Studies on Tryptophan Pyrrolase in Drosophila Melanogaster

Kaufman, Seymour

doi:10.1093/genetics/47.7.807

Cited by 53 publications

(10 citation statements)

References 4 publications

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“…Suppressible vermilion ( V I ) flies have less than 1 % of the wild-type activity and may not possess any activity at all. These results differ somewhat from the findings of both BAGLIONI (1960) and KAUFMAN ( 1962). BAGLIONI found 30% of wild-type activity in suppressedvermilion flies and 15% in us flies, while KAUFMAN found 15% and 1 to 2%, respectively.…”

Section: Discussioncontrasting

confidence: 90%

“…A Lineweaver-Burk plot of the data is shown in Figure 1B. The Michaelis-Menten constant (K,) for wild-type and for su-U u1 enzyme from these data is approximately 1 x M, in agreement with an earlier report (KAUFMAN 1962) for crude enzyme preparations from wild-type and suppressed-vermilion flies for many hours. Partially purified tryptophan pyrrolase from wild-type flies has a much higher activity than crude preparations, and the reaction rate begins to decrease after 2 or 3 hours (Figure ID).…”

Section: Kineticssupporting

confidence: 89%

“…Flies were raised and crude extracts and partially purified tryptophan pyrrolase were prepared, as described elsewhere ( MARZLUF, in preparation). Enzymatic assays were conducted by use of the Bratton-Marshall test for kynurenine (KAUFMAN 1962).…”

Section: Materials a N D M E T H O D Smentioning

confidence: 99%

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ENZYMATIC STUDIES WITH THE SUPPRESSOR OF VERMILION OF DROSOPHILA MELANOGASTER

Marzluf¹

1965

Genetics

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Section: Discussioncontrasting

confidence: 90%

Section: Kineticssupporting

confidence: 89%

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ENZYMATIC STUDIES WITH THE SUPPRESSOR OF VERMILION OF DROSOPHILA MELANOGASTER

Marzluf¹

1965

Genetics

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“…Two of the enzymes in this pathway. tryptophan pyrrolase (BAGLIONI 1959(BAGLIONI , 1960KAUFMAN 1962; MARZ-LUF 1965a, b) and kynurenine formamidase (GLASSMAN 1956) have been described. and the absence of tryptophan pyrrolase in the vermilion mutant of Drosophila has been documented.…”

mentioning

confidence: 99%

“…Endogenous 3-hydroxykynurenine was assayed by the same method in homogenized, deproteinized, tissue samples. Preparation and Q S S ~ of tryptophan pyrrolase: Preparation and assay of this enzyme were carried out using the method of KAUFMAN (1962) as modified by MARZLUF (1965). Estimations of endogenous diazotizable amine were made using the Bratton-Marshall procedure, as modified by BAGLIONI (1959), on extracts without incubation.…”

mentioning

confidence: 99%

ENZYMATIC STUDIES ON THE HYDROXYLATION OF KYNURENINE IN DROSOPHILA MELANOGASTER

Ghosh¹,

Forrest²

1967

Genetics

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HE classic pathway for brown pigment synthesis in Drosophila melanogaster,as determined by the accumulation of intermediates, involves the sequence, tryptophan -+ formyl kynurenine + kynurenine -+ 3-hydroxykynurenine -+ brown pigments (for review see ZIEGLER 1961). Two of the enzymes in this pathway. tryptophan pyrrolase (BAGLIONI 1959(BAGLIONI , 1960KAUFMAN 1962; MARZ-LUF 1965a, b) and kynurenine formamidase (GLASSMAN 1956) have been described. and the absence of tryptophan pyrrolase in the vermilion mutant of Drosophila has been documented. However. the enzyme catalyzing the conversion of kynurenine into hydroxykynurenine has never been described in Drosophila, although it has been shown to be present in a mammalian system (DE CASTRO, PRICE and BROWN 1956), and some of its characteristics, as studied in this system, are known (SAITO, HAYAISHI. and ROTHBERG 1957). In this paper the existence of this enzyme in Drosophila, its virtual absence in the mutant cinnabar, and its very greatly reduced activity in the mutant white, are demonstrated. On the other hand, tryptophan pyrrolase activity in extracts of the cinnabar and white mutants is the samc as in extracts ,of wild type. MATERIALS A N D METHODSWild type Oregon-R and the white mutant of Drosophila melanogaster were from stocks malntained in our laboratories. The cinnabar mutant was obtained from the California Institute of Technology. Flies were raised at a temperature of 23" in large populations accordhg to the method of MITCHELL and MITCHELL (1964). Eggs were collected over 4 to 5 hours in plastic bread boxes spread with standard cornmeal agar medium. Samples of larvae and pupae, at different stages of development, were harvested for experiments following the method of MITCHELL and MITCHELL. Preparation. partial purd;fication and assay of kynurenine hydroxylase in wild-type. (A) Crude homogenate:To each gram of chilled material (larvae, pupae or adults) were added 1.4 amoles NADPH?. 2 0 units glucose-6-phosphate dehydrogenase (Sigma) (KORNBERG 1950), 130 amoles glucose-6-phosphate, 1.2 pmoles potassium cyanide, and 260 pmoles phosphate buffer (pH 7.5). Total volume of fluid added was 1.4 ml. A glass-Teflon tissue grinder fitted into a mechanical

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