Study of acetylcholinesterase inhibitors using CE with contactless conductivity detection

This review provides systematic coverage of examples in the field of in-capillary electrophorecially mediated microanalysis (EMMA). The recent developments and applications in the time period up to mid 2011 have been described, as well as relevant older papers. The basic principles and modes of in-capillary assays have been demonstrated. An overview is also given of the various injection, separation and detection modes implemented in combination with EMMA. The review is presented in two parts mainly dealing with (i) enzymatic and (ii) derivatization or chemical reactions. Finally, the future trends of CE in performing and monitoring reactions have been drawn.

show abstract

“… Dual electropherograms for dialyzed urine sample assayed for LAP 66. Sample was incubated for 10 min.…”

Section: In‐capillary Assaymentioning

confidence: 99%

“…The feasibility of monitoring the peptic and tryptic digestion of peptides and proteins with EMMA‐C 4 D has been demonstrated by Schuchert‐Shi and Hauser 65. Recently, this group utilized C 4 D to study acetylcholinesterase inhibitors on‐line 66.…”

Section: In‐capillary Assaymentioning

confidence: 99%

Recent developments and applications of EMMA in enzymatic and derivatization reactions

2011

View full text Add to dashboard Cite

show abstract

“…Importantly, synthetic inhibitors are used as medicines to treat neurodegenerative disorders such as Alzheimer's disease (AD). 19 Pharmacological treatment of AD is currently based on AChE inhibitors, such as tacrine, donepezil, rivastigmine, and galantamine, which maintain high levels of ACh at the muscarinic and nicotinic receptors in the central nervous system, exerting beneficial effects on cognitive, functional, and behavioral symptoms of the disease. 20 Therefore, AChE inhibition assay is utilized to screen for potent AChE inhibitors from edible resources such as black soybean seed coat.…”

Section: Discussionmentioning

confidence: 99%

Black Soybean Extract Protects Against TMT-Induced Cognitive Defects in Mice

Jeong

Kim

et al. 2014

Journal of Medicinal Food

View full text Add to dashboard Cite

To find a neuroactive compound with a potent inhibitory effect on acetylcholinesterase (AChE) and in vivo anti-amnesic activity from natural resources, we evaluated anthocyanins and nonanthocyanins from black soybean extract. Nonanthocyanins from black soybean extract were the most potent and dose-dependent AChE inhibitors. Intracellular reactive oxygen species accumulation resulting from H2O2 treatment was significantly decreased compared with cells treated with H2O2 only. Nonanthocyanins were also neuroprotective against H2O2 treated neurotoxicity by 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay. Finally, nonanthocyanins from black soybean in the preadministration group attenuated trimethyltin (TMT)-induced memory injury in both in vivo tests. AChE, prepared from mice brain tissues, was inhibited by nonanthocyanins from black soybean in a dose-dependent manner. Malondialdehyde generation in the brain homogenates of mice treated with nonanthocyanins from black soybean was decreased. We concluded that nonanthocyanins from black soybean had an efficacious in vitro AChE inhibitory activity, and protected against H2O2-induced neurotoxicity. In addition, our findings suggest that nonanthocyanins from black soybean may improve the TMT-induced learning and memory deficit because of AChE inhibition of mice brain tissue. Consequently, these results demonstrate that the nonanthocyanins from black soybean could possess a wide range of beneficial activities for neurodegenerative disorders.KEY WORDS: acetylcholinesterase black soybean cognitive function Glycine max (L.) Merr. syringic acid trimethyltin INTRODUCION Soybean (Glycine max) has been one of the long important nutritional sources since ancient times. Soybeans are extremely versatile and can be made into a variety of foods. Asians including Koreans consume an average of 20 to 80 g of traditional soy foods daily, the most common of which include tofu, miso, and tempeh. Americans consume much less soy, only about 1 to 3 g daily, and this is mostly in processed forms such as soy drinks, breakfast cereals, energy bars, and soy burgers.

show abstract

“…However, the sensitivity of CE is a shortcoming because of the short light path. Hence, a variety of detection instruments are combined directly with CE, including laser‐induced fluorescence, ultraviolet absorbance, MS, and electrochemical detectors . In spite of all its assets, CE–Q‐TOF–MS remains rarely used in the determination of natural products.…”

Section: Introductionmentioning

confidence: 99%

Solid acids assisted matrix solid‐phase dispersion microextraction of alkaloids by capillary electrophoresis coupled with quadrupole time‐of‐flight mass spectrometry

Wang

Dong

Yang

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

The quantification of three alkaloids is important because quantitative study is a means of assessing the reliability of the experimental method, and three alkaloids of peimine, peiminine, and peimisine are main active ingredients in Chinese Pharmacopoeia 2015. An effective method based on the matrix solid-phase dispersion microextraction was developed for the extraction of alkaloid compounds in Fritillariae Thunbergii Bulbus. Target analytes were analyzed by capillary electrophoresis coupled with quadrupole time-of-flight mass spectrometry. The optimized experimental condition was that 50 mg Fritillariae Thunbergii Bulbus was blended homogeneously with 10 mg citric acid for 5 min. Two hundred microliters of water acidized by 1 mol/L hydrochloric acid (pH = 4.5) was selected to elute tested alkaloids. The results demonstrated that the investigated method had low limits of detection (1.32-1.59 ng/mL), good recoveries (86.63-98.12%), and reproducibility (relative standard deviations of peak areas < 0.87%). The proposed matrix solid-phase dispersion microextraction coupled with capillary electrophoresis combined with quadrupole time-of-flight mass spectrometry was successfully applied for the extraction of alkaloids in plants. K E Y W O R D Scapillary electrophoresis, isosteroidal alkaloids, matrix solid-phase dispersion microextraction, quadrupole time-of-flight mass spectrometry, traditional Chinese medicine

show abstract

Study of acetylcholinesterase inhibitors using CE with contactless conductivity detection

Cited by 18 publications

References 36 publications

Recent developments and applications of EMMA in enzymatic and derivatization reactions

Recent developments and applications of EMMA in enzymatic and derivatization reactions

Black Soybean Extract Protects Against TMT-Induced Cognitive Defects in Mice

Solid acids assisted matrix solid‐phase dispersion microextraction of alkaloids by capillary electrophoresis coupled with quadrupole time‐of‐flight mass spectrometry

Contact Info

Product

Resources

About