Study of purified bacteria and viruses by pyrolysis mass spectrometry

Luo, Steven; Mohr, Jeff; Sickenberger, David; Hryncewich, Alex

doi:10.1002/(sici)1520-6521(1999)3:6<357::aid-fact5>3.0.co;2-j

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…[4,22,26]). Pyrolysis is also used, with chemical derivatization, in the US Army Chem-Bio Mass Spectrometer (CBMS) [27,28]. These studies were made in support of these end uses and studies were made finally using three strains of bacteria, E. coli, M. luteus and B. megaterium with the Py-GC/FID instrument.…”

Section: Py-gc/fid Of Three Bacteria Samplesmentioning

confidence: 99%

Quantitative assessment and optimization of parameters for pyrolysis of bacteria with gas chromatographic analysis

Schmidt

Tadjimukhamedov

Douglas

et al. 2006

Journal of Analytical and Applied Pyrolysis

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Section: Py-gc/fid Of Three Bacteria Samplesmentioning

confidence: 99%

Quantitative assessment and optimization of parameters for pyrolysis of bacteria with gas chromatographic analysis

Schmidt

Tadjimukhamedov

Douglas

et al. 2006

Journal of Analytical and Applied Pyrolysis

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“…Because cholesterol is not generally found in bacterial culture media, it can be thought of as a biomarker for the presence of viral aerosols. In another study using the same pyrolysis-methylation reaction demonstrated at Sandia, methylated cholesterol and fatty acids were detected for purified Coxiella burnetii and yellow fever [8]. In the case of unpurified agents, the lipids from the host cells would also be detected by pyrolysis-methylation.…”

Section: Viralmentioning

confidence: 99%

“…For bacillus anthracis, the causal agent of anthrax, it has been shown using pyrolysis-methylation that the sporulated form can be detected using dimethylated dipicolinic acid [8,32]. The sporulated form can contain from 5-15% by weight dipicolinic acid (DPA or 2,6-pyridinedicarboxylic acid) which can be methylated to 2Me-DPA (Dimethyl 2,6-pyridinedicarboxylate), and will be abbreviated in this report as mDPA.…”

Section: Sporulatedmentioning

confidence: 99%

“…(1 2 ) (1 1 ) (1 0 ) (9 ) (8 ) FID signal Temperature ramping can improve the analysis time for biomarker compounds as well. The following chromatograms illustrate the effect of different ramp rates on the separation of mDPA and a mixture which includes saturated and unsaturated of FAMEs.…”

Section: T Im E (M In U Tesmentioning

confidence: 99%

“…Direct pyrolysis produces limited biomarker peaks, reducing the ability to differentiate bacteria [7]. These instruments use either infrared or resistive heating pyrolysis and use large amounts of power in the pyrolysis step, and the infrared technique is slow and not suitable for chromatographic sample introduction [8]. The availability of a rapid and low-power pyrolyzer could reduce the size and power required of existing instrumentation.…”

Section: Introductionmentioning

confidence: 99%

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Miniature Sensors for Biological Warfare Agents using Fatty Acid Profiles: LDRD 10775 Final Report

Mowry¹,

Morgan²,

Frye-Mason³

et al. 2003

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Rapid detection and identification of bacteria and other pathogens is important for many civilian and military applications. The taxonomic significance, or the ability to differentiate one microorganism from another, using fatty acid content and distribution is well known. For analysis fatty acids are usually converted to fatty acid methyl esters (FAMEs). Bench-top methods are commercially available and recent publications have demonstrated that FAMEs can be obtained from whole bacterial cells in an in situ single-step pyrolysis/methylation analysis.This report documents the progress made during a three year Laboratory Directed Research and Development (LDRD) program funded to investigate the use of microfabricated components (developed for other sensing applications) for the rapid identification of bioorganisms based upon pyrolysis and FAME analysis. Components investigated include a micropyrolyzer, a microGC, and a surface acoustic wave (SAW) array detector. Results demonstrate that the micropyrolyzer can pyrolyze whole cell bacteria samples using only milliwatts of power to produce FAMEs from bacterial samples. The microGC is shown to separate FAMEs of biological interest, and the SAW array is shown to detect volatile FAMEs. Results for each component and their capabilities and limitations are presented and discussed. This project has produced the first published work showing successful pyrolysis/methylation of fatty acids and related analytes using a microfabricated pyrolysis device. 4 AcknowledgmentsThe authors would like to acknowledge the team that provided this project with components, without their work this LDRD would not have existed:

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Design, development, and performance of a fieldable chemical and biological agent detector

Hart

Wise

Griest

et al. 2000

Field Analyt. Chem. Technol.

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Study of purified bacteria and viruses by pyrolysis mass spectrometry

Cited by 9 publications

References 10 publications

Quantitative assessment and optimization of parameters for pyrolysis of bacteria with gas chromatographic analysis

Quantitative assessment and optimization of parameters for pyrolysis of bacteria with gas chromatographic analysis

Miniature Sensors for Biological Warfare Agents using Fatty Acid Profiles: LDRD 10775 Final Report

Design, development, and performance of a fieldable chemical and biological agent detector

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