Study of DNA integrity in alpha-particle radiobiological experiments using thin CR-39 detectors

Chan, Ka Fai; Yum, E.H.W.; Wan, Chi-Keung; Fong, Wang‐Fun; Yu, K.N.

doi:10.1016/j.radmeas.2008.04.043

Cited by 12 publications

(5 citation statements)

References 9 publications

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“…BIOSTACK experiment [2,3] and cell-coated CR39 detectors [4]) were hindered by a limited set of physical parameters that could be obtained. In addition, inability to autoclave the detector before cell deposition hindered the sterile cell cover.…”

Section: Introductionmentioning

confidence: 99%

Engineering cell-fluorescent ion track hybrid detectors

et al. 2013

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BackgroundThe lack of sensitive biocompatible particle track detectors has so far limited parallel detection of physical energy deposition and biological response. Fluorescent nuclear track detectors (FNTDs) based on Al2O3:C,Mg single crystals combined with confocal laser scanning microscopy (CLSM) provide 3D information on ion tracks with a resolution limited by light diffraction. Here we report the development of next generation cell-fluorescent ion track hybrid detectors (Cell-Fit-HD).MethodsThe biocompatibility of FNTDs was tested using six different cell lines, i.e. human non-small cell lung carcinoma (A549), glioblastoma (U87), androgen independent prostate cancer (PC3), epidermoid cancer (A431) and murine (VmDk) glioma SMA-560. To evaluate cell adherence, viability and conformal coverage of the crystals different seeding densities and alternative coating with extracellular matrix (fibronectin) was tested. Carbon irradiation was performed in Bragg peak (initial 270.55 MeV u−1). A series of cell compartment specific fluorescence stains including nuclear (HOECHST), membrane (Glut-1), cytoplasm (Calcein AM, CM-DiI) were tested on Cell-Fit-HDs and a single CLSM was employed to co-detect the physical (crystal) as well as the biological (cell layer) information.ResultsThe FNTD provides a biocompatible surface. Among the cells tested, A549 cells formed the most uniform, viable, tightly packed epithelial like monolayer. The ion track information was not compromised in Cell-Fit-HD as compared to the FNTD alone. Neither cell coating and culturing, nor additional staining procedures affected the properties of the FNTD surface to detect ion tracks. Standard immunofluorescence and live staining procedures could be employed to co-register cell biology and ion track information.ConclusionsThe Cell-Fit-Hybrid Detector system is a promising platform for a multitude of studies linking biological response to energy deposition at high level of optical microscopy resolution.

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Section: Introductionmentioning

confidence: 99%

Engineering cell-fluorescent ion track hybrid detectors

et al. 2013

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“…Dose can then be calculated from the beam area and LET spectrum. Measuring fluence can be done using CR39 track etch film [12], though this only provides a retroactive quantification, or using an in-line ion chamber or other beam monitor. We have used this approach historically for our broad beam irradiation platform and continue to use it for microbeam irradiations.…”

Section: Alternatives To Absolute Dosementioning

confidence: 99%

Traceable dosimetry for MeV ion beams

2022

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Standard dosimetry protocols exist for highly penetrating photon and particle beams used in the clinic and in research. However, these protocols cannot be directly applied to shallow penetration MeV-range ion beams. The Radiological Research Accelerator Facility has been using such beams for almost 50 years to irradiate cell monolayers, using self-developed dosimetry, based on tissue equivalent ionization chambers. To better align with the internationally accepted standards, we describe implementation of a commercial, NIST-traceable, air-filled ionization chamber for measurement of absorbed dose to water from low energy ions, using radiation quality correction factors calculated using TRS-398 recommendations. The reported dose does not depend on the ionization density in the range of 10–150 keV/μm.

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“…Microbeams and CR-39 detectors have been used to study the effect of individual ion hits on single-cell fate ( Chan et al., 2008 ; Friedland et al., 2019 ). Microbeam experiments are not yet capable of fully elucidating the response of cells in a clinical ion beam field due to the field’s complex energy spectrum.…”

Section: Introductionmentioning

confidence: 99%