Studies on effectiveness of PTT on 3D tumor model under microfluidic conditions using aptamer-modified nanoshells

Kalinowska, Dominika; Grabowska-Jadach, Ilona; Liwinska, Monika; Drozd, Marcin; Pietrzak, Mariusz; Dybko, Artur; Brzózka, Zbigniew

doi:10.1016/j.bios.2018.10.069

Cited by 36 publications

(16 citation statements)

References 40 publications

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“…(20) The development of these microfluidic systems to mimic tumors are on use in a number of therapies, raising the interest of scientific community, in order to replace the use of murine models. (21)(22)(23) One of these therapies is applied by hyperthermia treatments, such as MHT in tumors.…”

Section: ❚ Discussionmentioning

confidence: 99%

Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model

Mamani

Marinho

Rego

et al. 2019

Einstein (São Paulo)

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Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the magnetic nanoparticles at 300 Gauss and 305kHz. A preculture of C6 cells was performed before the 3D cells culture on the chip. The process of magnetic hyperthermia on the Chip was performed after administration of 20µL of magnetic nanoparticles (10mgFe/mL) using the parameters that generated the specific absorption rate value. The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining: calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes. Results: Magnetic nanoparticles when submitted to the alternating magnetic field (300 Gauss and 305kHz) produced a mean value of the specific absorption rate of 115.4±6.0W/g. The 3D culture of C6 cells evaluated by light field microscopy imaging showed the proliferation and morphology of the cells prior to the application of magnetic hyperthermia therapy. Fluorescence images showed decreased viability of cultured cells in organ-on-a-Chip by 20% and 100% after 10 and 30 minutes of the magnetic hyperthermia therapy application respectively. Conclusion: The study showed that the therapeutic process of magnetic hyperthermia in the glioblastoma on-achip model was effective to produce the total cell lise after 30 minutes of therapy.

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Section: ❚ Discussionmentioning

confidence: 99%

Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model

Mamani

Marinho

Rego

et al. 2019

Einstein (São Paulo)

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“…A model that can replicate more closely the real environment, can provide more accurate data as demonstrated by Carvalho et al. 92 In this reasoning, several studies were performed taking in consideration this feature to obtain more reliable data concerning therapies assessment, such as photodynamic therapy, 93,94 concerning physiological data, such as the nanoparticles' transport within the tumour environment, 95,96 or concerning the prediction of the effect of therapies during cancer treatment, such as chemotherapy and radiotherapy. 97 Furthermore, the use of microfluidic devices and nanoparticles has been crucial for the study of new strategies and to overcome some issues related with the development of efficient therapies, such as the low targeting efficiency, and related with drug delivery, namely the retention observed during systemic administration (Figure 7).…”

Section: Microfluidic Devices and Nanoparticles For Therapies' Improvmentioning

confidence: 99%

Finding the perfect match between nanoparticles and microfluidics to respond to cancer challenges

Maia

Reis

Oliveira

2020

Nanomedicine: Nanotechnology, Biology and Medicine

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The clinical translation of new cancer theranostic has been delayed by inherent cancer's heterogeneity. Additionally, this delay has been enhanced by the lack of an appropriate in vitro model, capable to produce accurate data. Nanoparticles and microfluidic devices have been used to obtain new and more efficient strategies to tackle cancer challenges. On one hand, nanoparticles-based therapeutics can be modified to target specific cells, and/or molecules, and/or modified with drugs, releasing them over time. On the other hand, microfluidic devices allow the exhibition of physiologically complex systems, incorporation of controlled flow, and control of the chemical environment. Herein, we review the use of nanoparticles and microfluidic devices to address different cancer challenges, such as detection of CTCs and biomarkers, point-of-care devices for early diagnosis and improvement of therapies. The future perspectives of cancer challenges are also addressed herein.

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“…[12,13] Furthermore, microfluidic integration on a large-scale enables highthroughput operation and investigation of cell/tumor samples. [14,15] In the past several decades, research scholars around the globe have developed many microfluidic tumor manipulation systems that depend on passive microwells, [16][17][18][19] microdroplets, [20,21] microhydrogels, [22][23][24] active pneumatic microstructures (PμSs), [25][26][27] as well as 3D acoustic tweezers [28,29] for controllable 3D tumor cultivation. These advances have provided several beneficial properties, such as facile and efficient operation, homogeneous and large-scale tumor production, and throughput monitoring.…”

Section: Introductionmentioning

confidence: 99%

Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device

Liu

Han

et al. 2021

Biotechnology Journal

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Background: Cancer has been responsible for a large number of human deaths in the 21st century. Establishing a controllable, biomimetic, and large-scale analytical platform to investigate the tumor-associated pathophysiological and preclinical events, such as oncogenesis and chemotherapy, is necessary. Methods and Results:This study presents antitumor investigation in a parallel, largescale, and tissue-mimicking manner based on well-constructed chemical gradients and heterotypic three-dimensional (3D) tumor cocultures using a multifunctionintegrated device. The integrated microfluidic device was engineered to produce a controllable and steady chemical gradient by manipulative optimization. Array-like and size-homogeneous production of heterotypic 3D tumor cocultures with in vivolike features, including similar tumor-stromal composition and functional phenotypic gradients of metabolic activity and viability, was successfully established. Furthermore, temporal, parallel, and high-throughput analyses of tumor behaviors in different antitumor stimulations were performed in a device based on the integrated operations involving gradient generation and coculture. Conclusion:This achievement holds great potential for applications in the establishment of multifunctional tumor platforms to perform tissue-biomimetic neoplastic research and therapy assessment in the fields of oncology, bioengineering, and drug discovery.

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Studies on effectiveness of PTT on 3D tumor model under microfluidic conditions using aptamer-modified nanoshells

Cited by 36 publications

References 40 publications

Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model

Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model

Finding the perfect match between nanoparticles and microfluidics to respond to cancer challenges

Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device

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