Abstract. In this paper, storage effect on biodiesel properties for high blending ratio and the emission are evaluated. The blend level of biodiesel is denoted as B, followed by the percentages of pure biodiesel. The samples used are conventional diesel (STD), B80, B90 and B100 were stored at ambient temperature (24 ˚C) for nine week. The properties of biodiesel which are acid value, flash point, viscosity, density and water content were measured according to ASTM D6751 biodiesel fuel standard. Results show there are no significant effects on it properties while stored in long duration at ambient temperature. Meanwhile, increasing biodiesel ratio is found to enhance the combustion process, resulting in decreased the HC for emissions.