Study of semi-automated solid-phase extraction for the determination of acaricide residues in honey by liquid chromatography

Simultaneous Determination of Amitraz, Bromopropylate, Coumaphos, Cymiazole and 2,4-Dimethylaniline in Korean Honey Samples by High-Performance Liquid Chromatography

“…These results are similar to previous results. 2,9 From these results and retention times of individual analytes, the conditions necessary for multiple UV detection were established as follows: 250 nm (0-5.5 min), 264 nm (5.5-7.0 min), 313 nm (7.0-10.5 min), 233 nm (10.5-16.5 min), and 289 nm (16.6-30.0 min). These conditions were applied to the HPLC analysis of the 1000 μg/L (each component) standard and the chromatogram is shown in Figure 2.…”

“…1 Even though very small amounts of the acaricides are used, a certain amount of the active ingredient can remain in the hive, resulting in the contamination of honey. 2 Many countries have established Maximum Residue Limit (MRL) for these four acaricides. 3 Korea has also established MRL.…”

“…Various kinds of mobile phases and extraction solutions used for analysis of acaricides resulted in different results. 2,3,[5][6][7] In this study, an additional effective extraction and simultaneous analysis method for amitraz, bromopropylate, coumaphos, cymiazole, and 2,4-dimethylaniline in honey were developed. This method involves simple sampling procedures followed by liquid-liquid extraction without purification of the samples and quantification of acaricide residues by HPLC with multiple UV detection.…”

Pesticide Residues in Bee Products

“…In the case of honey acetone , dichloromethane (Jimenez et al, 1998), ethyl acetate (Tsigouri et al, 2001), hexane (Gomis et al, 1996), methanol , a mixture of hexane-ethyl acetate (Tsigouri et al, 2001) have been occasionally used. With regard to the types of substrates used occasionally, the reverse phase C 18 was the most appropriate and chosen by most researchers for the extraction of insecticides, acaricides, herbicides, fungicides and other pesticides Bernal et al, 2000;Korta et al, 2001). Also microcolumn with Florisil gave good results in trials for determination of pyrethroid, OCP and OPP residues (Jimenez et al, 1998a) and C 8 in the determination of tau fluvalinate (Tsigouri et al, 2001).…”

“…The pH adjustment proved particularly important for the good recovery of some active substances. For example, coumaphos is unstable in an alkaline environment, as opposed to amitraz, the recovery increases with increasing pH values up to 11 (Korta et al, 2001). A comparison of the effectiveness of SPE and SE in pesticide residue analysis was conducted in two publications by Bernal et al (1996Bernal et al ( & 2000.…”