STUDIES ON THE INTRACELLULAR LOCALIZATION OF ANTERIOR PITUITARY HORMONES BY MEANS OF DIFFERENTIAL CENTRIFUGATION<sup>1</sup>

Brown, Jennifer; LaBella, Frank S.; Ulvedal, F

doi:10.1210/endo-66-1-1

Cited by 11 publications

(3 citation statements)

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“…The S~ExSP contained approximately one-third and the S2D about one-fifth of the TSH activity found in the WH. Brown et al (19) have reported that TSH is associated with the mitochondria. This observation is not supported since the active S2EISP containing TSH has been assayed for the presence of succinic dehydrogenase and none was found.…”

Section: T H Y R O T R O P I G H O R M O N E ( T S H )mentioning

confidence: 99%

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Isolation and Biochemical Study of Secretory Granules From Rat Pituitary Glands

Perdue

McShan

1962

The Journal of Cell Biology

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A B S T R A C TSecretory granules from anterior pituitary glands of young adult male castrate rats were isolated by differential centrifugation, microfiltration, and discontinuous density gradient centrifugation. The granules wcre obtained as pellets, sectioned, and studicd with the electron microscope. A major part of the gonadotropin and a substantial amount of the TSH were associated with the isolated granules. Negligiblc amounts of growth hormone and prolactin were present as contaminants. Succinic dehydrogenasc, glucosc-6-phosphatase, acid protease, and acid and alkaline phosphatascs were not found in the granules. Alkaline proteasc was the only enzyme found to be associated with the granules, and it is suggested, in the light of these results, that the alkaline protease may be involved in the release of the hormones. I N T R O D U C T I O NElectron microscope studies of anterior pituitary glands from rats by Farquhar and co-workers (1-5) have shown that the two representative cell types, acidophils and basophils, contain secretory granules of a specific maximum diameter. Certain of the acidophilic cells contain 350-m# and others 600-m# secretory granules reported to be associated, respectively, with growth and lactogenic hormones, while the two basophilic cell types have granules 140 m# (thyrotropin) and 200 m/.t (gonadotropins) in diameter (6).The separation of pituitary cytoplasmic particulates into various fractions and the determination of the hormones associated with them have been the objective of several workers employing differential centrifugation procedures similar to those used by Hogeboom et al. (7) for the preparation of liver particulates. These pituitary studies (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were done with glands from different species, using different homogenizing media, and different times and speeds of centrifugation. The results of these studies are of interest in that they provided information concerning the hormonal activities of the fractions. The degree of purity of these fractions was not established and, as a consequence, generalizations cannot be made as to the specific particulates with which the hormonal activities are associated. In a recent study, however, Hartley et al. (6,21), using differential centrifugation, microfiltration, and isopycnic gradient centrifugation succeeded in isolating highly purified granules, 200 m# in diameter, from anterior pituitary glands of castrate rats. A substantial amount of the original homogenate's gonadotropic hormone activity was associated with these granules.The present report is concerned with the extension of this work, in that a shorter procedure was developed for obtaining the granules, young 159 on May 12, 2018 jcb.rupress.org Downloaded from

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Section: T H Y R O T R O P I G H O R M O N E ( T S H )mentioning

confidence: 99%

“…These pituitary studies (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were done with glands from different species, using different homogenizing media, and different times and speeds of centrifugation. The results of these studies are of interest in that they provided information concerning the hormonal activities of the fractions.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Biochemical Study of Secretory Granules From Rat Pituitary Glands

Perdue

McShan

1962

The Journal of Cell Biology

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“…Among the methods for demonstrating the cellular sites of production of anterior pituitary hormones are special histochemical techniques correlated with clinical and experimental findings (1)(2)(3), ultracentrifugation studies (4,5), tissue culture methods (6), and fluorescent antibody methods (7)(8)(9). The fluorescent antibody technique has the distinct advantages of specificity and of being applicable to pituitaries in histologic section.…”

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confidence: 99%