Study of normal and modified nucleosides in serum by RP-HPLC

Abstract

KeyWordsColumn liquid chromatography Nucleosides in serum Tumor markers Factor analysis SummaryModified nucleosides derived predominantly from transfer ribonucleic acid (tRNA) have been studied as possible tumor markers. In this paper a reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been applied to study 15 normal and modified nucleosides in serum. The nucleoside levels in normal human serum were established, and the concentrations of 15 nucleosides in serum from 19 cancer patients were determined. It was found that the concentrations of modified nucleosides were significantly higher in patient sera. Based on 15 nucleoside concentrations in serum, factor analysis could classify correctly 90~ of cancer patients from the normal humans. Furtherwork showed that urine was slightly better than serum when determining nucleosides as biological marker candidates. More work is ongoing to determine the clinical usefulness of modified nucleosides as tumor markers.

Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

“…Nucleosides have been determined in various mammalian milks (Leach et al, 1995;Schlimme et al, 1997;Thorell et al, 1996), in serum (Xu, Enderle, Liebich, & Lu, 2000), in urine by RPC (Vidotto, Fousert, Akkermann, Griesmacher, & Mu¨ller, 2003) and in urine by high-performance liquid chromatography-electrospray ionisation mass spectrometry (Dudley et al, 2004).…”

Excretion pattern investigation of urinary normal and modified nucleosides of breast cancer patients by RP-HPLC and factor analysis method

“…An RP-HPLC method (Liebich et al, 1997a) and a micellar electrokinetic capillary chromatographic (MEKC) method (Liebich et al, 1997bZhao et al, 1998) were developed in our laboratories to separate and quantify 14 nucleosides for MEKC and 16 nucleosides for HPLC in urine. In the meantime, the HPLC method developed has also been used to measure the nucleosides in serum (Xu et al 2000). To test the usefulness of nucleosides as tumor markers, and compare them with the conventional tumor markers, we further apply the HPLC method and factor analysis to investigate the excretion pattern of nucleosides in breast cancer patients.…”

Retention and Separation of Adenosine and Analogues by Affinity Chromatography with an Aptamer Stationary Phase

“…These compounds play an important role in cell biology and biochemistry; therefore, sensitive and accurate detection methods for them are in great demand. High-performance liquid chromatography and capillary electrophoresis are frequently used to detect these analytes in complex mixtures. − UV absorbance detection is the most widely used detection method; however, its lack of selectivity necessitates great care in sample preparation to avoid interferences. An alternative is to derivatize adenine-containing compounds with chloroacetaldehyde and detect by laser-induced fluorescence, but the requirement of a long reaction time required and a UV laser limit the utility of this method.…”