Study of some hormones and Partial purification of prolidase from serum of women with polycystic ovary syndrome

Abstract

This study was done by partially purification of prolidase from serum of patients with polycystic ovary syndrome by Gel filtration technique, and using sephadex G100 gel as a stationary phase. The degree of purification (15.1) fold, enzyme yield (95.5%) and specific activity (0.00176 IU/I), were carried out .Kinetics studies for the partial purified enzyme technique showed optimal concentration of substrate which was 5 mmol/l Km = 0.66ng and Vmax =0.80 mM, while optimum Temperature was (35C°) and optimum pH was (8). The molecular weight of the partial purified enzyme has been determined by gel electrophoresis method , in presence of polyacrylamide gel and sodium dodecyl sulphate (SDS_PAGE) which showed that the approximates molecular weight was (54KD),we found high level of prolactin in the patient with polycystic ovary syndrome which was(24.03) when in the control was( 10.09),the value of TSH in the patient was( 17.08) which is high value and in the control was( 1.49), the value of T4 in the patient was (100.2) and in control was (118.4),the value of T3 in the patient was (0.3)and in control was (1.3).Testosterone in patient was (0.391) and in control was (0.206).

Study of some parameters and Partial purification of prolidase from serum women preeclampsia

“…This modified assay is agree with study that done by Mr. wilk and Mr. Kalms (2017) for the optimum temperature [14]. -The influence of pH upon the activity of prolidase was investigated using buffer solution containing Tris -HCl with a pH rang (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)in this case conditions were conducted in the same manner as described earlier except that the p H was wearied as indicated in fig( 6)the optimum pH for prolidase was found to be (9)in Tris -HCl buffer, The result of pH varied with other studies ,the optimal pH for erythrocyte prolidase is also at pH 9.0 [15]. For many enzymes, the rate of catalysis varies with the substrate concentration the rate of catalysis is defined as the number of moles product formed per second at a fixed concentration of enzyme almost lineally proportional to (s)is small at high (s)is nearly independent of (s).to determine the effect of substrate concentration on the enzyme activity as series of concentration on the were performed where the concentration of substrate was varied fig (7).…”

“…Fig. 5: Effect of Temperature on Prolidase activity -Effect of pH :-The influence of pH upon the activity of prolidase was investigated using buffer solution containing Tris -HCl with a pH rang(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)in this case conditions were conducted in the same manner as described earlier except that the p H was wearied as indicated in fig(6)the optimum pH for prolidase was found to be (9)in Tris -HCl buffer, The result of pH varied with other studies ,the optimal pH for erythrocyte prolidase is also at pH 9.0[15].…”