Studies on Experimental Jernbrana Disease in Bali Cattle. 11. Clinical Signs and Haematological Changes

Soesanto, M.; Soeharsono, S.; Budiantono, A.; Sulistyana, K.; Tenaya, M.; Wilcox, G. E.

doi:10.1016/s0021-9975(08)80135-1

Cited by 41 publications

(37 citation statements)

References 6 publications

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“…Jembrana disease constitutes the main concern in Bali cattle industry due to the high mortality of the infected cattle. In experimentally infected Bali cattle, the mortality rate was about 17 % [20]. This was consistent with data obtained from field observations [4].…”

Section: Introductionsupporting

confidence: 90%

“…Distinguishing BIV-infection was only made feasible by using a BIVspecific monoclonal antibody that only recognizes the unique BIV GAG epitope, which is not shared by JDV [14]. During the acute phase, high titre of infectious JDV viral particles is found in plasma [18,20]. Viruses are also abundantly present in secreted fluids, namely milk and saliva.…”

Section: Discussionmentioning

confidence: 99%

“…This is in contrast to the chronic and progressive diseases over a long incubation periods typically associated with most lentiviral infections [5]. During the febrile stage of the disease, a high titre of viral particles is found in the plasma fraction of the blood [18,20]. Viruses are also detected in secreted fluids, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

et al. 2015

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Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. An easy and rapid diagnostic method is essential for further control this disease. We used a reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with lateral flow dipstick (LFD), based on conserved tm subunit of Jembrana disease virus env gene. The RT-LAMP conditions were optimized by varying the concentration of MgSO 4 , betaine, dNTP, and temperature as well as the time and duration of reaction. The primers sensitivity for JDV was confirmed. The method was able to detect env-tm gene dilution which contained 2 9 10 -15 g of template. Comparatively, the sensitivity of RT-LAMP/LFD was 100-fold more sensitive than reverse transcription-polymerase chain reaction. The primers specificity for JDV was also confirmed using positive and negative controls. This work also showed that virus detection could be done not only on total RNA extracted from blood but various organs could also be analyzed for the presence of JDV using RT-LAMP/LFD method. The whole process, including the LAMP reaction and the LFD hybridization step only lasts approximately 75 min. Results of analysis can be easily observed with naked eyes without addition of any chemical or further analysis. The combination of RT-LAMP with LFD makes the method a more suitable diagnostic tool in conditions where sophisticated and expensive equipments are not available for field investigations on Jembrana disease in Bali cattle.

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Section: Introductionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

et al. 2015

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“…These cattle were maintained in screened animal rooms and were all susceptible to experimental infection [1,6].…”

Section: Bali Cattlementioning

confidence: 99%

“…Bos sondaicus) in Indonesia with a case fatality rate of about 20%. The incubation period is short (5-12 days) [1]. The principal lesions are an intense non-follicular proliferation of lymphoblastoid cells in lymphoid organs, and a lymphoid infiltrate in other visceral organs [2].…”

Section: Introductionmentioning

confidence: 99%

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Soeharsono¹,

Wilcox

Putra³

et al. 1995

Epidemiol. Infect.

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SummaryMethods of transmission of Jembrana disease, an acute and severe disease of Bali cattle (Bos javanicus) caused by a recently-identified bovine lentivirus known as Jembrana disease virus, are described. During the acute disease virus can be detected in saliva and milk. There is evidence of direct transmission from acutely affected animals in close contact with susceptible cattle, possibly by virus in these secretions infecting cattle by the conjunctiva!, intranasal or oral routes, by which it was possible to infect cattle experimentally. During the acute disease the titre of infectious virus in blood is high, about 108 50% cattle infectious units (ID50)/ml, and it is probable that the virus is also transmitted mechanically by haematophagous arthropods. Recovered cattle are also a potential but probably infrequent source of infection; recovered cattle are persistently viraemic but the titre of infectious virus in blood decreases to about 101 ID50/ml by 60 days after recovery from the acute disease, and virus cannot be detected in secretions.

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Tat protein expression in MDBK cells does not confer susceptibility to bovine immunodeficiency virus

Kempster

Collins

Brownlie

2002

Archives of Virology

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The ability of BIV strain R29 to infect bovine cell lines in the presence or absence of a functional lentiviral Tat protein is described. Jembrana disease virus (JDV) Tat protein was stably expressed in MDBK cells. No viral replication could be detected in this cell line after infection with BIV R29. Transfection of MDBK cells and MDBK Tat expressing cells with BIV R29 proviral DNA established that BIV R29 could not replicate in MDBK cells. Whether viral entry into MDBK cells is also a block to BIV R29 infection of MDBK cells has yet to be established.

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Studies on Experimental Jernbrana Disease in Bali Cattle. 11. Clinical Signs and Haematological Changes

Cited by 41 publications

References 6 publications

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus

The transmission of Jembrana disease, a lentivirus disease of Bos javanicus cattle

Tat protein expression in MDBK cells does not confer susceptibility to bovine immunodeficiency virus

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