites Vectors

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BackgroundThe spherical body, a membrane bound organelle localized in the apical organelle complex, is unique to Babesia and Theileria spp. The spherical body proteins (SBPs) secreted by spherical bodies include SBP1, SBP2, SBP3 and SBP4. Up to now, only SBP3 has been characterized in Babesia orientalis.MethodsThe BoSBP4 gene was amplified from cDNA and gDNA and cloned into the pGEX-6P-1 vector by homologous recombination, sequenced and analyzed by bioinformatics tools. The amino acid (aa) sequence of BoSBP4 was compared with that of Babesia bovis and Babesia bigemina as well as SBP3 of B. orientalis. The immunoreactivity was evaluated by incubating recombinant BoSBP4 (rBoSBP4) with the serum of B. orientalis-infected water buffalo. The native form of BoSBP4 was identified by incubating lysate of B. orientalis-infected water buffalo erythrocytes with the anti-rBoSBP4 mouse serum. The cellular localization of BoSBP4 was determined by indirect immunofluorescence assay.ResultsThe full length of the BoSBP4 gene was estimated to be 945 bp without introns, encoding a 314 aa polypeptide with a predicted molecular weight of 37 kDa. The truncated recombinant protein was expressed from 70 to 945 bp as a GST fusion protein with a practical molecular weight of 70 kDa. BoSBP4 shared a 40% and 30% identity with B. bovis and B. bigemina, respectively. Furthermore, it was 31% identical to SBP3 of B. orientalis. BoSBP4 was identified in the lysate of B. orientalis-infected water buffalo erythrocytes with a molecular weight of 37 kDa, corresponding to the expected molecular mass of BoSBP4. The result of rBoSBP4 with positive serum revealed that BoSBP4 can elicit an immune response to B. orientalis-infected water buffalo. The cellular localization of BoSBP4 was detected to be adjacent to the merozoite nucleus in the intracellular phase, followed by the diffusion of the fluorescence of BoSBP4 into the cytoplasm of B. orientalis-infected erythrocytes as puncta-like specks and a gradual increase of the fluorescence.ConclusionsIn this study, SBP4 in B. orientalis was characterized for the first time. It may play a key role in interaction with the host cell by being secreted into the cytoplasm of the B. orientalis-infected erythrocytes to facilitate parasite growth and reproduction.

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“…em búfalos (Liu et al 1997, Terkawi et al 2011, estes estudos ainda são raros quando se refere a Anaplasma marginale. No Brasil, Corrêa (2011) observou que a soroprevalência de A. marginale em bubalinos é bem distinta dos valores observados para bovinos.…”

Section: Discussionunclassified

Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará

et al. 2014

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Pesq. Vet. Bras. 34(1):11-14, janeiro 2014 11 RESUMO.-O objetivo do estudo foi testar a prevalência sorológica e molecular de Anaplasma marginale em búfalos do municipio de Soure, Ilha de Marajó, estado do Pará, Brasil. Para a pesquisa sorologica foram selecionados randomicamente 800 animais e para a pesquisa molecular 50 destes animais foram aleatoriamente escolhidos. Para quantificar a prevalência sorológica utilizou-se o ensaio de imunoadsorção enzimático indireto (iELISA) com antígeno total contendo proteínas de superfície externa e para quantificar a prevalência molecular utilizou-se a reação em cadeia da polimerase (PCR), envolvendo a amplificação de fragmento gênico da proteína de superfície maior 5 (MSP5). A prevalência de animais positivos no ELISA para A. marginale foi de 25% (200/800). Na PCR foi detectada a presença de A. marginale em 2% (1/50) dos animais. Embora apenas um animal tenha sido positivo na PCR, observou-se que o mesmo foi negativo no ELISA. A presença do agente, mesmo em baixa prevalência, mostra que os bubalinos podem funcionar como um importante reservatório desse patógeno para os rebanhos bovinos da região norte do Brasil.

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Studies on buffalo babesiosis in Hubei Province, China

Cited by 22 publications

References 0 publications

Water Buffalos as Carriers of Babesia bovis in Argentina

Water Buffalos as Carriers of Babesia bovis in Argentina

Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes

Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará

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