Study of ion chromatographic behavior of inorganic and organic antimony species by using inductively coupled plasma mass spectrometric (ICP-MS) detection

Ulrich, Nina

doi:10.1007/s002160050810

Cited by 20 publications

(5 citation statements)

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“…First, total intracellular antimony content was measured with FI-ICP-MS (25,26), and the results are summarized under "Total [Sb] i " in Tables 1 and 2. The second method used to determine the content of antimony hydride (SbH 3 ) was FI-HG-ICP-MS (27,28).…”

Section: Resultsmentioning

confidence: 99%

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Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Shaked‐Mishan¹,

Ulrich²,

Ephros³

et al. 2001

Journal of Biological Chemistry

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208

158

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The standard treatment of human visceral leishmaniasis involves the use of pentavalent antimony (Sb V ). Its mechanism of action is unknown because of the limited information available about intracellular antimony metabolism and about the genes that regulate these processes. Herein, flow injection-inductively coupled plasma mass spectrometry (ICP-MS), flow injection hydride generation ICP-MS, and ion chromatography ICP-MS were used to measure antimony accumulation and intracellular metabolism in the human protozoan parasite Leishmania donovani. V reduction has yet to be identified, and it may or may not be enzymatic. This is the first description of intracellular Sb V reducing activity in Leishmania as well as in any prokaryotic or eukaryotic cell.Leishmania donovani is the major causative agent of visceral leishmaniasis. In nature, the parasite exists either as an extracellular promastigote found in the alimentary tract of sandflies or as an obligatory intracellular amastigote found in phagolysosomes of mammalian macrophages(1-3). During the last several years, a number of laboratories have utilized axenic culture of L. donovani amastigotes for the direct evaluation of cell biological and biochemical processes in the amastigote, devoid of the host macrophage (4 -7,8,9,10). This technique has also been used to investigate the mechanism of drug action and resistance as well as for screening of potential new drugs(11-13). The treatment of choice of human visceral leishmaniasis is the administration of pentavalent antimony (Sb V )-containing drugs such as sodium stibogluconate (Pentostam, Wellcome, Beckenham, UK) or meglumine antimoniate (Glucantime, Rhone-Poulenc, Paris, France). Despite extensive use of these compounds over the last decades, the mechanism of action remains unclear.It has been hypothesized that Sb V is not toxic to Leishmania, but rather that it is enzymatically reduced, probably by the host macrophage, to Sb III , the form of antimony that is highly Leishmania-toxic (14 -18). In contrast, it has been shown that Sb V is directly toxic to axenic amastigotes (12,13,19), thus negating the necessity of the macrophage for Sb V reduction. Furthermore, these data imply that either the parasite reduces Sb V to Sb III intracellularly, and thus Sb III is directly toxic to amastigotes, or that both oxidation states of antimony are active against Leishmania amastigotes. The modes of action of the two oxidation states of antimony (Sb III and Sb V ) on Leishmania are, as yet, not fully understood.Several groups have shown obligatory cross-resistance between Sb V , Sb III , and arsenite (As III ) in Leishmania tarentolae, Leishmania major, Leishmania mexicana, L. donovani and Leishmania infantum (11,[20][21][22]. In contrast, it has been suggested that, at least in L. donovani, such cross-resistance does not necessarily occur (12). Furthermore, it has been demonstrated that, when bound to organic compounds, structural similarities exist between Sb III and Sb V (23). For example, the trivalent antimony comp...

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Section: Resultsmentioning

confidence: 99%

“…Each sample contained 100-l aliquots of cell extract, which were diluted 1:100 with water (25,26). For each measurement, aliquots of 10 l were injected with five repetitions.…”

Section: Determination Of Intracellular Antimonymentioning

confidence: 99%

Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Shaked‐Mishan¹,

Ulrich²,

Ephros³

et al. 2001

Journal of Biological Chemistry

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208

158

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“…Therefore, knowledge of the distribution, speciation, and transformation of Sb and As in the soil-plant system of the XKS mine area is important for understanding its geochemical and biological cycling and for evaluating the environmental and human health risks. Despite the significance of quantitative speciation, there is only limited information regarding Sb in the soil-plant system (De Gregori et al, 2007;Dodd et al, 1996;Lintschinger et al, 1997Lintschinger et al, , 1998aLintschinger et al, , 1998bNash et al, 2000;Ulrich, 1998aUlrich, , 1998bUlrich et al, 2000). At present, studies of the XKS mine area have primarily involved the total concentrations of Sb and As, and investigations of the Sb and As species in the environment focused only on the inorganic species, particularly in the soil-plant system.…”

Section: Introductionmentioning

confidence: 99%

Speciation of antimony and arsenic in the soils and plants in an old antimony mine

Wei

Chu

et al. 2015

Environmental and Experimental Botany

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a b s t r a c tThe speciation changes of antimony (Sb) in soil-plant system are largely unknown as compared with those of arsenic (As). In this study, indigenous plants and associated soils were sampled at the Xikuangshan Sb mine (XKS), China. The Sb in the soils (441-1472 mg/kg) were far greater than As (32-354 mg/kg), and the Sb and As availabilities in the soils, were 5.5% and 3.9% in average, respectively. HPLC-ICP-MS revealed the presence of four species of Sb in the soils and plants, including Sb III , Sb V , TMSb and UnkSb (unknown). The use of XANES revealed that the UnkSb consisted of inorganic Sb in the form of Sb V . Inorganic Sb were prevalent in the soil and plant samples at the eight sites, whereas TMSb was observed in only a few of the rhizosphere soils, and, in plants at a few of the sites, primarily in the leaves and to a lesser extent in the stems. Arsenic was detected in the soils primarily as inorganic forms, while, DMA was detected in high proportions in all of the plant tissues at all of the sites. The methylation of Sb was far less than that of As in the indigenous plants at XKS. The results suggest that As and Sb differ in transformation characteristics in the soil-plant system in XKS.

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“…2 For example, Sb(III) compounds like Sb 2 O 3 have been shown to act as inhalative lung carcinogens. 3 Hence the knowledge of the elemental concentration and species distribution of Sb in environmental and biological samples is of great importance.…”

Section: Introductionmentioning

confidence: 99%

“…Many analytical methods have been described for speciation analysis of Sb(III) and Sb(V) such as derivatization by hydride generation [13][14][15][16] and hyphenating HPLC and ICP-MS methods. 3,[17][18][19][20][21] Hydride generation and subsequent analyte detection in the gas phase by different techniques, especially atomic spectrometry, suffer from its susceptibility to interferences from transition metals and hydride-forming elements and the on-line coupling of liquid chromatography with ICP-MS does not necessarily guarantee accurate analytical results. Independent of the other effects also present in elemental speciation analysis, varying compositions in chromatographic peaks influence the ionization efficiency in the plasma and, therefore, hinder exact external calibration curve.…”

Section: Introductionmentioning

confidence: 99%

Antimony speciation in soil samples along two Austrian motorways by HPLC-ID-ICP-MS

et al. 2005

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Distribution of antimony and its inorganic species in soil samples along two traffic routes (A14, Rankweil and S36, Knittelfeld) in Austria was determined, since vehicle emissions are an important anthropogenic source of Sb in soil. The samples were taken along three parallel lines at about 0.2, 2 and 10 m distances from the edge of the road and in two depths range (0-5 and 5-10 cm from the soil surface). The optimized extraction was carried out using 100 mmol L(-1) citric acid at pH 2.08 applying an ultrasonic bath for 45 min at room temperature. Speciation analyses were done using on-line isotope dilution after a chromatographic separation of Sb species. Results of the two traffic routes confirmed significant accumulations of Sb at surface (0-5 cm depth) exceeding the natural background values by more than ten times at the S36 or four times at the A14. Concentrations of the extractable inorganic species decreased to natural background levels within a few meters from the edge of the traffic lane. The predominant Sb species was Sb(V). The Sb(III) concentrations at 5-10 cm depths range are nearly constant with distance from the edges of the two roads. Magnetic susceptibility data of all soil samples show the same distribution pattern as Sb and Sb(V) concentrations along the two traffic roads with an excellent correlation. This is an evidence for an anthropogenic source of Sb such as abrasions of motor vehicles surfaces or braking linings. The input of Sb and its inorganic species at one of the sampling sites (Knittelfeld) in samples taken in 2002 and in those taken recently (2005) was monitored. An increase in Sb (>or=30%), Sb(v)(>or=51%) and Sb(iii)(>or=10%) concentrations was only observed near the edge (

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Study of ion chromatographic behavior of inorganic and organic antimony species by using inductively coupled plasma mass spectrometric (ICP-MS) detection

Cited by 20 publications

References 0 publications

Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani

Speciation of antimony and arsenic in the soils and plants in an old antimony mine

Antimony speciation in soil samples along two Austrian motorways by HPLC-ID-ICP-MS

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